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Home / Equine Research Bank / Histochemistry / Differential distribution of immunoreactive S-100 protein in...
Histochemistry1994; 102(3); 241-245; doi: 10.1007/BF00268901

Differential distribution of immunoreactive S-100 protein in mammalian testis.

Abstract: The present study deals with the immunohistochemical localization of S-100 protein in the testes of seven mammalian species including rat, cat, dog, pig, sheep, cattle and horse. Significant differences are demonstrated in the cellular distribution and intensity of immunoreaction for the protein. In bull, ram, boar and cat testes S-100 protein was localized in the cytoplasm and nuclei of Sertoli cells. A particularly intense staining was seen in the modified Sertoli cells of the terminal tubular segment. With the exception of the cat and horse S-100 protein immunoreactivity was additionally found in epithelial cells of the straight testicular tubules and in the epithelial cells of the rete testis. Endothelial cells of capillaries, veins and lymphatic vessels are regularly S-100 immunoreactive in ruminants. Leydig cells were found to be strongly positive for S-100 protein in the cat and rat testes and to a lower degree in pig and horse testes. Finally a distinct immunostaining of peritubular cells was restricted to the testis of dogs and rats. The remarkable species-specific variations of immunoreactivity for S-100 protein in different cell types of the testis support the hypothesis that S-100 protein is multifunctional protein and may have a different function in testicular physiology.
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Publication Date: 1994-09-01 PubMed ID: 7868366DOI: 10.1007/BF00268901Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article reveals that there is significant variance in the cellular distribution and intensity of the S-100 protein in the testes of different mammalian species like rats, cats, dogs, pigs, sheep, cattle, and horses. This suggests that the S-100 protein has multiple functions which can vary depending on the physiology of the species.

Immunohistochemical Localization of S-100 Protein

  • The researchers used immunohistochemical techniques to locate the presence and intensity of S-100 protein in the testes of different mammalian species.
  • The species included in the study were: rat, cat, dog, pig, sheep, cattle, and horse.
  • Differences were noted in the cellular distribution of the S-100 protein among these species and the intensity of the immunoreactive response to the protein.

Variations in Protein Distribution

  • In the testes of bulls, rams, boars, and cats, the S-100 protein was localized mostly in the Sertoli cells, with particularly intense staining noted within the modified Sertoli cells of the terminal tubular segment.
  • Except for horses and cats, the protein’s immunoreactivity was also found in the epithelial cells of the straight testicular tubules and the epithelial cells of the rete testis.
  • The endothelial cells of capillaries, veins and lymphatic vessels were regularly found to be immunoreactive to the S-100 protein in ruminants (sheep, cattle).

Different Cellular Response

  • The Leydig cells (cells that produce testosterone) showed a strong positive response for S-100 protein in the testes of cats and rats, and to a lesser extent in pigs and horses.
  • A distinctive immunostaining of peritubular cells (cells around the seminiferous tubules) was confined to the testis of dogs and rats.

Implication and Hypothesis

  • The discrepancies of immunoreactivity for S-100 protein in various cell types of the testes across multiple species suggest the protein functions diversely.
  • The research supports the hypothesis that S-100 is a multifunctional protein and may have a distinctive function in the physiological operations of the testis depending on the species.

Cite This Article

APA
Amselgruber WM, Sinowatz F, Erhard M. (1994). Differential distribution of immunoreactive S-100 protein in mammalian testis. Histochemistry, 102(3), 241-245. https://doi.org/10.1007/BF00268901

Publication

Histochemistry
ISSN: 0301-5564
NlmUniqueID: 0411300
Country: Germany
Language: English
Volume: 102
Issue: 3
Pages: 241-245

Researcher Affiliations

Amselgruber, W M
  • Institute of Veterinary Anatomy, Histology and Embryology, University of Munich, Germany.
Sinowatz, F
    Erhard, M

      MeSH Terms

      • Animals
      • Cats
      • Cattle
      • Dogs
      • Horses
      • Immunohistochemistry
      • Male
      • Rats
      • S100 Proteins / analysis
      • Sheep
      • Swine
      • Testis / chemistry

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      Citations

      This article has been cited 1 times.
      1. Abd-Elmaksoud A, Shoeib MB, Marei HE. Localization of S-100 proteins in the testis and epididymis of poultry and rabbits.. Anat Cell Biol 2014 Sep;47(3):180-7.
        doi: 10.5115/acb.2014.47.3.180pubmed: 25276477google scholar: lookup
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