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Veterinary world2022; 15(4); 827-833; doi: 10.14202/vetworld.2022.827-833

Determination of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from horses with respiratory manifestation.

Abstract: The World Health Organization considers multidrug-resistant (MDR) a major global threat. Horses harbor commensal isolates of this bacterial species and potentially serve as reservoirs for human MDR bacteria. This study investigated antimicrobial resistance in horses caused by extended-spectrum β-lactamase (ESBL)-producing . Unassigned: One hundred fifty-nine nasal swab samples were collected from horses with respiratory distress not treated with cefotaxime and erythromycin. Biochemical and serological identification was performed on all samples. Polymerase chain reaction (PCR) was used to detect , mucoviscosity-associated gene gA), uridine diphosphate galacturonate 4-epimerase gene e), and iron uptake system gene u), , , and genes. Sequence analysis and phylogenetic relatedness of randomly selected isolates carrying the gene were performed. Unassigned: Ten isolates of spp. were obtained from 159 samples, with an incidence of 6.28% (10 of 159). Based on biochemical and serological identification, was detected in 4.4% (7 of 159) of the samples. Using PCR, all tested isolates (n=7) carried the gene. By contrast, no isolates carried A, , and genes. The gene was detected in all test isolates. Moreover, all isolates did not harbor the or gene. Sequence analysis and phylogenetic relatedness reported that the maximum likelihood unrooted tree generated indicated the clustering of the test isolate with the other Gram-negative isolate . Finally, the sequence distance of the gene of the test isolate (generated by Lasergene) showed an identity range of 98.4-100% with the gene of the different test isolates. Unassigned: The misuse of antimicrobials and insufficient veterinary services might help generate a population of ESBL-producing in equines and humans, representing a public health risk.
Copyright: © Arafa, et al.
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Publication Date: 2022-04-06 PubMed ID: 35698500PubMed Central: PMC9178564DOI: 10.14202/vetworld.2022.827-833Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the prevalence of a drug-resistant bacterial strain, extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae, in horses with respiratory distress. The findings highlight the public health risks associated with the misuse of antimicrobials, potentially leading to the spread of this antibiotic-resistant bacteria to humans.

Research Methodology

  • The researchers collected nasal swab samples from 159 horses suffering from respiratory distress. These horses had not been treated with drugs cefotaxime and erythromycin.
  • All samples were subjected to biochemical and serological analysis to identify the presence of Klebsiella pneumoniae.
  • Polymerase chain reaction (PCR)—a technique to amplify specific DNA segments—was used to detect presence of various genes associated with the bacteria, such as K1, rmpA (mucoviscosity-associated gene), ugd (uridine diphosphate galacturonate 4-epimerase gene), iucA (iron uptake system gene), and a variety of other genes.

Findings of the Study

  • Out of 159 samples, ten yielded positive results for Klebsiella species—an incidence rate of 6.28%. Among these, seven samples, i.e., 4.4% were identified as Klebsiella pneumoniae.
  • PCR analysis showed that every tested Klebsiella pneumoniae isolate (total 7) carried the blaCTX-M gene—a gene that gives bacteria resistance to antibiotics. However, none of the isolates carry the K1, rmpA, and ugd genes.
  • All tested Klebsiella pneumoniae isolates had the iucA gene. But, none of the isolates hosted the rmpA or magA gene.
  • Sequence analysis indicated that maximum likelihood unrooted tree showed the tested isolate clustered together with another Gram-negative isolate Klebsiella variicola. The comparison of the genetic sequence of blaCTX-M gene in different test isolates showed an identity range of 98.4-100%.

Implications of the Study

  • The study shows that horses can be a host for ESBL-producing Klebsiella pneumoniae—a drug-resistant bacterial strain.
  • The presence of this bacteria in horses, caused potentially due to the misuse of antimicrobial drugs and insufficient veterinary services, may pose a public health threat by facilitating the bacteria’s spread to humans.

Cite This Article

APA
Arafa AA, Hedia RH, Dorgham SM, Ibrahim ES, Bakry MA, Abdalhamed AM, Abuelnaga ASM. (2022). Determination of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from horses with respiratory manifestation. Vet World, 15(4), 827-833. https://doi.org/10.14202/vetworld.2022.827-833

Publication

Veterinary world
ISSN: 0972-8988
NlmUniqueID: 101504872
Country: India
Language: English
Volume: 15
Issue: 4
Pages: 827-833

Researcher Affiliations

Arafa, Amany A
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Hedia, Riham H
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Dorgham, Sohad M
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Ibrahim, Eman S
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Bakry, Magdy A
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Abdalhamed, Abeer M
  • Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Egypt.
Abuelnaga, Azza S M
  • Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.

Conflict of Interest Statement

The authors declare that they have no competing interests.

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