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Home / Equine Research Bank / J Anat / Discontinuities in the endothelium of epiphyseal cartilage c...
Journal of anatomy2015; 228(1); 162-175; doi: 10.1111/joa.12391

Discontinuities in the endothelium of epiphyseal cartilage canals and relevance to joint disease in foals.

Abstract: Cartilage canals have been shown to contain discontinuous blood vessels that enable circulating bacteria to bind to cartilage matrix, leading to vascular occlusion and associated pathological changes in pigs and chickens. It is also inconsistently reported that cartilage canals are surrounded by a cellular or acellular wall that may influence whether bacterial binding can occur. It is not known whether equine cartilage canals contain discontinuous endothelium or are surrounded by a wall. This study aimed to examine whether there were discontinuities in the endothelium of cartilage canal vessels, and whether canals had a cellular or acellular wall, in the epiphyseal growth cartilage of foals. Epiphyseal growth cartilage from the proximal third of the medial trochlear ridge of the distal femur from six healthy foals that were 1, 24, 35, 47, 118 and 122 days old and of different breeds and sexes was examined by light microscopy (LM), transmission electron microscopy (TEM) and immunohistochemistry. The majority of patent cartilage canals contained blood vessels that were lined by a thin layer of continuous endothelium. Fenestrations were found in two locations in one venule in a patent cartilage canal located deep in the growth cartilage and close to the ossification front in the 118-day-old foal. Chondrifying cartilage canals in all TEM-examined foals contained degenerated endothelial cells that were detached from the basement membrane, resulting in gap formation. Thirty-three percent of all canals were surrounded by a hypercellular rim that was interpreted as contribution of chondrocytes to growth cartilage. On LM, 69% of all cartilage canals were surrounded by a ring of matrix that stained intensely eosinophilic and consisted of collagen fibres on TEM that were confirmed to be collagen type I by immunohistochemistry. In summary, two types of discontinuity were observed in the endothelium of equine epiphyseal cartilage canal vessels: fenestrations were observed in a patent cartilage canal in the 118-day-old foal; and gaps were observed in chondrifying cartilage canals in all TEM-examined foals. Canals were not surrounded by any cellular wall, but a large proportion was surrounded by an acellular wall consisting of collagen type I. Bacterial binding can therefore probably occur in horses by mechanisms that are similar to those previously demonstrated in pigs and chickens.
© 2015 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of the Anatomical Society.
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Publication Date: 2015-10-15 PubMed ID: 26471892PubMed Central: PMC4694163DOI: 10.1111/joa.12391Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the structure of cartilage canals in young horses, specifically looking at the endothelium of these canals and how they might relate to bacterial infections leading to joint diseases. It was found that some of these canals are discontinuous, allowing bacteria to potentially bind to them, similar to observations in pigs and chickens.

Study Purpose and Methodology

  • The objective of the study was to investigate the endothelium of cartilage canals in the growth cartilage of foals. This exploration was guided by the assumption that these canals may have discontinuous endothelium and whether these canals are surrounded by a cellular or acellular wall.
  • The research analyzed the epiphyseal growth cartilage from six healthy foals of different breeds and sexes with varying ages, ranging from 1 to 122 days. The medium of study included light microscopy, transmission electron microscopy, and immunohistochemistry.

Findings

  • The majority of patent cartilage canals contained blood vessels that were lined by a thin layer of continuous endothelium.
  • On deeper examination, some discontinuity in the endothelium of these canals was found. This was noticed at two locations in a venule in one of the older foals (118 days old). This cartilage canal was located deep in the growth cartilage, close to the ossification front, an area where bone formation is taking place.
  • In addition, cartilage canals undergoing a process called chondrification were found to contain degenerated endothelial cells that have detached from the basement membrane, leaving gaps.
  • About 33% of canals had a hypercellular rim surrounding them, which was interpreted as a solution of chondrocytes to cartilage growth.
  • Visually, almost 70% of all cartilage canals were surrounded by a ring of matrix that stained intensely eosinophilic and contained collagen fibers. Further tests confirmed these to be collagen type I.

Implications

  • The study found two types of discontinuity in the endothelium of equine epiphyseal cartilage canal vessels.
  • It was also found that canals were not surrounded by any cellular wall, but a substantial number were surrounded by an acellular wall consisting primarily of collagen type I.
  • These findings suggest the potential for bacterial binding in these canals, akin to mechanisms previously demonstrated in pigs and chickens. This means that the breach in the endothelium may enable circulating bacteria to attach to cartilage matrix, which could lead to vascular occlusion and associated pathological changes.

Conclusion

  • The understanding of these structural details of cartilage canals could boost knowledge related to joint diseases in foals, particularly those associated with bacterial infections.
  • More research is necessary to definitively establish the correlation between these discontinuous canals and susceptibility to bacterial binding and subsequent joint diseases.

Cite This Article

APA
Hellings IR, Ekman S, Hultenby K, Dolvik NI, Olstad K. (2015). Discontinuities in the endothelium of epiphyseal cartilage canals and relevance to joint disease in foals. J Anat, 228(1), 162-175. https://doi.org/10.1111/joa.12391

Publication

Journal of anatomy
ISSN: 1469-7580
NlmUniqueID: 0137162
Country: England
Language: English
Volume: 228
Issue: 1
Pages: 162-175

Researcher Affiliations

Hellings, Ingunn Risnes
  • Department of Companion Animal Clinical Sciences, Equine Section, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, Oslo, Norway.
Ekman, Stina
  • Department of Biomedical Sciences and Veterinary Public Health, Section of Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Hultenby, Kjell
  • Department of Laboratory Medicine, Karolinska Institutet and University Hospital, Huddinge, Sweden.
Dolvik, Nils Ivar
  • Department of Companion Animal Clinical Sciences, Equine Section, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, Oslo, Norway.
Olstad, Kristin
  • Department of Companion Animal Clinical Sciences, Equine Section, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, Oslo, Norway.

MeSH Terms

  • Animals
  • Animals, Newborn
  • Cartilage, Articular / anatomy & histology
  • Cartilage, Articular / blood supply
  • Collagen Type I / analysis
  • Endothelium / anatomy & histology
  • Femur Head / anatomy & histology
  • Growth Plate / anatomy & histology
  • Growth Plate / blood supply
  • Horse Diseases / pathology
  • Horses / anatomy & histology
  • Immunohistochemistry
  • Joint Diseases / pathology
  • Microscopy, Electron
  • Regional Blood Flow

References

This article includes 57 references
  1. Alderson M, Speers D, Emslie K, Nade S. Acute haematogenous osteomyelitis and septic arthritis--a single disease. An hypothesis based upon the presence of transphyseal blood vessels.. J Bone Joint Surg Br 1986 Mar;68(2):268-74.
    pubmed: 3958014doi: 10.1302/0301-620x.68b2.3958014google scholar: lookup
  2. Alvarez J, Costales L, Serra R, Balbín M, López JM. Expression patterns of matrix metalloproteinases and vascular endothelial growth factor during epiphyseal ossification.. J Bone Miner Res 2005 Jun;20(6):1011-21.
    pubmed: 15883642doi: 10.1359/jbmr.050204google scholar: lookup
  3. Banks WJ (1993) Applied Veterinary Histology. St Louis, Missouri, USA: Mosby‐Year Book.
  4. Blumer MJ, Fritsch H, Pfaller K, Brenner E. Cartilage canals in the chicken embryo: ultrastructure and function.. Anat Embryol (Berl) 2004 Mar;207(6):453-62.
    pubmed: 14760531doi: 10.1007/s00429-003-0363-0google scholar: lookup
  5. Blumer MJ, Longato S, Fritsch H. Cartilage canals in the chicken embryo are involved in the process of endochondral bone formation within the epiphyseal growth plate.. Anat Rec A Discov Mol Cell Evol Biol 2004 Jul;279(1):692-700.
    pubmed: 15224411doi: 10.1002/ar.a.20058google scholar: lookup
  6. Blumer MJ, Longato S, Richter E, Pérez MT, Konakci KZ, Fritsch H. The role of cartilage canals in endochondral and perichondral bone formation: are there similarities between these two processes?. J Anat 2005 Apr;206(4):359-72.
    pmc: PMC1571487pubmed: 15817104doi: 10.1111/j.1469-7580.2005.00404.xgoogle scholar: lookup
  7. Blumer MJ, Schwarzer C, Pérez MT, Konakci KZ, Fritsch H. Identification and location of bone-forming cells within cartilage canals on their course into the secondary ossification centre.. J Anat 2006 Jun;208(6):695-707.
    pmc: PMC2100231pubmed: 16761972doi: 10.1111/j.1469-7580.2006.00578.xgoogle scholar: lookup
  8. Blumer MJ, Longato S, Schwarzer C, Fritsch H. Bone development in the femoral epiphysis of mice: the role of cartilage canals and the fate of resting chondrocytes.. Dev Dyn 2007 Aug;236(8):2077-88.
    pubmed: 17626280doi: 10.1002/dvdy.21228google scholar: lookup
  9. Blumer MJ, Longato S, Fritsch H. Structure, formation and role of cartilage canals in the developing bone.. Ann Anat 2008;190(4):305-15.
    pubmed: 18602255doi: 10.1016/j.aanat.2008.02.004google scholar: lookup
  10. Carlevaro MF, Cermelli S, Cancedda R, Descalzi Cancedda F. Vascular endothelial growth factor (VEGF) in cartilage neovascularization and chondrocyte differentiation: auto-paracrine role during endochondral bone formation.. J Cell Sci 2000 Jan;113 ( Pt 1):59-69.
    pubmed: 10591625doi: 10.1242/jcs.113.1.59google scholar: lookup
  11. Carlson CS, Hilley HD, Henrikson CK. Ultrastructure of normal epiphyseal cartilage of the articular-epiphyseal cartilage complex in growing swine.. Am J Vet Res 1985 Feb;46(2):306-13.
    pubmed: 3994097
  12. Carlson CS, Hilley HD, Meuten DJ. Degeneration of cartilage canal vessels associated with lesions of osteochondrosis in swine.. Vet Pathol 1989 Jan;26(1):47-54.
    pubmed: 2913703doi: 10.1177/030098588902600108google scholar: lookup
  13. Carlson CS, Meuten DJ, Richardson DC. Ischemic necrosis of cartilage in spontaneous and experimental lesions of osteochondrosis.. J Orthop Res 1991 May;9(3):317-29.
    pubmed: 2010836doi: 10.1002/jor.1100090303google scholar: lookup
  14. Carlson CS, Cullins LD, Meuten DJ. Osteochondrosis of the articular-epiphyseal cartilage complex in young horses: evidence for a defect in cartilage canal blood supply.. Vet Pathol 1995 Nov;32(6):641-7.
    pubmed: 8592799doi: 10.1177/030098589503200605google scholar: lookup
  15. Chagnot C, Listrat A, Astruc T, Desvaux M. Bacterial adhesion to animal tissues: protein determinants for recognition of extracellular matrix components.. Cell Microbiol 2012 Nov;14(11):1687-96.
    pubmed: 22882798doi: 10.1111/cmi.12002google scholar: lookup
  16. Cogger VC, Roessner U, Warren A, Fraser R, Le Couteur DG. A Sieve-Raft Hypothesis for the regulation of endothelial fenestrations.. Comput Struct Biotechnol J 2013;8:e201308003.
    pmc: PMC3962122pubmed: 24688743doi: 10.5936/csbj.201308003google scholar: lookup
  17. Denecke R, Trautwein G. Articular cartilage canals--a new pathogenetic mechanism in infectious arthritis.. Experientia 1986 Sep 15;42(9):999-1001.
    pubmed: 3758304doi: 10.1007/bf01940704google scholar: lookup
  18. Denecke R, Trautwein G, Kaup FJ. The role of cartilage canals in the pathogenesis of experimentally induced polyarthritis.. Rheumatol Int 1986;6(6):239-43.
    pubmed: 3809886doi: 10.1007/bf00541313google scholar: lookup
  19. Doménech‐Ratto G, Fernández‐Villacañas Márin M, Ballester‐Moreno A. Development and segments of cartilage canals in the chick embryo. A light microscope study.. Eur J Anat 3, 121–126.
  20. Edwards AM, Massey RC. How does Staphylococcus aureus escape the bloodstream?. Trends Microbiol 2011 Apr;19(4):184-90.
    pubmed: 21227700doi: 10.1016/j.tim.2010.12.005google scholar: lookup
  21. Ekman S, Heinegård D. Immunohistochemical localization of matrix proteins in the femoral joint cartilage of growing commercial pigs.. Vet Pathol 1992 Nov;29(6):514-20.
    pubmed: 1448898doi: 10.1177/030098589202900605google scholar: lookup
  22. Ekman S, Rodriguez-Martinez H, Plöen L. Morphology of normal and osteochondrotic porcine articular-epiphyseal cartilage. A study in the domestic pig and minipig of wild hog ancestry.. Acta Anat (Basel) 1990;139(3):239-53.
    pubmed: 2077805
  23. Emslie KR, Nade S. Acute hematogenous staphylococcal osteomyelitis. A description of the natural history in an avian model.. Am J Pathol 1983 Mar;110(3):333-45.
    pmc: PMC1916166pubmed: 6829712
  24. Ganey TM, Ogden JA, Sasse J, Neame PJ, Hilbelink DR. Basement membrane composition of cartilage canals during development and ossification of the epiphysis.. Anat Rec 1995 Mar;241(3):425-37.
    pubmed: 7755183doi: 10.1002/ar.1092410318google scholar: lookup
  25. Grøndahl AM, Dolvik NI. Heritability estimations of osteochondrosis in the tibiotarsal joint and of bony fragments in the palmar/plantar portion of the metacarpo- and metatarsophalangeal joints of horses.. J Am Vet Med Assoc 1993 Jul 1;203(1):101-4.
    pubmed: 8407439
  26. Haggett EF, Foote AK, Head MJ, McGladdery AJ, Powell SE. Necrosis of the femoral condyles in a four-week-old foal: clinical, imaging and histopathological features.. Equine Vet J Suppl 2012 Feb;(41):91-5.
    pubmed: 22594034doi: 10.1111/j.2042-3306.2011.00498.xgoogle scholar: lookup
  27. Haines RW. The pseudoepiphysis of the first metacarpal of man.. J Anat 1974 Feb;117(Pt 1):145-58.
    pmc: PMC1231440pubmed: 4844656
  28. Hance SR, Schneider RK, Embertson RM, Bramlage LR, Wicks JR. Lesions of the caudal aspect of the femoral condyles in foals: 20 cases (1980-1990).. J Am Vet Med Assoc 1993 Feb 15;202(4):637-46.
    pubmed: 8449810
  29. Hayashi K. Three-dimensional organization of the cartilage canal--a scanning electron-microscopic study by vascular cast of the rabbit's femoral head.. Nihon Seikeigeka Gakkai Zasshi 1992 May;66(5):548-59.
    pubmed: 1506750
  30. Hedberg A, Messner K, Persliden J, Hildebrand C. Transient local presence of nerve fibers at onset of secondary ossification in the rat knee joint.. Anat Embryol (Berl) 1995 Sep;192(3):247-55.
    pubmed: 8651509doi: 10.1007/bf00184749google scholar: lookup
  31. Hendrickson EHS, Lykkjen S, Dolvik NI. Radiographic osteochondrosis and osteochondral fragmentation in horses treated for infection prior to 6 months of age.. Manuscript submitted for publication.
  32. Henson FMD, Davies ME, Schofield PN, Jeffcott LB. Expression of types II, VI and X collagen in equine growth cartilage during development.. Equine Vet J 1996 May;28(3):189-198.
    pubmed: 28976712doi: 10.1111/j.2042-3306.1996.tb03772.xgoogle scholar: lookup
  33. Howlett CR. The fine structure of the proximal growth plate and metaphysis of the avian tibia: endochondral osteogenesis.. J Anat 1980 Jun;130(Pt 4):745-68.
    pmc: PMC1233199pubmed: 6159341
  34. Hunt CD, Ollerich DA, Nielsen FH. Morphology of the perforating cartilage canals in the proximal tibial growth plate of the chick.. Anat Rec 1979 May;194(1):143-57.
    pubmed: 443560doi: 10.1002/ar.1091940110google scholar: lookup
  35. Kugler JH, Tomlinson A, Wagstaff A, Ward SM. The role of cartilage canals in the formation of secondary centres of ossification.. J Anat 1979 Oct;129(Pt 3):493-506.
    pmc: PMC1233016pubmed: 541238
  36. Laverty S, Girard C. Pathogenesis of epiphyseal osteochondrosis.. Vet J 2013 Jul;197(1):3-12.
    pubmed: 23647656doi: 10.1016/j.tvjl.2013.03.035google scholar: lookup
  37. Laverty S, Okouneff S, Ionescu M, Reiner A, Pidoux I, Webber C, Rossier Y, Billinghurst RC, Poole AR. Excessive degradation of type II collagen in articular cartilage in equine osteochondrosis.. J Orthop Res 2002 Nov;20(6):1282-9.
    pubmed: 12472241doi: 10.1016/s0736-0266(02)00053-0google scholar: lookup
  38. Lecocq M, Girard CA, Fogarty U, Beauchamp G, Richard H, Laverty S. Cartilage matrix changes in the developing epiphysis: early events on the pathway to equine osteochondrosis?. Equine Vet J 2008 Jul;40(5):442-54.
    pubmed: 18487100doi: 10.2746/042516408x297453google scholar: lookup
  39. van de Lest CH, Brama PA, van El B, DeGroot J, van Weeren PR. Extracellular matrix changes in early osteochondrotic defects in foals: a key role for collagen?. Biochim Biophys Acta 2004 Sep 6;1690(1):54-62.
    pubmed: 15337170doi: 10.1016/j.bbadis.2004.05.002google scholar: lookup
  40. Lutfi AM. Mode of growth, fate and functions of cartilage canals.. J Anat 1970 Jan;106(Pt 1):135-45.
    pmc: PMC1233858pubmed: 5413561
  41. Lutfi AM. Study of cell multiplication in the cartilaginous upper end of the tibia of the domestic fowl by tritiated thymidine autoradiography.. Acta Anat (Basel) 1970;76(3):454-63.
    pubmed: 5492414doi: 10.1159/000143507google scholar: lookup
  42. McIlwraith CW. Inferences from referred clinical cases of osteochondritis dissecans.. Equine Vet J Suppl 16, 27–30.
  43. Olstad K, Ytrehus B, Ekman S, Carlson CS, Dolvik NI. Early lesions of osteochondrosis in the distal tibia of foals.. J Orthop Res 2007 Aug;25(8):1094-105.
    pubmed: 17415757doi: 10.1002/jor.20375google scholar: lookup
  44. Olstad K, Cnudde V, Masschaele B, Thomassen R, Dolvik NI. Micro-computed tomography of early lesions of osteochondrosis in the tarsus of foals.. Bone 2008 Sep;43(3):574-83.
    pubmed: 18579463doi: 10.1016/j.bone.2008.04.024google scholar: lookup
  45. Olstad K, Ytrehus B, Ekman S, Carlson CS, Dolvik NI. Epiphyseal cartilage canal blood supply to the tarsus of foals and relationship to osteochondrosis.. Equine Vet J 2008 Jan;40(1):30-9.
    pubmed: 18083657doi: 10.2746/042516407x239836google scholar: lookup
  46. Olstad K, Ytrehus B, Ekman S, Carlson CS, Dolvik NI. Epiphyseal cartilage canal blood supply to the metatarsophalangeal joint of foals.. Equine Vet J 2009 Dec;41(9):865-71.
    pubmed: 20383983doi: 10.2746/042516409x437762google scholar: lookup
  47. Olstad K, Hendrickson EH, Carlson CS, Ekman S, Dolvik NI. Transection of vessels in epiphyseal cartilage canals leads to osteochondrosis and osteochondrosis dissecans in the femoro-patellar joint of foals; a potential model of juvenile osteochondritis dissecans.. Osteoarthritis Cartilage 2013 May;21(5):730-8.
    pubmed: 23428601doi: 10.1016/j.joca.2013.02.005google scholar: lookup
  48. Reiland S, Ordell N, Lundeheim N, Olsson SE. Heredity of osteochondrosis, body constitution and leg weakness in the pig. A correlative investigation using progeny testing.. Acta Radiol Suppl 1978;358:123-37.
    pubmed: 233592
  49. Semevolos SA, Nixon AJ, Brower-Toland BD. Changes in molecular expression of aggrecan and collagen types I, II, and X, insulin-like growth factor-I, and transforming growth factor-beta1 in articular cartilage obtained from horses with naturally acquired osteochondrosis.. Am J Vet Res 2001 Jul;62(7):1088-94.
    pubmed: 11453485doi: 10.2460/ajvr.2001.62.1088google scholar: lookup
  50. Speers DJ, Nade SM. Ultrastructural studies of adherence of Staphylococcus aureus in experimental acute hematogenous osteomyelitis.. Infect Immun 1985 Aug;49(2):443-6.
    pmc: PMC262038pubmed: 4018875doi: 10.1128/iai.49.2.443-446.1985google scholar: lookup
  51. Stockwell RA. The ultrastructure of cartilage canals and the surrounding cartilage in the sheep fetus.. J Anat 1971 Sep;109(Pt 3):397-410.
    pmc: PMC1270983pubmed: 4949287
  52. Trueta J. The three types of acute haematogenous osteomyelitis.. J Bone Joint Surg Br 41B, 671–680.
  53. Wilsman NJ, Van Sickle DC. Cartilage canals, their morphology and distribution.. Anat Rec 1972 May;173(1):79-93.
    pubmed: 5028066doi: 10.1002/ar.1091730107google scholar: lookup
  54. Woodard JC, Becker HN, Poulos PW Jr. Articular cartilage blood vessels in swine osteochondrosis.. Vet Pathol 1987 Mar;24(2):118-23.
    pubmed: 3576906doi: 10.1177/030098588702400203google scholar: lookup
  55. Ytrehus B, Carlson CS, Lundeheim N, Mathisen L, Reinholt FP, Teige J, Ekman S. Vascularisation and osteochondrosis of the epiphyseal growth cartilage of the distal femur in pigs--development with age, growth rate, weight and joint shape.. Bone 2004 Mar;34(3):454-65.
    pubmed: 15003793doi: 10.1016/j.bone.2003.07.011google scholar: lookup
  56. Ytrehus B, Ekman S, Carlson CS, Teige J, Reinholt FP. Focal changes in blood supply during normal epiphyseal growth are central in the pathogenesis of osteochondrosis in pigs.. Bone 2004 Dec;35(6):1294-306.
    pubmed: 15589210doi: 10.1016/j.bone.2004.08.016google scholar: lookup
  57. Ytrehus B, Carlson CS, Ekman S. Etiology and pathogenesis of osteochondrosis.. Vet Pathol 2007 Jul;44(4):429-48.
    pubmed: 17606505doi: 10.1354/vp.44-4-429google scholar: lookup

Citations

This article has been cited 4 times.
  1. Olstad K, Aasmundstad T, Kongsro J, Grindflek E. Osteochondrosis and other lesions in all intervertebral, articular process and rib joints from occiput to sacrum in pigs with poor back conformation, and relationship to juvenile kyphosis. BMC Vet Res 2022 Jan 18;18(1):44.
    doi: 10.1186/s12917-021-03091-6pubmed: 35042517google scholar: lookup
  2. Hendrickson EHS, Lykkjen S, Dolvik NI, Olstad K. Prevalence of osteochondral lesions in the fetlock and hock joints of Standardbred horses that survived bacterial infection before 6 months of age. BMC Vet Res 2018 Dec 10;14(1):390.
    doi: 10.1186/s12917-018-1726-3pubmed: 30526583google scholar: lookup
  3. Finnøy A, Olstad K, Lilledahl MB. Non-linear optical microscopy of cartilage canals in the distal femur of young pigs may reveal the cause of articular osteochondrosis. BMC Vet Res 2017 Aug 22;13(1):270.
    doi: 10.1186/s12917-017-1197-ypubmed: 28830435google scholar: lookup
  4. Hellings IR, Dolvik NI, Ekman S, Olstad K. Cartilage canals in the distal intermediate ridge of the tibia of fetuses and foals are surrounded by different types of collagen. J Anat 2017 Oct;231(4):615-625.
    doi: 10.1111/joa.12650pubmed: 28620929google scholar: lookup
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