Omneity® Pellets
All-In-One Vitamin & Mineral Pellet
Determination of phenylbutazone and oxyphenbutazone in plasma and urine samples of horses by high-performance liquid chromatography and gas chromatography-mass spectrometry.
Abstract: A method is described for the qualitative and quantitative determination of phenylbutazone and oxyphenbutazone in horse urine and plasma samples viewing antidoping control. A horse was administered intravenously with 3 g of phenylbutazone. For the qualitative determination, a screening by HPLC was performed after acidic extraction of the urine samples and the confirmation process was realized by GC-MS. Using the proposed method it was possible to detect phenylbutazone and oxyphenbutazone in urine for up to 48 and 120 h, respectively. For the quantitation of these drugs the plasma was deproteinized with acetonitrile and 20 microliters were injected directly into the HPLC system equipped with a UV detector and LiChrospher RP-18 column. The mobile phase used was 0.01 M acetic acid in methanol (45:55, v/v). The limit of detection was 0.5 microgram/ml for phenylbutazone and oxyphenbutazone and the limit of quantitation was 1.0 microgram/ml for both drugs. Using the proposed method it was possible to quantify phenylbutazone up to 30 h and oxyphenbutazone up to 39 h after administration.
Publication Date: 1996-04-12 PubMed ID: 8738024DOI: 10.1016/0378-4347(95)00508-0Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research describes a method for detecting and quantifying the presence of two drugs, phenylbutazone and oxyphenbutazone, in horse urine and plasma samples. This method was developed in the context of anti-doping controls in equine sports.
Study Overview
The study concentrated on developing a systematic technique to quantify phenylbutazone and oxyphenbutazone. These are non-steroidal anti-inflammatory drugs (NSAIDs) that are commonly used in veterinary practices, specifically for equine treatments.
- The purpose of the study was to contribute to and solidify the effectiveness of antidoping control in equine sports through improved methods of drug detection.
- The researchers administered a horse intravenously with 3g of phenylbutazone as part of the study.
Methodology
The methodology of the study was comprised of two main parts: the qualitative determination, or screening, and the quantitative determination, or measurement, of the drugs in question.
- For the qualitative determination, they performed High-Performance Liquid Chromatography (HPLC) after acid extraction of the urine samples. This technique was used to confirm the presence of the drugs.
- The confirmation process that followed the screening was executed by Gas Chromatography-Mass Spectrometry (GC-MS). This is another highly accurate scientific method often used in drug detection.
Results
The results obtained by the researchers demonstrated the efficiency and sensitivity of the proposed method.
- It was possible to detect phenylbutazone and oxyphenbutazone in urine up to 48 and 120 hours after administration, respectively.
- For the quantitation of these drugs, the plasma was deproteinized with acetonitrile, and 20 microliters were injected directly into the HPLC system.
- The limit of detection defined in this study was 0.5 micrograms/ml for both drugs, and the limit of quantitation was established at 1.0 micrograms/ml for both drugs.
- By using the proposed method, it was possible to quantify phenylbutazone up to 30 hours and oxyphenbutazone up to 39 hours after administration.
To conclude, the method described in this study provides a solid groundwork for doping control efforts in horse racing and other equine sports to ensure a fair and safe environment for all competitors.
Cite This Article
APA
Neto LM, Andraus MH, Salvadori MC.
(1996).
Determination of phenylbutazone and oxyphenbutazone in plasma and urine samples of horses by high-performance liquid chromatography and gas chromatography-mass spectrometry.
J Chromatogr B Biomed Appl, 678(2), 211-218.
https://doi.org/10.1016/0378-4347(95)00508-0 Publication
Researcher Affiliations
- Antidoping Laboratory, Jockey Club de São Paulo, Brazil.
MeSH Terms
- Acetic Acid
- Animals
- Chromatography, High Pressure Liquid / methods
- Chromatography, High Pressure Liquid / statistics & numerical data
- Chromatography, Thin Layer
- Female
- Gas Chromatography-Mass Spectrometry / methods
- Gas Chromatography-Mass Spectrometry / statistics & numerical data
- Horses / metabolism
- Methanol
- Oxyphenbutazone / analysis
- Oxyphenbutazone / blood
- Oxyphenbutazone / urine
- Phenylbutazone / analysis
- Phenylbutazone / blood
- Phenylbutazone / urine
Citations
This article has been cited 1 times.- Shibany KA, Tötemeyer S, Pratt SL, Paine SW. Equine hepatocytes: isolation, cryopreservation, and applications to in vitro drug metabolism studies. Pharmacol Res Perspect 2016 Oct;4(5):e00268.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
