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Home / Equine Research Bank / J Anim Sci / Dietary conjugated linoleic acid supplementation alters skel...
Journal of animal science2021; 99(2); skab037; doi: 10.1093/jas/skab037

Dietary conjugated linoleic acid supplementation alters skeletal muscle mitochondria and antioxidant status in young horses.

Abstract: Conjugated linoleic acid (CLA) improves oxidative stress and mitochondrial biogenesis in various species but has not been thoroughly investigated in horses. We collected blood and muscle samples from lightly exercising horses before and 6 and 12 wk after receiving either soybean oil (CON; n = 5) or CLA (CLA; n = 5) supplementation. Samples were analyzed for markers of mitochondrial characteristics, antioxidant status, oxidative stress, and muscle damage. Data were analyzed using a linear model with repeated measures. In the triceps brachii (TB), citrate synthase (CS) activity was higher in CON than CLA horses (P = 0.003) but was unaffected by diet in the gluteus medius (GM). Integrative (relative to mg protein) cytochrome c oxidase (CCO) activity was higher in TB than the GM (P < 0.0001), while intrinsic (relative to CS) CCO was lower in the TB than the GM (P = 0.02) and tended to be lower in CON than CLA horses (P = 0.06). Neither CS nor integrative CCO activities were affected by time. In the GM, superoxide dismutase activity tended to increase in CON through week 12 (P = 0.10). Over both muscle groups, glutathione peroxidase activity tended to be higher in CON compared with CLA at week 12 (P = 0.06). Malondialdehyde was higher in the TB than the GM (P = 0.0004) but was unaffected by diet, while serum creatine kinase activity tended to be lower in CLA than CON horses (P = 0.07). These results suggest that CLA supplementation may lead to mitochondrial adaptations and prevent myofiber perturbation in skeletal muscle of young, lightly exercised horses.
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Publication Date: 2021-02-05 PubMed ID: 33539534PubMed Central: PMC7918170DOI: 10.1093/jas/skab037Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the impact of dietary conjugated linoleic acid (CLA) supplements on skeletal muscle mitochondria and antioxidant status in young horses, and suggests that CLA may lead to mitochondrial adaptations and protect against muscle fiber disturbances.

Research Methodology

  • Dietary supplementation of either soybean oil (labelled as CON) or conjugated linoleic acid (CLA) was administered to two separate groups of lightly exercising horses for a period of 6 and 12 weeks.
  • Blood and muscle samples were collected from the horses before and after the dietary intervention for analysis.
  • These samples were analyzed for markers of mitochondrial characteristics, antioxidant status, oxidative stress, and muscle damage.
  • Data was interpreted using a linear model with repeated measures for evaluation.

Key Findings

  • The activity of citrate synthase (CS), an enzyme critical for energy production in mitochondria, was found to be higher in the CON (soybean oil) group in comparison to the CLA group for the triceps brachii muscle (TB) but unaffected in the gluteus medius muscle (GM).
  • The activity of the protein essential for mitochondrial energy production, cytochrome c oxidase (CCO), was higher in the TB compared to the GM. Conversely, the ‘intrinsic’ CCO activity was lower in CON horses compared to CLA horses with a difference that bordered on significance.
  • There was no observed effect of time on either CS or integrating CCO activities.
  • Superoxide dismutase activity, an antioxidant enzyme, displayed a tendency to increase in CON horses in the GM throughout the 12 week period.
  • Another enzyme with antioxidant properties, glutathione peroxidase, tended to have higher activity in CON horses compared to CLA horses at the end of 12 weeks.
  • Malondialdehyde, a marker for oxidative stress, was higher in TB compared to GM, but not affected by the type of diet administered.
  • The activity of serum creatine kinase, a marker of muscle damage, tended to be lower in CLA horses as opposed to CON horses.

Conclusion

  • Findings suggest that CLA supplementation may bring about adaptations in mitochondria and also protect against disturbances in muscle fiber in young, lightly exercised horses.

Cite This Article

APA
Mrugala D, Leatherwood JL, Morris EF, Dickson EC, Latham CM, Owen RN, Beverly MM, Kelley SF, White-Springer SH. (2021). Dietary conjugated linoleic acid supplementation alters skeletal muscle mitochondria and antioxidant status in young horses. J Anim Sci, 99(2), skab037. https://doi.org/10.1093/jas/skab037

Publication

Journal of animal science
ISSN: 1525-3163
NlmUniqueID: 8003002
Country: United States
Language: English
Volume: 99
Issue: 2
PII: skab037

Researcher Affiliations

Mrugala, Daria
  • Texas A&M AgriLife Research and Department of Animal Science, Texas A&M University, College Station, TX, USA.
Leatherwood, Jessica L
  • Texas A&M AgriLife Research and Department of Animal Science, Texas A&M University, College Station, TX, USA.
Morris, Elizabeth F
  • School of Agricultural Sciences, Sam Houston State University, Huntsville, TX, USA.
Dickson, Emily C
  • Texas A&M AgriLife Research and Department of Animal Science, Texas A&M University, College Station, TX, USA.
Latham, Christine M
  • Texas A&M AgriLife Research and Department of Animal Science, Texas A&M University, College Station, TX, USA.
Owen, Randi N
  • Texas A&M AgriLife Research and Department of Animal Science, Texas A&M University, College Station, TX, USA.
Beverly, Marcy M
  • School of Agricultural Sciences, Sam Houston State University, Huntsville, TX, USA.
Kelley, Stanley F
  • School of Agricultural Sciences, Sam Houston State University, Huntsville, TX, USA.
White-Springer, Sarah H
  • Texas A&M AgriLife Research and Department of Animal Science, Texas A&M University, College Station, TX, USA.

MeSH Terms

  • Animals
  • Antioxidants
  • Dietary Supplements
  • Horses
  • Linoleic Acids, Conjugated / pharmacology
  • Mitochondria, Muscle
  • Muscle, Skeletal

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Citations

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