Determination of intraspecies variations of the V2 region of the 16S rRNA gene of Streptococcus equi subsp. zooepidemicus.
Abstract: The 16S rRNA gene of 39 S. equi subsp. zooepidemicus strains and two S. equi subsp. equi strains was amplified by polymerase chain reaction and subsequently digested with the restriction enzyme Hinc II. A restriction profile with two fragments with sizes of 1250 bp and 200 bp could be observed for both S. equi subsp. equi strains and for 30 of the 39 S. equi subsp. zooepidemicus strains indicating a sequence variation within the V2 region of the 16S rRNA gene of the remaining nine S. equi subsp. zooepidemicus isolates. A segment of the 16S rRNA gene including the hypervariable V2 region of 11 S. equi subsp. zooepidemicus and two S. equi subsp. equi could be amplified by PCR and sequenced. The sequence of the V2 region of eight S. equi subsp. zooepidemicus strains appeared to be identical or almost identical to the sequence of the two S. equi subsp. equi strains. The sequence of the remaining three S equi subsp. zooepidemicus strains differed significantly from the sequence of S. equi subsp. equi. These differences allowed a division of S. equi subsp. zooepidemicus strains into two 16S rRNA types and might possibly have consequences for the taxonomic position of these phenotypically indistinguishable strains of one subspecies. A molecular typing could additionally be performed by amplification of the gene encoding the 16S-23S rRNA spacer region. A single amplicon of the spacer gene of 1100 bp could be observed for one S. equi subsp. zooepidemicus, an amplicon of 950 bp for two S. equi subsp. equi strains and 10 S. equi subsp. zooepidemicus strains, a amplicon of 780 bp for 27 S. equi subsp. zooepidemicus strains and a single amplicon of 600 bp for one S. equi subsp. zooepidemicus strain. The variations of the V2 region of the 16S rRNA gene and the size variations of the 16S-23S rRNA spacer gene were not related to each other. Both variations could be used for molecular typing of this species, possibly useful in epidemiological aspects.
Copyright 2000 Harcourt Publishers LtdCopyright 2000 Harcourt Publishers Ltd.
Publication Date: 2000-02-24 PubMed ID: 10684756DOI: 10.1053/rvsc.1999.0332Google Scholar: Lookup
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- Journal Article
Summary
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The research paper is about the identification of within-species variations of the V2 region of the 16S rRNA gene in the bacterium Streptococcus equi subsp. zooepidemicus, which may potentially change the taxonomic placement of these strains and be useful for molecular typing in epidemiology.
Study Methodology
- Samples were collected from 39 strains of S. equi subsp. zooepidemicus and 2 strains of S. equi subsp. equi.
- The 16S rRNA gene from these samples was amplified using Polymerase Chain Reaction (PCR) and then digested with the restriction enzyme Hinc II.
- Of these, 16S rRNA genes (comprising the V2 region) from 11 S. equi subsp. zooepidemicus and two S. equi subsp. equi were specifically amplified and sequenced.
Results and Findings
- A restriction profile with two fragments of sizes 1250 base pairs (bp) and 200 bp was observed in both S. equi subsp. equi strains, and 30 of the 39 S. equi subsp. zooepidemicus strains, suggesting sequence variation in the V2 region of the 16S rRNA gene in the remaining nine isolates of the latter.
- Eight of the sequenced S. equi subsp. zooepidemicus strains displayed nearly identical sequences compared to the S. equi subsp. equi strains. The other three expressed significant sequence differences.
- These differences allowed the researchers to divide S. equi subsp. zooepidemicus strains into two 16S rRNA types.
- The gene encoding the 16S-23S rRNA spacer region was also amplified as a method of molecular typing, producing different amplicon sizes among the samples.
- It was observed that the variations in the V2 region of the 16S rRNA gene and the size variations of the 16S-23S rRNA spacer gene are not related.
Implications and Conclusions
- The findings could potentially influence the taxonomic classification of indistinguishable S. equi subsp. zooepidemicus strains.
- The identified genetic variations can be used for targeted molecular typing of these strains, which can be beneficial for epidemiological studies and infection tracking.
Cite This Article
APA
Abdulmawjood A, Lämmler CH.
(2000).
Determination of intraspecies variations of the V2 region of the 16S rRNA gene of Streptococcus equi subsp. zooepidemicus.
Res Vet Sci, 68(1), 33-39.
https://doi.org/10.1053/rvsc.1999.0332 Publication
Researcher Affiliations
- Institut für Tierärztliche Nahrungsmittelkunde, Bakteriologie and Hygiene der Milch der Justus-Liebig-Universität Giebetaen, Giebetaen, Germany.
MeSH Terms
- Animals
- Base Sequence
- DNA, Ribosomal / genetics
- Deoxyribonucleases, Type II Site-Specific
- Genes, Bacterial
- Genetic Variation
- Horses
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction
- RNA, Ribosomal, 16S / genetics
- RNA, Ribosomal, 23S / genetics
- Species Specificity
- Streptococcus equi / classification
- Streptococcus equi / genetics
Citations
This article has been cited 5 times.- Arafa AA, Hedia RH, Ata NS, Ibrahim ES. Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt.. Vet World 2021 Jul;14(7):1808-1814.
- Mauchline TH, Knox R, Mohan S, Powers SJ, Kerry BR, Davies KG, Hirsch PR. Identification of new single nucleotide polymorphism-based markers for inter- and intraspecies discrimination of obligate bacterial parasites (Pasteuria spp.) of invertebrates.. Appl Environ Microbiol 2011 Sep;77(18):6388-94.
- Blum S, Elad D, Zukin N, Lysnyansky I, Weisblith L, Perl S, Netanel O, David D. Outbreak of Streptococcus equi subsp. zooepidemicus infections in cats.. Vet Microbiol 2010 Jul 29;144(1-2):236-9.
- Vossen A, Abdulmawjood A, Lämmler C, Weiss R, Siebert U. Identification and molecular characterization of beta-hemolytic streptococci isolated from harbor seals (Phoca vitulina) and grey seals (Halichoerus grypus) of the German North and Baltic Seas.. J Clin Microbiol 2004 Jan;42(1):469-73.
- Hassan AA, Khan IU, Abdulmawjood A, Lämmler C. Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis.. J Clin Microbiol 2001 Apr;39(4):1618-21.
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