Determination of serum anti-Müllerian hormone concentrations for the diagnosis of granulosa-cell tumours in mares.
Abstract: Endocrinological assays are important for evaluation of mares with granulosa-cell tumours (GCTs), and our research in mares indicates that anti-Müllerian hormone (AMH) may be a good biomarker for this type of ovarian tumour. Objective: To evaluate the use of serum AMH concentrations for endocrine diagnosis of GCTs in mares. Methods: Archived serum samples (n = 403) previously assayed for determination of serum inhibin, testosterone and progesterone concentrations (GCT panel) were assayed for serum AMH concentrations using a heterologous enzyme-linked immunosorbent assay previously validated by our laboratory. For a subset (n = 44) of these samples, a clinical diagnosis of GCT was confirmed by histopathology. Results: Overall, the sensitivity of AMH (98%) for detection of histologically confirmed GCTs was significantly (P<0.05) greater than that of either inhibin (80%) or testosterone (48%) or the combination of inhibin and testosterone (84%). Conclusions: Determination of serum AMH concentrations is a useful biomarker for detection of GCTs in the mare. Conclusions: Measurement of serum AMH concentrations can be used for diagnosis of GCTs in the mare. As serum AMH concentrations do not vary significantly during the oestrous cycle or pregnancy, interpretation of these results is not confounded by these physiological states.
© 2012 EVJ Ltd.
Publication Date: 2012-07-11 PubMed ID: 22779762DOI: 10.1111/j.2042-3306.2012.00594.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article presents a study on the use of anti-Müllerian hormone (AMH) as a biomarker for detecting granulosa-cell tumours (GCTs) in mares, indicating that AMH measurements provide a more sensitive and consistent means of diagnosis compared to prior methods.
Research Objective
- The primary objective of this research was to evaluate the effectiveness of serum AMH concentrations as an endocrine diagnostic method for GCTs in mares. This test aims to provide a more reliable, precise method for diagnosing this type of ovarian tumour in horses.
Research Method
- The researchers used a sample of 403 archived serum, which had been previously tested for inhibin, testosterone, and progesterone concentrations, which are substances commonly used to identify GCTs.
- The samples were retested, this time for AMH concentrations, using a heterologous enzyme-linked immunosorbent assay (a test that measures the concentration of a specific substance in a liquid sample), which had been previously authenticated by the researchers’ laboratory.
- A subset of these samples (44 in total) had been previously confirmed to have GCTs through histopathology (the examination of tissue to study the manifestations of disease).
Research Results
- The research evidenced that the use of AMH had a significantly greater sensitivity (98%) for the detection of GCTs compared to inhibin (80%), testosterone (48%), or the combination of both inhibin and testosterone (84%).
- This means that the measurement of serum AMH concentrations was more accurate and produced fewer false negatives than other tested substances.
Research Conclusions
- The study concluded that using serum AMH concentrations as a biomarker provides an efficacious method for the detection of GCTs in mares.
- A crucial advantage of this method is that AMH concentrations don’t significantly vary during different physiological states such as the oestrous cycle or pregnancy, hence, the interpretation of the results is not affected during these periods. This makes AMH a more reliable biomarker for the detection of GCTs.
Cite This Article
APA
Ball BA, Almeida J, Conley AJ.
(2012).
Determination of serum anti-Müllerian hormone concentrations for the diagnosis of granulosa-cell tumours in mares.
Equine Vet J, 45(2), 199-203.
https://doi.org/10.1111/j.2042-3306.2012.00594.x Publication
Researcher Affiliations
- Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, USA. b.a.ball@uky.edu
MeSH Terms
- Animals
- Anti-Mullerian Hormone / blood
- Estrous Cycle / blood
- Estrous Cycle / physiology
- Female
- Granulosa Cell Tumor / diagnosis
- Granulosa Cell Tumor / veterinary
- Horse Diseases / blood
- Horse Diseases / diagnosis
- Horses
- Inhibins / blood
- Pregnancy
- Retrospective Studies
- Sensitivity and Specificity
- Testosterone / blood
Citations
This article has been cited 8 times.- Straticò P, Hattab J, Guerri G, Carluccio A, Bandera L, Celani G, Marruchella G, Varasano V, Petrizzi L. Behavioral Disorders in Mares with Ovarian Disorders, Outcome after Laparoscopic Ovariectomy: A Case Series.. Vet Sci 2023 Jul 25;10(8).
- Dolin A, Schweiger P, Waselau M, Egerbacher M, Walter I. Immunohistochemical markers for equine granulosa cell tumors: a pilot study.. J Equine Sci 2023 Jun;34(2):37-46.
- Drozdzewska K, Gehlen H. Markers for internal neoplasia in the horse.. Vet Med Sci 2023 Jan;9(1):132-143.
- Colombe P, Béguin J, Benchekroun G, Le Roux D. Blood biomarkers for canine cancer, from human to veterinary oncology.. Vet Comp Oncol 2022 Dec;20(4):767-777.
- Goto A, Tagami M, Kato F, Suzuki T, Yamaga T, Murase H, Sato F, Tsogtgerel M, Niikura T, Moriyama T, Chiba A, Watanabe KI, Tsuzuki N, Nambo Y. Equine nonneoplastic abnormal ovary in a draft mare with high serum anti-Müllerian hormone: a case study.. J Equine Sci 2021 Dec;32(4):147-151.
- Tsogtgerel M, Tagami M, Watanabe K, Murase H, Hirosawa Y, Kobayashi Y, Nambo Y. Case report: The case of a 17 kg ovarian granulosa cell tumor in a Breton draft mare.. J Equine Sci 2021 Jun;32(2):67-72.
- Devick IF, Leise BS, McCue PM, Rao S, Hendrickson DA. Ovarian histopathology, pre- and post-operative endocrinological analysis and behavior alterations in 27 mares undergoing bilateral standing laparoscopic ovariectomy.. Can Vet J 2020 Feb;61(2):181-186.
- Umer S, Zhao SJ, Sammad A, Weldegebriall Sahlu B, Yunwei P, Zhu H. AMH: Could It Be Used as A Biomarker for Fertility and Superovulation in Domestic Animals?. Genes (Basel) 2019 Dec 4;10(12).
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