Development of an indirect ELISA for the diagnosis of equine piroplasmosis.
Abstract: An indirect ELISA (iELISA) for the detection of specific anti-Theileria equi antibodies in horse serum was developed. Its performance showed good concordance (K= 0.79) when compared with a competitive ELISA recommended by the World Organisation for Animal Health. Horse serum samples from two provinces located in the north and east of Argentina (Formosa and Entre Rios, respectively) were analyzed by this iELISA. A high percentage of positive horses were found in Formosa, consistent with the climatic conditions of the region that are apt for the development of tick vectors. Surprisingly, seropositive animals were also detected, although in a lower proportion, in Entre Rios, which has a temperate weather and is presumably tick free. Breeding of thoroughbred racing horses is an important economic asset of Argentina. Since equine piroplasmosis is a constraint for horse export, the epidemiological situation in different regions of the country needs to be assessed for the implementation of control measures. The iELISA developed in this work provides a convenient tool for this type of study.
Publication Date: 2009-01-06 PubMed ID: 19120219DOI: 10.1196/annals.1428.029Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Antibodies
- Diagnosis
- Disease control
- Disease Diagnosis
- Disease Management
- Disease Outbreaks
- Disease Prevalence
- Disease Surveillance
- Disease Transmission
- Disease Treatment
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Diseases
- Equine Health
- Horse Breeds
- Infectious Disease
- Piroplasmosis
- Serodiagnosis
- Theileria equi
- Veterinary Medicine
- Veterinary Research
Summary
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This research paper chronicles the creation of an avoidable enzyme-linked immunosorbent assay (ELISA) used to detect specific antibodies related to Theileria equi in horse serum. The study found a high percentage of horses in certain regions of Argentina displayed positive results, indicating that equine Piroplasmosis is an issue that needs addressing to protect horse health and the industry economically.
Method of Research
- This study developed an indirect ELISA (iELISA) which is a method used for detecting antibodies related to certain diseases in serum.
- The objective was to find a convenient tool to detect specific anti-Theileria equi antibodies in horse serum, which is a pathogen associated with equine piroplasmosis.
- The researchers then compared the performance of this developed iELISA with the competitive ELISA recommended by the World Organisation for Animal Health. The result was satisfactory, with the developed iELISA showing good concordance (K= 0.79).
Application and Findings
- The developed iELISA was used for testing horse serum samples from two provinces in Argentina: Formosa and Entre Rios.
- The results showed a high percentage of positive horses in Formosa, matching with the climatic conditions of the region which are suitable for the development of tick vectors.
- Surprisingly, seropositive horses were also found in Entre Rios, a region with temperate climate and supposedly free from ticks.
Relevance of Research
- Breeding of thoroughbred racing horses is an important economic factor in Argentina. Equine piroplasmosis, a diseased caused by Theileria equi, is a barrier for horse exportation, and thus affects the economy.
- Given these findings, it is crucial to evaluate the epidemiological situation in various regions of the country in order to fit in necessary control measures for the disease.
- The iELISA test provides a convenient way to perform such assessments and contributes to the fight against equine piroplasmosis.
Cite This Article
APA
Asenzo G, Wilkowsky S, Barrandeguy M, Mesplet M, Benitez D, Florin-Christensen M.
(2009).
Development of an indirect ELISA for the diagnosis of equine piroplasmosis.
Ann N Y Acad Sci, 1149, 235-238.
https://doi.org/10.1196/annals.1428.029 Publication
Researcher Affiliations
- Institute of Virology, Center for Veterinary and Agronomic Research, National Institute of Agricultural Technology (INTA), Castelar, Buenos Aires, Argentina. gasenzo@cnia.inta.gov.ar
MeSH Terms
- Animals
- Antibodies, Protozoan / blood
- Babesiosis / diagnosis
- Babesiosis / veterinary
- Base Sequence
- DNA Primers
- Enzyme-Linked Immunosorbent Assay / methods
- Horse Diseases / diagnosis
- Horses
Citations
This article has been cited 3 times.- Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny. Pathogens 2020 Nov 8;9(11).
- Aziz KJ, Al-Barwary LTO. Epidemiological Study of Equine Piroplasmosis (Theileria equi and Babesia caballi) by Microscopic Examination and Competitive-ELISA in Erbil Province North-Iraq. Iran J Parasitol 2019 Jul-Sep;14(3):404-412.
- Mendoza FJ, Pérez-Écija A, Kappmeyer LS, Suarez CE, Bastos RG. New insights in the diagnosis and treatment of equine piroplasmosis: pitfalls, idiosyncrasies, and myths. Front Vet Sci 2024;11:1459989.
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