Development of an immunochromatographic assay for the β-adrenergic agonist feed additive zilpaterol.
Abstract: Zilpaterol is a β-adrenergic agonist feed additive approved in the United States to increase weight gain and improve feed efficiency of cattle. A zilpaterol immunochromatographic assay was developed as an economical and user-friendly rapid detection method for zilpaterol and validated using urine and tissue samples derived from animal studies. The assay sensitivity was 1.7-23.2 ng g or mL across a variety of feed and animal matrices and did not cross-react with clenbuterol or ractopamine. No sample pre-treatment of cattle and sheep urine was needed, but horse urine and feed required dilution; skeletal muscle required solvent extraction prior to testing. Of 32 incurred sheep urine samples tested, zilpaterol content was correctly identified in all but 2 samples. Horse urine containing >10 ng mL of incurred zilpaterol residue (n = 48) was correctly identified as zilpaterol positive. The assay correctly identified 0-day withdrawal sheep muscle samples as zilpaterol positive and the control and longer withdrawal day sheep muscle samples as negative. Zilpaterol was demonstrated to be stable in horse urine when stored at -20°C for 7 years.
Publication Date: 2018-06-06 PubMed ID: 29648941DOI: 10.1080/19440049.2018.1463568Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article is about the development and validation of a speedy, economical detection method for the cattle feed additive, zilpaterol. Known as an immunochromatographic assay, it has proven effective at detecting zilpaterol presence in samples from several animals and testing conditions.
Development and Validation of the Immunochromatographic Assay
- The researchers developed an immunochromatographic assay with the aim of creating an economical and easy-to-use rapid detection method for zilpaterol – a feed additive used to increase weight gain and improve feed efficiency in cattle.
- This assay was then validated using a variety of animal-derived urine and tissue samples.
Sensitivity and Cross-reactivity of the Assay
- It was found that the sensitivity of the assay varied between 1.7-23.2 ng/g or mL across a variety of feed and animal sample types.
- The assay was designed in such a way that it does not cross-react with other substances such as clenbuterol or ractopamine, which is a desirable feature as it ensures that the detection of zilpaterol is specific and not affected by the presence of these substances.
Sample Processing and Detection Accuracy
- In terms of sample processing, cattle and sheep urine samples required no prior treatment before being tested. However, horse urine and feed samples needed dilution, and skeletal muscle samples required a step of solvent extraction before testing.
- When the assay was put to the test, it was found to perform well in identifying zilpaterol content across a variety of samples. Out of 32 tested sheep urine samples, zilpaterol content was accurately identified in all but 2 samples. Similarly, horse urine samples with more than 10 ng/mL of incurred zilpaterol were accurately identified as being zilpaterol positive.
Evaluation of Zilpaterol Stability
- Additionally, the researchers tested the stability of zilpaterol in horse urine when stored at -20°C for a duration of 7 years. They found that zilpaterol remained stable, demonstrating the robustness of this feed additive under long-term storage conditions.
Cite This Article
APA
Shelver WL, Smith DJ.
(2018).
Development of an immunochromatographic assay for the β-adrenergic agonist feed additive zilpaterol.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 35(8), 1519-1529.
https://doi.org/10.1080/19440049.2018.1463568 Publication
Researcher Affiliations
- a USDA-ARS , Red River Valley Agricultural Research Center, Biosciences Research Laboratory , USA.
- a USDA-ARS , Red River Valley Agricultural Research Center, Biosciences Research Laboratory , USA.
MeSH Terms
- Adrenergic beta-Agonists / analysis
- Animal Feed / analysis
- Animals
- Cattle
- Chromatography, Affinity
- Dietary Supplements / analysis
- Food Additives / analysis
- Horses
- Sheep
- Trimethylsilyl Compounds / analysis
Citations
This article has been cited 1 times.- Luo H, Liu S, Shi L, Li Z, Bai Q, Du X, Wang L, Zha H, Li C. Paper-Based Fluidic Sensing Platforms for β-Adrenergic Agonist Residue Point-of-Care Testing. Biosensors (Basel) 2022 Jul 12;12(7).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
