Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses.
Abstract: An immunochromatographic test (BeICT) for the rapid detection of antibodies against Babesia equi was developed. It clearly differentiated B. equi-infected horses from B. caballi-infected and uninfected horses. The agreement with enzyme-linked immunosorbent assay results was 96.7% in the detection of field sera. The results suggest that BeICT is rapid, simple, reliable, and suitable for use to detect B. equi infection in the field.
Publication Date: 2004-01-13 PubMed ID: 14715778PubMed Central: PMC321651DOI: 10.1128/JCM.42.1.359-361.2004Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Antibodies
- Diagnosis
- Diagnostic Technique
- Disease
- Disease control
- Disease Diagnosis
- Disease Management
- Disease Outbreaks
- Disease Surveillance
- Disease Treatment
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Diseases
- Equine Health
- Horses
- Immunology
- Infection
- Infectious Disease
- Piroplasmosis
- Theileria equi
- Veterinary Care
- Veterinary Medicine
Summary
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This research paper discusses the creation of a quick immunochromatographic test, called BeICT, to detect antibodies against Babesia equi in horses.
Development of the Immunochromatographic Test
- The research revolves around the creation of a rapid detection test, known as an immunochromatographic test (BeICT), for the detection of antibodies associated with Babesia equi in horses.
- Babesia equi is a protozoan parasite that infects horses, causing equine piroplasmosis which is a serious disease.
- The test makes use of a recombinant equine merozoite antigen 2 (EMA-2), which is a specific protein identifiable by the immune system and is used to attract antibodies.
Effectiveness of the BeICT
- The researchers found that the BeICT successfully differentiated between horses infected with B. equi, B. caballi (another similar parasite) and completely uninfected horses.
- This is crucial in ensuring accurate diagnosis, especially as the symptoms of equine piroplasmosis can be similar to other illnesses.
Agreement with Other Testing Methods
- The BeICT results were compared with the results of an enzyme-linked immunosorbent assay (ELISA), a commonly used laboratory test to measure the amount of antibodies or antigens in a sample.
- The results showed a 96.7% agreement between the ELISA tests and the BeICT, revealing high levels of consistency and reliability.
Practical Application of the BeICT
- Given the fast and simple application of the BeICT, the research suggests that it is an apt method to use in field testing.
- It aids in rapid detection to promptly begin treatment and limit the spread to other horses.
Cite This Article
APA
Huang X, Xuan X, Xu L, Zhang S, Yokoyama N, Suzuki N, Igarashi I.
(2004).
Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses.
J Clin Microbiol, 42(1), 359-361.
https://doi.org/10.1128/JCM.42.1.359-361.2004 Publication
Researcher Affiliations
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
MeSH Terms
- Animals
- Antibodies, Protozoan / blood
- Babesiosis / diagnosis
- Babesiosis / veterinary
- Chromatography
- Enzyme-Linked Immunosorbent Assay
- Horse Diseases / diagnosis
- Horses
- Protozoan Proteins / immunology
- Recombinant Proteins / immunology
References
This article includes 8 references
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Citations
This article has been cited 12 times.- El-Sayed SAE, Rizk MA, Baghdadi HB, Ringo AE, Sambuu G, Nugraha AB, Igarashi I. Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA. PLoS One 2023;18(4):e0284535.
- Yang G, Zhou B, Chen K, Hu Z, Guo W, Wang X, Du C. Diagnostic Performance of Competitive ELISA and Western Blot Methods for the Detection of Antibodies against Theileria equi and Babesia caballi. Microorganisms 2022 Dec 21;11(1).
- Wang J, He K, Wu Z, Jin W, Wu W, Guo Y, Zhang W, Di W. Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies against Fasciola gigantica in buffalo. Front Vet Sci 2022;9:1004932.
- Yang G, Chen K, Guo W, Hu Z, Qi T, Liu D, Wang Y, Du C, Wang X. Development of a Test Card Based on Colloidal Gold Immunochromatographic Strips for Rapid Detection of Antibodies against Theileria equi and Babesia caballi. Microbiol Spectr 2022 Feb 23;10(1):e0241121.
- Aziz KJ, Al-Barwary LTO. Epidemiological Study of Equine Piroplasmosis (Theileria equi and Babesia caballi) by Microscopic Examination and Competitive-ELISA in Erbil Province North-Iraq. Iran J Parasitol 2019 Jul-Sep;14(3):404-412.
- Kerario II, Simuunza MC, Chenyambuga SW, Koski M, Hwang SG, Muleya W. Prevalence and risk factors associated with Theileria parva infection in cattle in three regions of Tanzania. Trop Anim Health Prod 2017 Dec;49(8):1613-1621.
- Lu Y, Guan G, Jiang T, Li Y, Yang J, Liu G, Luo J, Yin H, Liu Z. Development of an immunochromatographic strip for the serodiagnosis of Theileria infection in sheep. Parasit Vectors 2015 Dec 2;8:621.
- Mans BJ, Pienaar R, Latif AA. A review of Theileria diagnostics and epidemiology. Int J Parasitol Parasites Wildl 2015 Apr;4(1):104-18.
- Abdo J, Kristersson T, Seitzer U, Renneker S, Merza M, Ahmed J. Development and laboratory evaluation of a lateral flow device (LFD) for the serodiagnosis of Theileria annulata infection. Parasitol Res 2010 Oct;107(5):1241-8.
- Singh H, Mishra AK, Rao JR, Tewari AK. Comparison of indirect fluorescent antibody test (IFAT) and slide enzyme linked immunosorbent assay (SELISA) for diagnosis of Babesia bigemina infection in bovines. Trop Anim Health Prod 2009 Feb;41(2):153-9.
- Huang X, Xuan X, Verdida RA, Zhang S, Yokoyama N, Xu L, Igarashi I. Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses. Clin Vaccine Immunol 2006 May;13(5):553-5.
- Rogers MJ, McManus DP, Muhi S, Gordon CA. Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases. Clin Microbiol Rev 2021 Dec 15;34(4):e0032920.
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