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Home / Equine Research Bank / Biochem J / Differences between horse and human haemoglobins in effects ...
The Biochemical journal1990; 266(3); 897-900;

Differences between horse and human haemoglobins in effects of organic and inorganic anions on oxygen binding.

Abstract: Despite the fact that the horse is one of the more common domesticated animals, there are few reports dealing with the properties of its blood, and no comprehensive study has been performed on the reactivity of horse haemoglobin towards organic and inorganic ions. Here we report data on the effects of the organic phosphates D-glycerate-2,3-bisphosphate (2,3-DPG) and InsP6, and of chloride on the properties of horse haemoglobin. Thus the effect of saturating concentrations of 2,3-DPG on the oxygen affinity of horse haemoglobin is about 60% lower than with human adult haemoglobin under the same experimental conditions. The same applies also to InsP6, whose effect on oxygen binding to horse haemoglobin is decreased by about 55% compared with human adult haemoglobin. On the whole, horse haemoglobin appears to be much less sensitive to organic phosphates than previously believed. These results are discussed in the light of the primary structure of the molecule.
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Publication Date: 1990-03-15 PubMed ID: 2327974PubMed Central: PMC1131223
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the effects of certain organic and inorganic ions on the properties of horse haemoglobin, revealing distinct differences compared to human haemoglobin in response to these ions.

Objective of the Study

The main objective of this study was to conduct a comprehensive investigation into the effects of specific organic and inorganic ions on horse haemoglobin. The study sought to discover how these effects compare with those on human haemoglobin, as previous studies on this topic are limited.

Methods and Materials

  • The ions tested on the horse haemoglobin were organic phosphates D-glycerate-2,3-bisphosphate (2,3-DPG), InsP6, and an inorganic ion – chloride.
  • The researchers used saturating concentrations of these ions to study their effect on the oxygen affinity of horse haemoglobin.
  • These results were then compared against the effects observed under the same conditions on human adult haemoglobin.

Results and Findings

  • The researchers found that the effect of saturating concentrations of 2,3-DPG on the oxygen affinity of horse haemoglobin is approximately 60% lower than on human adult haemoglobin.
  • InsP6, another organic phosphate, also showed a reduced effect on oxygen binding to horse haemoglobin by about 55% compared with human haemoglobin.
  • Overall, the study concluded that horse haemoglobin exhibits a much lower sensitivity to organic phosphates than what was previously understood.

Implications

These findings are important because they lay the groundwork for a more in-depth understanding of the biological behavior of horse haemoglobin and its interaction with specific ions. This difference in sensitivity between horse and human haemoglobin to organic phosphates could have broader biological and clinical implications, especially in veterinary medicine and potentially in developing animal models for human diseases. The researchers plan on continuing this line of investigation in light of the primary structure of the molecule.

Cite This Article

APA
Giardina B, Brix O, Clementi ME, Scatena R, Nicoletti B, Cicchetti R, Argentin G, Condo SG. (1990). Differences between horse and human haemoglobins in effects of organic and inorganic anions on oxygen binding. Biochem J, 266(3), 897-900.

Publication

The Biochemical journal
ISSN: 0264-6021
NlmUniqueID: 2984726R
Country: England
Language: English
Volume: 266
Issue: 3
Pages: 897-900

Researcher Affiliations

Giardina, B
  • Department of Experimental Medicine and Biochemical Sciences, University of Rome, Italy.
Brix, O
    Clementi, M E
      Scatena, R
        Nicoletti, B
          Cicchetti, R
            Argentin, G
              Condo, S G

                MeSH Terms

                • 2,3-Diphosphoglycerate
                • Amino Acid Sequence
                • Animals
                • Chlorides / pharmacology
                • Diphosphoglyceric Acids / pharmacology
                • Hemoglobins / metabolism
                • Horses / blood
                • Humans
                • Molecular Sequence Data
                • Oxygen / metabolism

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                Citations

                This article has been cited 7 times.
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