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Journal of animal science2007; 85(9); 2183-2189; doi: 10.2527/jas.2006-528

Dietary fish oil supplementation affects serum fatty acid concentrations in horses.

Abstract: Thirteen horses of Thoroughbred or Standardbred breeding were used to study the effect of dietary fish oil supplementation on blood lipid characteristics. Horses were assigned to either fish oil (n = 7) or corn oil (n = 6) treatment groups for 63 d. The fish oil contained 10.8% eicosapentaenoic acid (EPA) and 8% docosahexaenoic acid (DHA). Each horse received timothy hay and a mixed-grain concentrate at rates necessary to maintain BW. Oil (corn or fish) was top-dressed on the concentrate daily at a rate of 324 mg/ kg of BW. The n-6:n-3 ratio was approximately 3.6:1 for horses receiving the corn oil diet and 1.4:1 for horses receiving the fish oil diet. Horses were exercised 5 d/wk during the study. Before supplementation, there was no difference in the concentrations of any serum fatty acids between the 2 treatment groups. The mean basal concentrations of EPA and DHA on d 0 were 0.04 and 0.01 mg/mL, respectively. After 63 d, horses receiving the fish oil treatment, but not those receiving the corn oil treatment, had increased concentrations of EPA and DHA (P <0.05). Fish oil supplementation for 63 d also increased the concentrations of C22:0, C22:1, and C22:5 fatty acids (P <0.05). Overall, horses receiving fish oil had a decreased concentration of n-6 fatty acids (P <0.05) and a greater concentration of n-3 fatty acids (P <0.01), resulting in a lower n-6:n-3 fatty acid ratio after 63 d (P <0.05). Serum cholesterol concentrations increased (P <0.05) during the supplementation period in horses receiving the corn oil but not in horses receiving the fish oil. Compared with horses receiving corn oil, horses receiving fish oil had lower serum triglycerides at d 63 (P <0.05). These results demonstrate that 63 d of fish oil supplementation at 324 mg/kg of BW was sufficient to alter the fatty acid profile and blood lipid properties of horses receiving regular exercise.
Publication Date: 2007-05-15 PubMed ID: 17504965DOI: 10.2527/jas.2006-528Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This research article investigates the effects of supplementing horse diets with fish oil on their blood lipid characteristics, with the results indicating a significant influence, especially on fatty acid concentrations.

Study Design and Implementation

  • The study involved thirteen horses bred from either the Thoroughbred or Standardbred breed. The researchers divided the horses into two groups: seven received fish oil supplementation, and six were given corn oil.
  • The supplementation lasted 63 days and was dispensed on a daily basis at a rate of 324mg/kg of body weight. Each horse’s diet also included Timothy hay and a mixed-grain concentrate necessary for body weight maintenance. The oil (either corn or fish oil) was top-dressed on the concentrate.
  • The horses received regular exercise five days per week throughout the study period.

Profiles of Oils and Fatty Acid Ratios

  • The fish oil used in the supplementation contained 10.8% eicosapentaenoic acid (EPA) and 8% docosahexaenoic acid (DHA). These are omega-3 fatty acids known for their potential health benefits.
  • The n-6 to n-3 fatty acid ratio in the horse diets differed significantly between the two groups. The horses receiving corn oil had a ratio of approximately 3.6:1, while those on the fish oil diet had a lower ratio of about 1.4:1.

Study Findings

  • Before the supplementation began, both groups of horses had similar serum fatty acid concentrations. However, after 63 days of supplementation, a considerable difference was observed.
  • In horses receiving fish oil, there were increased concentrations of EPA, DHA, C22:0, C22:1, and C22:5 fatty acids. However, such an increase was not observed in horses that were given corn oil.
  • Horses on fish oil exhibited a lower concentration of n-6 fatty acids and a higher concentration of n-3 fatty acids, resulting in a lower n-6 to n-3 fatty acid ratio after 63 days of supplementation.
  • The serum cholesterol increased in horses on corn oil, but there was no such increase in those on fish oil. Additionally, by the end of the test period, the horses receiving fish oil showed lower serum triglycerides levels.

Conclusion

  • The study concludes that supplementing a horse’s diet with fish oil for a period of 63 days significantly alters the fatty acid profile and blood lipid properties, especially when these horses are subject to regular exercise. This outcome underscores the potential value of using fish oil as a supplement in horses’ diets for health and performance benefits.

Cite This Article

APA
O'Connor CI, Lawrence LM, Hayes SH. (2007). Dietary fish oil supplementation affects serum fatty acid concentrations in horses. J Anim Sci, 85(9), 2183-2189. https://doi.org/10.2527/jas.2006-528

Publication

ISSN: 1525-3163
NlmUniqueID: 8003002
Country: United States
Language: English
Volume: 85
Issue: 9
Pages: 2183-2189

Researcher Affiliations

O'Connor, C I
  • Department of Animal and Food Sciences, University of Kentucky, Lexington 40546, USA. oconn107@msu.edu
Lawrence, L M
    Hayes, S H

      MeSH Terms

      • Animal Feed
      • Animals
      • Cholesterol / blood
      • Corn Oil / administration & dosage
      • Docosahexaenoic Acids / administration & dosage
      • Docosahexaenoic Acids / blood
      • Docosahexaenoic Acids / metabolism
      • Eicosapentaenoic Acid / administration & dosage
      • Eicosapentaenoic Acid / blood
      • Eicosapentaenoic Acid / metabolism
      • Fatty Acids, Omega-3 / administration & dosage
      • Fatty Acids, Omega-3 / blood
      • Fatty Acids, Omega-3 / metabolism
      • Fatty Acids, Omega-6 / administration & dosage
      • Fatty Acids, Omega-6 / blood
      • Fatty Acids, Omega-6 / metabolism
      • Fish Oils / administration & dosage
      • Fish Oils / metabolism
      • Horses / blood
      • Horses / metabolism
      • Lipid Metabolism / drug effects
      • Male
      • Physical Conditioning, Animal / physiology
      • Random Allocation
      • Triglycerides / blood

      Citations

      This article has been cited 6 times.
      1. Hartwig S, Rankovic A, McCrae P, Gagliardi K, Burron S, Ellis J, Ma DWL, Shoveller AK. The effects of a plant-based and a plant- and marine-based n-3 oil supplement on behavioral reactivity, heart rate variability, and plasma fatty acid profile in young healthy horses. J Anim Sci 2025 Jan 4;103.
        doi: 10.1093/jas/skaf117pubmed: 40202435google scholar: lookup
      2. Hartwig S, Burron S, Richards T, Rankovic A, Ma DWL, Pearson W, Ellis J, Trevizan L, Seymour DJ, Shoveller AK. The effect of dietary camelina, flaxseed, and canola oil supplementation on skin fatty acid profile and immune and inflammatory responses in healthy adult horses. J Anim Sci 2025 Jan 4;103.
        doi: 10.1093/jas/skaf025pubmed: 39901745google scholar: lookup
      3. Robles M, Rousseau-Ralliard D, Dubois C, Josse T, Nouveau É, Dahirel M, Wimel L, Couturier-Tarrade A, Chavatte-Palmer P. Obesity during Pregnancy in the Horse: Effect on Term Placental Structure and Gene Expression, as Well as Colostrum and Milk Fatty Acid Concentration. Vet Sci 2023 Dec 4;10(12).
        doi: 10.3390/vetsci10120691pubmed: 38133242google scholar: lookup
      4. Fehlberg LK, Lattimer JM, Vahl CI, Drouillard JS, Douthit TL. Digestibility of diets containing calcium salts of fatty acids or soybean oil in horses. Transl Anim Sci 2020 Apr;4(2):txaa001.
        doi: 10.1093/tas/txaa001pubmed: 32705004google scholar: lookup
      5. Hallamaa R, Batchu K. Phospholipid analysis in sera of horses with allergic dermatitis and in matched healthy controls. Lipids Health Dis 2016 Mar 2;15:45.
        doi: 10.1186/s12944-016-0209-4pubmed: 26932514google scholar: lookup
      6. Ryan AS, Bailey-Hall E, Nelson EB, Salem N Jr. The hypolipidemic effect of an ethyl ester of algal-docosahexaenoic acid in rats fed a high-fructose diet. Lipids 2009 Sep;44(9):817-26.
        doi: 10.1007/s11745-009-3330-6pubmed: 19655188google scholar: lookup