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Home / Equine Research Bank / Can J Vet Res / Development of a biologically immortalized equine stem cell ...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire2021; 85(4); 293-301;

Development of a biologically immortalized equine stem cell line.

Abstract: Bone repair in horses implies invasive surgeries and increased cost. Research on musculoskeletal disorders therapy in horses includes cell-based therapy with mesenchymal stromal cells (MSCs). Mesenchymal stromal cells can be obtained from bone marrow (BMMSCs). Unfortunately, BMMSCs have limited cell replication . The objective of this study was to develop a biologically immortalized equine stem cell line derived from bone marrow, with unlimited proliferation and the ability to differentiate into bone cells. Equine BMMSCs were transfected and immortalized with human telomerase reverse transcriptase (hTERT) gene. Cell passages from equine immortal BMMSCs were characterized by the presence of stemness CD markers and expression of multi-potent differentiation genes (OCT-4, SOX2, and NANOG). Equine immortal BMMSCs were incubated in osteogenic medium and bone cell differentiation was determined by alkaline phosphatase and von Kossa staining, and osteogenic gene expression (osteocalcin, Runx2, and osterix). Telomerase activity was determined by telomeric repeat amplification technique. Results showed that equine immortal BMMSCs were able to replicate up to passage 50 and maintain stem cell characteristics by the presence of CD90 and expression of multi-potent genes. Equine immortal BMMSCs were able to differentiate into bone cells, which was confirmed by the positive osteogenic staining and gene expression. Equine BMMSCs were successfully immortalized and maintained characteristics of stem cells and readily differentiated into osteogenic cells. Extending the life span of equine BMMSCs by transfection of the hTERT gene will revolutionize the clinical use of MSCs by making them available to orthopedic surgeons "off the shelf." La réparation osseuse chez les chevaux implique des chirurgies invasives et des coûts accrus. La recherche sur la thérapie des troubles musculosquelettiques chez les chevaux comprend la thérapie cellulaire avec des cellules stromales mésenchymateuses (CSM). Les CSM peuvent être obtenues à partir de la moelle osseuse (BMMSC). Malheureusement, les BMMSC ont une réplication cellulaire limitée . L’objectif de cette étude était de développer une lignée de cellules souches équines immortalisées biologiquement dérivées de la moelle osseuse, avec une prolifération illimitée et la capacité de se différencier en cellules osseuses. Les BMMSC équines ont été transfectées et immortalisées avec le gène de la transcriptase inverse de la télomérase humaine (hTERT). Les passages cellulaires des BMMSC immortels équins ont été caractérisés par la présence de marqueurs CD de souche et l’expression de gènes de différenciation multipotents (OCT-4, SOX2 et NANOG). Des BMMSC équins immortels ont été incubés dans un milieu ostéogénique et la différenciation des cellules osseuses a été déterminée par coloration à la phosphatase alcaline et de von Kossa, et l’expression des gènes ostéogéniques (ostéocalcine, Runx2 et osterix). L’activité de la télomérase a été déterminée par la technique d’amplification répétée des télomères. Les résultats ont montré que les BMMSC équins immortels étaient capables de se répliquer jusqu’au passage 50 et de maintenir les caractéristiques des cellules souches par la présence de CD90 et l’expression de gènes multipotents. Les BMMSC immortelles équines ont pu se différencier en cellules osseuses, ce qui a été confirmé par la coloration ostéogénique positive et l’expression des gènes. Les BMMSC équines ont été immortalisées avec succès et ont conservé les caractéristiques des cellules souches et facilement différenciées en cellules ostéogéniques. L’extension de la durée de vie des BMMSC équins par transfection du gène hTERT révolutionnera l’utilisation clinique des MSC en les mettant à la disposition des chirurgiens orthopédistes prête à l’emploi.(Traduit par Docteur Serge Messier).
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Publication Date: 2021-10-05 PubMed ID: 34602734PubMed Central: PMC8451704
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers successfully developed a biologically immortal stem cell line from horse bone marrow that has unlimited proliferation and can be made to develop into bone cells, which could revolutionize cell therapies for bone repair in horses.

Objective of the Study

  • The study aimed to develop a biologically immortal stem cell line derived from horse bone marrow. Stem cells have potential for use in the treatment of musculoskeletal disorders but they have limited replication abilities. By developing a stem cell line that can divide indefinitely, the researchers hope to provide a more effective treatment approach.

Methodology

  • The research team used equine bone marrow mesenchymal stromal cells (BMMSCs) which were transfected and immortalized using the human telomerase reverse transcriptase (hTERT) gene.
  • These immortalized BMMSCs were then characterized using stemness CD markers and the expression of multi-potent differentiation genes (OCT-4, SOX2, and NANOG).
  • The team conducted up to 50 cell passages from the immortal BMMSCs, with each passage tested for the ability to maintain stem cell characteristics and to differentiate into bone cells when incubated in osteogenic medium.

Findings

  • The results showed that the transformed cells could replicate up to 50 passages and still maintain their stem cell characteristics, as evidenced by the presence of the CD90 marker and the expression of multi-potent genes.
  • The immortalized BMMSCs were also able to differentiate into bone cells, which were confirmed by positive osteogenic staining and osteogenic gene expression (osteocalcin, Runx2, and osterix).

Conclusion and Implications

  • The team successfully developed a biologically immortalized equine stem cell line that maintained stem cell properties and was capable of differentiating into bone cells.
  • This breakthrough can revolutionize the clinical use of mesenchymal stromal cells, particularly for the application of bone repair, by making them readily available for orthopedic surgeons.

Cite This Article

APA
Nino-Fong R, Esparza Gonzalez BP, Rodriguez-Lecompte JC, Montelpare W, McD￮ L. (2021). Development of a biologically immortalized equine stem cell line. Can J Vet Res, 85(4), 293-301.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 1928-9022
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 85
Issue: 4
Pages: 293-301

Researcher Affiliations

Nino-Fong, Rodolfo
  • Department of Biomedical Sciences, College of Veterinary Medicine, Long Island University, 720 Northern Boulevard, Brookville, New York 11548, USA (Nino-Fong); Department of Health Management (Esparza Gonzalez, McD￮), Department of Pathology and Microbiology (Rodriguez-Lecompte), and Department of Applied Human Sciences (Montelpare), Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3.
Esparza Gonzalez, Blanca P
  • Department of Biomedical Sciences, College of Veterinary Medicine, Long Island University, 720 Northern Boulevard, Brookville, New York 11548, USA (Nino-Fong); Department of Health Management (Esparza Gonzalez, McD￮), Department of Pathology and Microbiology (Rodriguez-Lecompte), and Department of Applied Human Sciences (Montelpare), Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3.
Rodriguez-Lecompte, Juan Carlos
  • Department of Biomedical Sciences, College of Veterinary Medicine, Long Island University, 720 Northern Boulevard, Brookville, New York 11548, USA (Nino-Fong); Department of Health Management (Esparza Gonzalez, McD￮), Department of Pathology and Microbiology (Rodriguez-Lecompte), and Department of Applied Human Sciences (Montelpare), Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3.
Montelpare, William
  • Department of Biomedical Sciences, College of Veterinary Medicine, Long Island University, 720 Northern Boulevard, Brookville, New York 11548, USA (Nino-Fong); Department of Health Management (Esparza Gonzalez, McD￮), Department of Pathology and Microbiology (Rodriguez-Lecompte), and Department of Applied Human Sciences (Montelpare), Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3.
McD￮, Laurie
  • Department of Biomedical Sciences, College of Veterinary Medicine, Long Island University, 720 Northern Boulevard, Brookville, New York 11548, USA (Nino-Fong); Department of Health Management (Esparza Gonzalez, McD￮), Department of Pathology and Microbiology (Rodriguez-Lecompte), and Department of Applied Human Sciences (Montelpare), Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3.

MeSH Terms

  • Animals
  • Biomarkers / metabolism
  • Bone Marrow Cells
  • Cell Differentiation
  • Cell Line
  • Core Binding Factor Alpha 1 Subunit
  • Gene Expression Regulation / physiology
  • Horses
  • Mesenchymal Stem Cells / physiology
  • Osteocalcin
  • Osteogenesis

References

This article includes 40 references
  1. Caplan AI. New era of cell-based orthopedic therapies.. Tissue Eng Part B Rev 2009 Jun;15(2):195-200.
    pmc: PMC2817662pubmed: 19228082doi: 10.1089/ten.TEB.2008.0515google scholar: lookup
  2. Sen MK, Miclau T. Autologous iliac crest bone graft: should it still be the gold standard for treating nonunions?. Injury 2007 Mar;38 Suppl 1:S75-80.
    pubmed: 17383488doi: 10.1016/j.injury.2007.02.012google scholar: lookup
  3. Glynn ER, Londono AS, Zinn SA, Hoagland TA, Govoni KE. Culture conditions for equine bone marrow mesenchymal stem cells and expression of key transcription factors during their differentiation into osteoblasts.. J Anim Sci Biotechnol 2013 Oct 29;4(1):40.
    pmc: PMC3874597pubmed: 24169030doi: 10.1186/2049-1891-4-40google scholar: lookup
  4. Viswanathan S, Shi Y, Galipeau J, Krampera M, Leblanc K, Martin I, Nolta J, Phinney DG, Sensebe L. Mesenchymal stem versus stromal cells: International Society for Cell & Gene Therapy (ISCT®) Mesenchymal Stromal Cell committee position statement on nomenclature.. Cytotherapy 2019 Oct;21(10):1019-1024.
    pubmed: 31526643doi: 10.1016/j.jcyt.2019.08.002google scholar: lookup
  5. Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR. Allogeneic vs. autologous intra-articular mesenchymal stem cell injection within normal horses: Clinical and cytological comparisons suggest safety.. Equine Vet J 2020 Jan;52(1):144-151.
    pubmed: 31120574doi: 10.1111/evj.13136google scholar: lookup
  6. Gugjoo MB, Amarpal, Makhdoomi DM, Sharma GT. Equine Mesenchymal Stem Cells: Properties, Sources, Characterization, and Potential Therapeutic Applications.. J Equine Vet Sci 2019 Jan;72:16-27.
    pubmed: 30929778doi: 10.1016/j.jevs.2018.10.007google scholar: lookup
  7. Radtke CL, Nino-Fong R, Esparza Gonzalez BP, Stryhn H, McD￮ LA. Characterization and osteogenic potential of equine muscle tissue- and periosteal tissue-derived mesenchymal stem cells in comparison with bone marrow- and adipose tissue-derived mesenchymal stem cells.. Am J Vet Res 2013 May;74(5):790-800.
    pubmed: 23627394doi: 10.2460/ajvr.74.5.790google scholar: lookup
  8. Chanda D, Kumar S, Ponnazhagan S. Therapeutic potential of adult bone marrow-derived mesenchymal stem cells in diseases of the skeleton.. J Cell Biochem 2010 Oct 1;111(2):249-57.
    pmc: PMC2946500pubmed: 20506559doi: 10.1002/jcb.22701google scholar: lookup
  9. Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential.. Stem Cell Res Ther 2014 Feb 21;5(1):25.
    pmc: PMC4055040pubmed: 24559797doi: 10.1186/scrt414google scholar: lookup
  10. Branly T, Bertoni L, Contentin R, Rakic R, Gomez-Leduc T, Desancé M, Hervieu M, Legendre F, Jacquet S, Audigié F, Denoix JM, Demoor M, Galéra P. Characterization and use of Equine Bone Marrow Mesenchymal Stem Cells in Equine Cartilage Engineering. Study of their Hyaline Cartilage Forming Potential when Cultured under Hypoxia within a Biomaterial in the Presence of BMP-2 and TGF-ß1.. Stem Cell Rev Rep 2017 Oct;13(5):611-630.
    pubmed: 28597211doi: 10.1007/s12015-017-9748-ygoogle scholar: lookup
  11. Contentin R, Demoor M, Concari M, Desancé M, Audigié F, Branly T, Galéra P. Comparison of the Chondrogenic Potential of Mesenchymal Stem Cells Derived from Bone Marrow and Umbilical Cord Blood Intended for Cartilage Tissue Engineering.. Stem Cell Rev Rep 2020 Feb;16(1):126-143.
    pubmed: 31745710doi: 10.1007/s12015-019-09914-2google scholar: lookup
  12. Chu CR, Fortier LA, Williams A, Payne KA, McCarrel TM, Bowers ME, Jaramillo D. Minimally Manipulated Bone Marrow Concentrate Compared with Microfracture Treatment of Full-Thickness Chondral Defects: A One-Year Study in an Equine Model.. J Bone Joint Surg Am 2018 Jan 17;100(2):138-146.
    pmc: PMC6819026pubmed: 29342064doi: 10.2106/JBJS.17.00132google scholar: lookup
  13. Hotham WE, Henson FMD. The use of large animals to facilitate the process of MSC going from laboratory to patient-'bench to bedside'.. Cell Biol Toxicol 2020 Apr;36(2):103-114.
    pmc: PMC7196082pubmed: 32206986doi: 10.1007/s10565-020-09521-9google scholar: lookup
  14. De Angelis E, Cacchioli A, Ravanetti F, Bileti R, Cavalli V, Martelli P, Borghetti P. Gene expression markers in horse articular chondrocytes: Chondrogenic differentiaton IN VITRO depends on the proliferative potential and ageing. Implication for tissue engineering of cartilage.. Res Vet Sci 2020 Feb;128:107-117.
    pubmed: 31778851doi: 10.1016/j.rvsc.2019.10.024google scholar: lookup
  15. Carter-Arnold JL, Neilsen NL, Amelse LL, Odoi A, Dhar MS. In vitro analysis of equine, bone marrow-derived mesenchymal stem cells demonstrates differences within age- and gender-matched horses.. Equine Vet J 2014 Sep;46(5):589-95.
    pubmed: 23855680doi: 10.1111/evj.12142google scholar: lookup
  16. Stolzing A, Scutt A. Age-related impairment of mesenchymal progenitor cell function.. Aging Cell 2006 Jun;5(3):213-24.
    pubmed: 16842494doi: 10.1111/j.1474-9726.2006.00213.xgoogle scholar: lookup
  17. Ardanaz N, Vázquez FJ, Romero A, Remacha AR, Barrachina L, Sanz A, Ranera B, Vitoria A, Albareda J, Prades M, Zaragoza P, Martín-Burriel I, Rodellar C. Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints.. BMC Vet Res 2016 Mar 31;12:65.
    pmc: PMC4815220pubmed: 27029614doi: 10.1186/s12917-016-0692-xgoogle scholar: lookup
  18. Ursini TL, Amelse LL, Elkhenany HA, Odoi A, Carter-Arnold JL, Adair HS, Dhar MS. Retrospective analysis of local injection site adverse reactions associated with 230 allogenic administrations of bone marrow-derived mesenchymal stem cells in 164 horses.. Equine Vet J 2019 Mar;51(2):198-205.
    pubmed: 29992618doi: 10.1111/evj.12992google scholar: lookup
  19. Counter CM, Hahn WC, Wei W, Caddle SD, Beijersbergen RL, Lansdorp PM, Sedivy JM, Weinberg RA. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization.. Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8.
    pmc: PMC24516pubmed: 9843956doi: 10.1073/pnas.95.25.14723google scholar: lookup
  20. Zhang YW, Davis EG, Bai J. Determination of internal control for gene expression studies in equine tissues and cell culture using quantitative RT-PCR.. Vet Immunol Immunopathol 2009 Jul 15;130(1-2):114-9.
    pubmed: 19269038doi: 10.1016/j.vetimm.2009.01.012google scholar: lookup
  21. Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR.. Nucleic Acids Res 2002 May 1;30(9):e36.
    pmc: PMC113859pubmed: 11972351doi: 10.1093/nar/30.9.e36google scholar: lookup
  22. Radtke CL, Nino-Fong R, Rodriguez-Lecompte JC, Esparza Gonzalez BP, Stryhn H, McD￮ LA. Osteogenic potential of sorted equine mesenchymal stem cell subpopulations.. Can J Vet Res 2015 Apr;79(2):101-8.
    pmc: PMC4365701pubmed: 25852225
  23. Danisovic L, Oravcova L, Krajciova L, Varchulova Novakova Z, Bohac M, Varga I, Vojtassak J. Effect of long-term culture on the biological and morphological characteristics of human adipose tissue-derived stem Cells.. J Physiol Pharmacol 2017 Feb;68(1):149-158.
    pubmed: 28456779
  24. Li J, Liu W, Yao W. Immortalized Human Bone Marrow Derived Stromal Cells in Treatment of Transient Cerebral Ischemia in Rats.. J Alzheimers Dis 2019;69(3):871-880.
    pubmed: 31156178doi: 10.3233/JAD-190279google scholar: lookup
  25. Wongkajornsilp A, Sa-Ngiamsuntorn K, Hongeng S. Development of immortalized hepatocyte-like cells from hMSCs.. Methods Mol Biol 2012;826:73-87.
    pubmed: 22167641doi: 10.1007/978-1-61779-468-1_7google scholar: lookup
  26. Jiang T, Xu G, Wang Q, Yang L, Zheng L, Zhao J, Zhang X. In vitro expansion impaired the stemness of early passage mesenchymal stem cells for treatment of cartilage defects.. Cell Death Dis 2017 Jun 1;8(6):e2851.
    pmc: PMC5520885pubmed: 28569773doi: 10.1038/cddis.2017.215google scholar: lookup
  27. Xiaoxue Y, Zhongqiang C, Zhaoqing G, Gengting D, Qingjun M, Shenwu W. Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene.. Biochem Biophys Res Commun 2004 Mar 12;315(3):643-51.
    pubmed: 14975749doi: 10.1016/j.bbrc.2004.01.102google scholar: lookup
  28. Yalvaç ME, Yilmaz A, Mercan D, Aydin S, Dogan A, Arslan A, Demir Z, Salafutdinov II, Shafigullina AK, Sahin F, Rizvanov AA, Palotás A. Differentiation and neuro-protective properties of immortalized human tooth germ stem cells.. Neurochem Res 2011 Dec;36(12):2227-35.
    pubmed: 21785835doi: 10.1007/s11064-011-0546-7google scholar: lookup
  29. Piper SL, Wang M, Yamamoto A, Malek F, Luu A, Kuo AC, Kim HT. Inducible immortality in hTERT-human mesenchymal stem cells.. J Orthop Res 2012 Dec;30(12):1879-85.
    pubmed: 22674533doi: 10.1002/jor.22162google scholar: lookup
  30. Zhu X, Liu Z, Deng W, Zhang Z, Liu Y, Wei L, Zhang Y, Zhou L, Wang Y. Derivation and characterization of sheep bone marrow-derived mesenchymal stem cells induced with telomerase reverse transcriptase.. Saudi J Biol Sci 2017 Mar;24(3):519-525.
    pmc: PMC5372373pubmed: 28386176doi: 10.1016/j.sjbs.2017.01.022google scholar: lookup
  31. Zahedi M, Parham A, Dehghani H, Mehrjerdi HK. Stemness Signature of Equine Marrow-derived Mesenchymal Stem Cells.. Int J Stem Cells 2017 May 30;10(1):93-102.
    pmc: PMC5488781pubmed: 28222255doi: 10.15283/ijsc16036google scholar: lookup
  32. Machado C, Correa C, Medrado G, Leite M, Goes A. Ectopic expression of telomerase enhances osteopontin and osteocalcin expression during osteogenic differentiation of human mesenchymal stem cells from elder donors.. J Stem Cells Regen Med 2009;5(2):49-57.
    pmc: PMC3908137pubmed: 24693043doi: 10.46582/jsrm.0502010google scholar: lookup
  33. Gronthos S, Chen S, Wang CY, Robey PG, Shi S. Telomerase accelerates osteogenesis of bone marrow stromal stem cells by upregulation of CBFA1, osterix, and osteocalcin.. J Bone Miner Res 2003 Apr;18(4):716-22.
    pubmed: 12674332doi: 10.1359/jbmr.2003.18.4.716google scholar: lookup
  34. Dai F, Yang S, Zhang F, Shi D, Zhang Z, Wu J, Xu J. hTERT- and hCTLA4Ig-expressing human bone marrow-derived mesenchymal stem cells: in vitro and in vivo characterization and osteogenic differentiation.. J Tissue Eng Regen Med 2017 Feb;11(2):400-411.
    pubmed: 25047146doi: 10.1002/term.1924google scholar: lookup
  35. Skårn M, Noordhuis P, Wang MY, Veuger M, Kresse SH, Egeland EV, Micci F, Namløs HM, Håkelien AM, Olafsrud SM, Lorenz S, Haraldsen G, Kvalheim G, Meza-Zepeda LA, Myklebost O. Generation and characterization of an immortalized human mesenchymal stromal cell line.. Stem Cells Dev 2014 Oct 1;23(19):2377-89.
    pmc: PMC4172386pubmed: 24857590doi: 10.1089/scd.2013.0599google scholar: lookup
  36. Nakahara H, Misawa H, Hayashi T, Kondo E, Yuasa T, Kubota Y, Seita M, Kawamoto H, Hassan WA, Hassan RA, Javed SM, Tanaka M, Endo H, Noguchi H, Matsumoto S, Takata K, Tashiro Y, Nakaji S, Ozaki T, Kobayashi N. Bone repair by transplantation of hTERT-immortalized human mesenchymal stem cells in mice.. Transplantation 2009 Aug 15;88(3):346-53.
    pubmed: 19667936doi: 10.1097/TP.0b013e3181ae5ba2google scholar: lookup
  37. Sun L, Chiang JY, Choi JY, Xiong ZM, Mao X, Collins FS, Hodes RJ, Cao K. Transient induction of telomerase expression mediates senescence and reduces tumorigenesis in primary fibroblasts.. Proc Natl Acad Sci U S A 2019 Sep 17;116(38):18983-18993.
    pmc: PMC6754593pubmed: 31481614doi: 10.1073/pnas.1907199116google scholar: lookup
  38. Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S, Wright WE. Extension of life-span by introduction of telomerase into normal human cells.. Science 1998 Jan 16;279(5349):349-52.
    pubmed: 9454332doi: 10.1126/science.279.5349.349google scholar: lookup
  39. Liang XJ, Chen XJ, Yang DH, Huang SM, Sun GD, Chen YP. Differentiation of human umbilical cord mesenchymal stem cells into hepatocyte-like cells by hTERT gene transfection in vitro.. Cell Biol Int 2012 Feb;36(2):215-21.
    pubmed: 21988655doi: 10.1042/CBI20110350google scholar: lookup
  40. Robertson DM, Kalangara JP, Baucom RB, Petroll WM, Cavanagh HD. A reconstituted telomerase-immortalized human corneal epithelium in vivo: a pilot study.. Curr Eye Res 2011 Aug;36(8):706-12.
    pmc: PMC3149847pubmed: 21780919doi: 10.3109/02713683.2011.582662google scholar: lookup

Citations

This article has been cited 1 times.
  1. Chen Z, Meng D, Pang X, Guo J, Li T, Song J, Peng Y. Deer antler stem cells immortalization by modulation of hTERT and the small extracellular vesicles characters. Front Vet Sci 2024;11:1440855.
    doi: 10.3389/fvets.2024.1440855pubmed: 39430380google scholar: lookup
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