Different distribution of Cryptosporidium species between horses and donkeys.
Abstract: Few studies have been conducted on the distribution of Cryptosporidium species and subtypes in equine animals. In this study, 878 stool specimens were collected during 2015-2019 from 551 donkeys and 327 horses in Shandong, Xinjiang, and Inner Mongolia, China and screened for Cryptosporidium spp. by PCR analysis of the small subunit rRNA gene. The Cryptosporidium species presented were identified by sequence analysis of the PCR products and subtyped by sequence analysis of the 60 kDa glycoprotein gene. The infection rates of Cryptosporidium spp. in horses and donkeys were 3.1% (10/327) and 14.5% (80/551), respectively. Four Cryptosporidium species/genotypes were identified, including C. parvum (in 5 horses), C. hominis (in 75 donkeys), Cryptosporidium horse genotype (in 5 horses and 4 donkeys) and a new genotype that is genetically related to Cryptosporidium mink genotype (in 1 donkey). All C. parvum isolates were subtyped as IIdA19G1, C. hominis as IkA16G1, and horse genotype as VIaA15G4. Data from this study indicate that four Cryptosporidium species are circulating in horses and donkeys in the study areas, with C. hominis as a dominant Cryptosporidium species in only donkeys. Attention should be paid to reduce the transmission of these zoonotic Cryptosporidium spp.
Copyright © 2019. Published by Elsevier B.V.
Publication Date: 2019-07-08 PubMed ID: 31295579DOI: 10.1016/j.meegid.2019.103954Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The researchers laid out an investigation to understand the distribution of Cryptosporidium species in equine animals, specifically horses and donkeys. Cryptosporidium are parasites that can cause intestinal problems. The study identified four types of Cryptosporidium in the animals and found a higher infection rate in donkeys than horses.
Methodology
- The researchers utilized over 800 stool samples from both donkeys and horses collected between 2015 to 2019 from different Chinese regions.
- Polymerase Chain Reaction (PCR) was performed on the collected specimens to detect Cryptosporidium species. This is a common method used to amplify and thus detect the presence of specific genes.
- The identified Cryptosporidium species underwent sequence analysis, a procedure that determines the precise order of nucleotides in a DNA molecule.
- Subtyping was completed using the sequence analysis of the 60 kDa glycoprotein gene, assisting in further determination of the Cryptosporidium species present.
Findings
- The study found the infection rates of Cryptosporidium in horses and donkeys to be 3.1% and 14.5% respectively.
- Four Cryptosporidium species/genotypes were identified. These were C. parvum (in 5 horses), C. hominis (in 75 donkeys), Cryptosporidium horse genotype (in 5 horses and 4 donkeys) and a new genotype genetically related to Cryptosporidium mink genotype (in 1 donkey).
- Subtyping identified all C. parvum isolates as IIdA19G1, C. hominis as IkA16G1, and horse genotype as VIaA15G4.
Implications
- The paper concludes that different Cryptosporidium species are present in horses and donkeys across the studied Chinese regions, with C. hominis being the dominant Cryptosporidium species in donkeys.
- The research emphasizes the need for measures to control and manage the transmission of these zoonotic Cryptosporidium species which can also infect humans and transmit diseases.
Cite This Article
APA
Li F, Su J, Chahan B, Guo Q, Wang T, Yu Z, Guo Y, Li N, Feng Y, Xiao L.
(2019).
Different distribution of Cryptosporidium species between horses and donkeys.
Infect Genet Evol, 75, 103954.
https://doi.org/10.1016/j.meegid.2019.103954 Publication
Researcher Affiliations
- Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China.
- State Key Laboratory of Bioreactor Engineering, School of Resource and Environmental, East China University of Science and Technology, Shanghai 200237, China.
- Department of Animal Parasitology, Xinjiang Agricultural University School of Veterinary Medicine, Uygur, Xinjiang 830052, China.
- Department of Animal Parasitology, Xinjiang Agricultural University School of Veterinary Medicine, Uygur, Xinjiang 830052, China.
- National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Done-E Country, Shandong 252201, China.
- Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China.
- Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China.
- Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China.
- Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China. Electronic address: yyfeng@scau.edu.cn.
- Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China. Electronic address: lxiao1961@gmail.com.
MeSH Terms
- Animals
- Cryptosporidium / classification
- Cryptosporidium / genetics
- Cryptosporidium / isolation & purification
- Equidae / parasitology
- Genes, Protozoan
- Horses / parasitology
Citations
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