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PloS one2014; 9(3); e92474; doi: 10.1371/journal.pone.0092474

Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.

Abstract: The role of intrinsic and extrinsic healing in injured tendons is still debated. In this study, we characterized cell plasticity, proliferative capacity, and migration characteristics as proxy measures of healing potential in cells derived from the peritenon (extrinsic healing) and compared these to cells from the tendon core (intrinsic healing). Both cell populations were extracted from horse superficial digital flexor tendon and characterized for tenogenic and matrix remodeling markers as well as for rates of migration and replication. Furthermore, colony-forming unit assays, multipotency assays, and real-time quantitative polymerase chain reaction analyses of markers of osteogenic and adipogenic differentiation after culture in induction media were performed. Finally, cellular capacity for differentiation towards a myofibroblastic phenotype was assessed. Our results demonstrate that both tendon- and peritenon-derived cell populations are capable of adipogenic and osteogenic differentiation, with higher expression of progenitor cell markers in peritenon cells. Cells from the peritenon also migrated faster, replicate more quickly, and show higher differentiation potential toward a myofibroblastic phenotype when compared to cells from the tendon core. Based on these data, we suggest that cells from the peritenon have substantial potential to influence tendon-healing outcome, warranting further scrutiny of their role.
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Publication Date: 2014-03-20 PubMed ID: 24651449PubMed Central: PMC3961373DOI: 10.1371/journal.pone.0092474Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study closely examines and compares the healing potentials of cell populations derived from the peritenon (the outer layer of a tendon) and the tendon core. The investigations suggest that cells from the peritenon display larger ability regarding adipogenic and osteogenic differentiation, faster migration, quicker replication, and a higher potential to differentiate into a myofibroblastic phenotype, possibly implying a substantial role in tendon healing.

Objective of the Study

  • The research attempts to contribute to the understanding of the healing process in injured tendons, specifically focusing on the differing roles and potentials of cells extracted from the peritenon and those from the tendon core.

Methodology

  • Cell characterization was done for two types of cells: those derived from the peritenon (contributing to extrinsic healing) and those derived from the tendon core (contributing to intrinsic healing). The cells were sourced from a horse’s superficial digital flexor tendon.
  • Cell plasticity, proliferative capacity, and migration characteristics stood as measures to determine their healing potential. Additionally, markers for tenogenic and matrix remodeling were studied.
  • Experiments including colony-forming unit assays, multipotency assays, and real-time quantitative polymerase chain reaction analyses were performed to investigate markers of osteogenic and adipogenic differentiation after culture in induction media.
  • The capacities of the cells to differentiate into a myofibroblastic phenotype were also evaluated.

Results and Conclusion

  • Results identified a capacity in both peritenon- and tendon-derived cell populations for adipogenic and osteogenic differentiation.
  • The peritenon-derived cells demonstrated a higher expression of progenitor cell markers, in comparison to cells from the tendon core.
  • Additionally, the peritenon cells migrated faster, replicated more quickly, and showed a higher potential to differentiate into a myofibroblastic phenotype.
  • Concluding their study, the researchers suggested that the peritenon-derived cells carry a significant potential to impact the outcome of tendon healing. Thus, the role and therapeutic application of these cells warrant further study and scrutiny.

Cite This Article

APA
Cadby JA, Buehler E, Godbout C, van Weeren PR, Snedeker JG. (2014). Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair. PLoS One, 9(3), e92474. https://doi.org/10.1371/journal.pone.0092474

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 9
Issue: 3
Pages: e92474
PII: e92474

Researcher Affiliations

Cadby, Jennifer A
  • Department of Orthopaedics, Balgrist Hospital, University of Zurich, Zurich, Switzerland; Department Health Sciences and Technology, Swiss Federal Institute of Technology, Zurich, Switzerland; Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Buehler, Evelyne
  • Department of Orthopaedics, Balgrist Hospital, University of Zurich, Zurich, Switzerland; Department Health Sciences and Technology, Swiss Federal Institute of Technology, Zurich, Switzerland.
Godbout, Charles
  • Osteoarthritis Research Unit, University of Montreal Hospital Research Centre (CRCHUM), Montreal, Q, Canada.
van Weeren, P René
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Snedeker, Jess G
  • Department of Orthopaedics, Balgrist Hospital, University of Zurich, Zurich, Switzerland; Department Health Sciences and Technology, Swiss Federal Institute of Technology, Zurich, Switzerland.

MeSH Terms

  • Adipogenesis
  • Animals
  • Biomarkers
  • Cell Culture Techniques
  • Cell Differentiation
  • Cell Movement
  • Cell Proliferation
  • Colony-Forming Units Assay
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Horses
  • Myofibroblasts / cytology
  • Myofibroblasts / metabolism
  • Osteogenesis
  • Stem Cells / cytology
  • Tendon Injuries / genetics
  • Tendon Injuries / metabolism
  • Tendon Injuries / therapy
  • Tendons / cytology
  • Tendons / metabolism
  • Wound Healing / physiology

Conflict of Interest Statement

The authors have declared that no competing interests exist.

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