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Home / Equine Research Bank / Vet Res Commun / Different gradients for neurotransmitter metabolites and pro...
Veterinary research communications1989; 13(6); 413-419; doi: 10.1007/BF00402561

Different gradients for neurotransmitter metabolites and protein in horse cerebrospinal fluid.

Abstract: The serotonin metabolite, 5-hydroxyindoleacetic acid (5-HIAA) and the dopamine metabolite, homovanillic acid (HVA) in the cerebrospinal fluid (CSF) of seven clinically normal horses were evaluated with reverse phase high pressure liquid chromatography and electrochemical detection. Comparisons of the neurotransmitter metabolite concentrations were made on CSF collected simultaneously from the atlanto-occipital and lumbosacral regions. There were significantly higher amounts of 5-HIAA and HVA in atlanto-occipital CSF than in lumbosacral CSF. Mean 5-HIAA concentrations in atlanto-occipital and lumbosacral CSF were 206.1 +/- 37.0 nmol/L and 68.5 +/- 12.6 nmol/L, respectively. The mean HVA concentration in atlanto-occipital CSF was 254.4 +/- 50.4 nmol/L. No HVA was detected in lumbosacral CSF. In contrast, significantly greater protein concentrations were present in lumbosacral CSF than in atlanto-occipital CSF. Lumbosacral and atlanto-occipital CSF contained 32.1 +/- 3.7 mg protein/dl and 25.0 +/- 1.3 mg protein/dl, respectively. Thus, between the atlanto-occipital and lumbosacral space of the horses, a rostrocaudal gradient existed for neurotransmitter metabolites and a caudorostral gradient existed for protein.
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Publication Date: 1989-01-01 PubMed ID: 2483597DOI: 10.1007/BF00402561Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines the concentration of serotonin and dopamine metabolites alongside protein levels in the cerebrospinal fluid (CSF) of horses. The researchers found higher levels of these metabolites in the CSF in the upper part of the spinal cord, compared to the lower part, which conversely, had higher protein concentrations.

Objective and Methodology:

  • The research aimed to understand the variation in concentration of two neurotransmitter metabolites, 5-hydroxyindoleacetic acid (5-HIAA) – a serotonin metabolite, and homovanillic acid (HVA) – a dopamine metabolite, in the cerebrospinal fluid of horses.
  • The researchers also wanted to examine how protein levels vary in different parts of the CSF. For this, they used seven healthy horses as their study sample.
  • The metabolite concentrations were evaluated using techniques known as reverse phase high pressure liquid chromatography and electrochemical detection.
  • The researchers analysed the CSF collected simultaneously from two regions of the spinal cord: atlanto-occipital (upper part) and lumbosacral (lower part).

Findings:

  • The findings showed higher amounts of 5-HIAA and HVA in the atlanto-occipital CSF compared to the lumbosacral CSF.
  • In terms of specific numbers, the mean concentration of 5-HIAA was 206.1 nmol/L in the atlanto-occipital CSF, while it was 68.5 nmol/L in lumbosacral CSF. The mean HVA concentration in the atlanto-occipital CSF was 254.4 nmol/L, but no HVA was detected in the lower part of the spinal cord.
  • On the other hand, significantly greater concentrations of protein were observed in the lumbosacral CSF than in the atlanto-occipital CSF. The mean protein level was 32.1 mg/dL in the lumbosacral CSF and 25.0 mg/dL in the atlanto-occipital CSF.

Conclusions:

  • The researchers concluded that there is a rostrocaudal gradient (from head to tail, or top to bottom) for neurotransmitter metabolites in the CSF, meaning the concentrations were higher in the upper part of the spinal cord.
  • Conversely, a caudorostral gradient (from tail to head, or bottom to top) existed for protein, as the concentrations were higher in the lower part of the spinal cord.

This study helps us understand how neurotransmitter metabolite and protein concentrations change along the spinal cord. This could prove vital in studying various neurological diseases in animals and potentially in humans.

Cite This Article

APA
Vaughn DM, Smyth GB. (1989). Different gradients for neurotransmitter metabolites and protein in horse cerebrospinal fluid. Vet Res Commun, 13(6), 413-419. https://doi.org/10.1007/BF00402561

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 13
Issue: 6
Pages: 413-419

Researcher Affiliations

Vaughn, D M
  • Medicinal Biochemistry Laboratory, College of Veterinary Medicine, Auburn University, Alabama 36849-5525.
Smyth, G B

    MeSH Terms

    • Animals
    • Cerebrospinal Fluid Proteins / metabolism
    • Chromatography, High Pressure Liquid
    • Female
    • Homovanillic Acid / cerebrospinal fluid
    • Horses / cerebrospinal fluid
    • Hydroxyindoleacetic Acid / cerebrospinal fluid
    • Lumbosacral Region
    • Male
    • Neurotransmitter Agents / cerebrospinal fluid
    • Spinal Canal

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    Citations

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