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Home / Equine Research Bank / Microbiol Spectr / Differences in the Accessory Genomes and Methylomes of Strai...
Microbiology spectrum2022; 10(1); e0076421; doi: 10.1128/spectrum.00764-21

Differences in the Accessory Genomes and Methylomes of Strains of Streptococcus equi subsp. equi and of Streptococcus equi subsp. zooepidemicus Obtained from the Respiratory Tract of Horses from Texas.

Abstract: Streptococcus equi subsp. (SEE) is a host-restricted equine pathogen considered to have evolved from Streptococcus equi subsp. (SEZ). SEZ is promiscuous in host range and is commonly recovered from horses as a commensal. Comparison of a single strain each of SEE and SEZ using whole-genome sequencing, supplemented by PCR of selected genes in additional SEE and SEZ strains, was used to characterize the evolution of SEE. But the known genetic variability of SEZ warrants comparison of the whole genomes of multiple SEE and SEZ strains. To fill this knowledge gap, we utilized whole-genome sequencing to characterize the accessory genome elements (AGEs; i.e., elements present in some SEE strains but absent in SEZ or vice versa) and methylomes of 50 SEE and 50 SEZ isolates from Texas. Consistent with previous findings, AGEs consistently found in all SEE isolates were primarily from mobile genetic elements that might contribute to host restriction or pathogenesis of SEE. Fewer AGEs were identified in SEZ because of the greater genomic variability among these isolates. The global methylation patterns of SEE isolates were more consistent than those of the SEZ isolates. Among homologous genes of SEE and SEZ, differential methylation was identified only in genes of SEE encoding proteins with functions of quorum sensing, exopeptidase activity, and transitional metal ion binding. Our results indicate that effects of genetic mobile elements in SEE and differential methylation of genes shared by SEE and SEZ might contribute to the host specificity of SEE. Strangles, caused by the host-specific bacterium Streptococcus equi subsp. (SEE), is the most commonly diagnosed infectious disease of horses worldwide. Its ancestor, Streptococcus equi subsp. (SEZ), is frequently isolated from a wide array of hosts, including horses and humans. A comparison of the genomes of a single strain of SEE and SEZ has been reported, but sequencing of further isolates has revealed variability among SEZ strains. Thus, the importance of this study is that it characterizes genomic and methylomic differences of multiple SEE and SEZ isolates from a common geographic region (, Texas). Our results affirm many of the previously described differences between the genomes of SEE and SEZ, including the role of mobile genetic elements in contributing to host restriction. We also provide the first characterization of the global methylome of Streptococcus equi and evidence that differential methylation might contribute to the host restriction of SEE.
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Publication Date: 2022-01-12 PubMed ID: 35019696PubMed Central: PMC8754150DOI: 10.1128/spectrum.00764-21Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the genetic and epigenetic differences between two pathogenic subspecies of Streptococcus equi bacteria that are found in horses, with the aim to understand how specific strains have evolved to specialize in particular host species.

Research Objective

  • The goal of this research was to understand more clearly how the streptococcus equi subspecies equi (SEE) bacteria have evolved to limit their infection host to only horses, whereas the subspecies zooepidemicus (SEZ) can infect a broader range of hosts.
  • To achieve this, researchers compared the accessory genome elements (AGEs) and the methylation patterns – components of the total genome – of 50 SEE and SEZ bacterial isolates taken from horses in Texas. AGEs are parts of the genome which may be present in certain strains but not in others, and could offer insights into genetic adaptations.

Findings

  • They found that the AGEs consistently found in all SEE isolates came from mobile genetic elements which could contribute to host restriction or pathogenesis of SEE. However, the SEZ subspecies exhibited fewer AGEs, potentially due to increased genetic variability among these isolates.
  • The global methylation patterns of SEE isolates were more consistent than those of SEZ isolates. Also, in genes common to both subspecies, only SEE showed differential methylation specifically in genes encoding proteins with functions of quorum sensing, exoprotease activity, and transitional metal ion binding.

Conclusion

  • This study concluded that the effects of mobile genetic elements and differential methylation might be key factors contributing to the host specificity of the SEE bacteria, confirming some previous findings and presenting novel insights.
  • This research not only affirmed many previously described differences between the genomes of SEE and SEZ subspecies but also demonstrated for the first time the differential DNA methylation patterns of Streptococcus equi genomes, suggesting their potential role in determining host specificity of the bacteria.

Cite This Article

APA
Morris ERA, Wu J, Bordin AI, Lawhon SD, Cohen ND. (2022). Differences in the Accessory Genomes and Methylomes of Strains of Streptococcus equi subsp. equi and of Streptococcus equi subsp. zooepidemicus Obtained from the Respiratory Tract of Horses from Texas. Microbiol Spectr, 10(1), e0076421. https://doi.org/10.1128/spectrum.00764-21

Publication

Microbiology spectrum
ISSN: 2165-0497
NlmUniqueID: 101634614
Country: United States
Language: English
Volume: 10
Issue: 1
Pages: e0076421
PII: e00764-21

Researcher Affiliations

Morris, Ellen Ruth A
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Wu, Jing
  • Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Bordin, Angela I
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Lawhon, Sara D
  • Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Cohen, Noah D
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.

MeSH Terms

  • Animals
  • DNA Methylation
  • Epigenome
  • Genetic Variation
  • Genome, Bacterial
  • Horse Diseases / microbiology
  • Horses
  • Respiratory System / microbiology
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / veterinary
  • Streptococcus / classification
  • Streptococcus / genetics
  • Streptococcus / isolation & purification
  • Streptococcus equi / classification
  • Streptococcus equi / genetics
  • Streptococcus equi / isolation & purification
  • Texas

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 2 times.
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