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Home / Equine Research Bank / J Virol / Detection of proviral DNA in horse cells infected with equin...
Journal of virology1978; 26(3); 577-583; doi: 10.1128/JVI.26.3.577-583.1978

Detection of proviral DNA in horse cells infected with equine infectious anemia virus.

Abstract: Equine infectious anemia virus (EIAV) recently has been shown to possess a high-molecular-weight RNA genome and a virion reverse transcriptase. We completed the demonstration that EIAV is a retrovirus by showing the presence of proviral DNA in equine cells infected in vitro, but not in normal horse DNA. These studies were performed by using a highly representative cDNA probe synthesized by the virion polymerase. It was found that this cDNA reassociated extensively, and with high thermal stability, with either viral RNA or DNA extracted from infected cells, but showed no detectable reassociation with DNA from uninfected horse cells. Similarly, sequences related to EIAV were neither found in the DNA of four other Equus species, nor in a variety of other mammals including sheep, cows, pigs- dogs, cats, and humans; nor did EIAV cDNA hybridize with a variety of other retrovirus RNAs. These experiments were performed under conditions of very low stringency to enable detection of distantly related sequences, with a sufficient ratio of DAN to cDNA to allow detection of less than one viral copy per haploid genome. We conclude that EIAV is not an endogenous virus of the horse or of the other species tested.
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Publication Date: 1978-06-01 PubMed ID: 209211PubMed Central: PMC525883DOI: 10.1128/JVI.26.3.577-583.1978Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research demonstrates that the Equine Infectious Anemia Virus (EIAV) possesses proviral DNA in infected horse cells, but not in uninfected equine DNA, confirming it as a type of retrovirus. The study used a cDNA probe related to EIAV in several animals and found no significant reassociation, suggesting that EIAV is not an endogenous virus in these species.

Identification of Proviral DNA in EIAV

  • The researchers conducted this study by infecting equine cells in vitro with EIAV. The goal was to confirm the retroviral properties of EIAV, which include the presence of high-molecular-weight RNA genome and a virion reverse transcriptase.
  • The results were affirmative as proviral DNA was found in infected equine cells. However, such proviral DNA was not found in healthy horse DNA, providing the first evidence of EIAV’s retroviral nature.

Use of cDNA Probe

  • The researchers utilized a cDNA probe that was highly representative and synthesized by the virion polymerase to complete the research.
  • This cDNA probe was found to have an extensive and thermally stable reassociation with either the viral RNA or DNA derived from infected cells. On the other hand, no detectable reassociation was found with DNA from uninfected horse cells.

Testing Similarity of EIAV with Other Retroviruses

  • The researchers then expanded the study to check the presence of EIAV-related sequences in the DNA of four other Equus species, and mammals including sheep, cows, pigs, dogs, cats, and humans. This was done using very low stringency conditions to detect even distantly related sequences. They ensured a sufficient ratio of DNA to cDNA for detecting less than one viral copy per haploid genome.
  • However, no EIAV related sequences were found in the tested species’ DNA. Furthermore, the EIAV cDNA probe did not hybridize with RNAs from a variety of retroviruses. This led the researchers to conclude that EIAV is not an endogenous virus of any of these species.

Cite This Article

APA
Rice NR, Simek S, Ryder OA, Coggins L. (1978). Detection of proviral DNA in horse cells infected with equine infectious anemia virus. J Virol, 26(3), 577-583. https://doi.org/10.1128/JVI.26.3.577-583.1978

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 26
Issue: 3
Pages: 577-583

Researcher Affiliations

Rice, N R
    Simek, S
      Ryder, O A
        Coggins, L

          MeSH Terms

          • Animals
          • Cell Line
          • DNA / analysis
          • DNA, Viral / analysis
          • Horses
          • Infectious Anemia Virus, Equine / analysis
          • Infectious Anemia Virus, Equine / growth & development
          • Nucleic Acid Conformation
          • Nucleic Acid Hybridization
          • RNA, Viral / analysis
          • Species Specificity

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