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Home / Equine Research Bank / J Appl Genet / Distribution of coat-color-associated alleles in the domesti...
Journal of applied genetics2016; 57(4); 519-525; doi: 10.1007/s13353-016-0352-7

Distribution of coat-color-associated alleles in the domestic horse population and Przewalski’s horse.

Abstract: Considering the hidden mode of inheritance of some coat-color-associated alleles, we investigated the presence/absence of coat-color-associated alleles in 1093 domestic horses of 55 breeds and 20 specimens of Przewalski's horse. For coat-color genotyping, allele specific PCR, pyrosequencing and Li-Cor analyses were conducted on 12 coat-color-associated alleles of five genes. Our data provide deep insight into the distribution of coat-color-associated alleles within breeds. We found that the alleles for the basic colorations (bay, black, and chestnut) are widely distributed and occur in nearly all breeds. Alleles leading to dilutions or patterns are rare in domestic breeds and were not found in Przewalski's horse. Higher frequencies of these alleles are only found in breeds that are selected for their expressed phenotypes (e.g., Kinsky horse, Lewitzer, Tinker). Nevertheless, our study produced strong evidence that molecular testing of the coat color is necessary for well-defined phenotyping to avoid unexpected colorations of offspring that can result in legal action.
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Publication Date: 2016-05-18 PubMed ID: 27194311DOI: 10.1007/s13353-016-0352-7Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the distribution of different genes associated with coat color in domestic horses and Przewalski’s horses. The researchers used advanced genetic testing methods on 1093 domestic horses and 20 Przewalski’s horses, providing a comprehensive view of the common and rare coat color patterns among these species.

Research Methods

  • The research involved 1093 domestic horses from 55 different breeds and 20 specimens of Przewalski’s horse, the only truly wild horse species left in the world.
  • Advanced genetic testing techniques were used to identify 12 coat-color-associated alleles in five different genes. These techniques included allele-specific PCR, a test that amplifies and identifies specific gene sequences, pyrosequencing, a method for identifying precise genetic sequences, and Li-Cor analyses, another genetic testing method.

Findings

  • The researchers discovered that alleles, variations of a gene, associated with basic colors like bay, black and chestnut are widespread across all breeds.
  • Contrastingly, alleles that result in different shades or patterns are infrequent in domestic horse breeds and non-existent in Przewalski’s horse.
  • However, particular horse breeds that have been genetically selected for their distinctive coat colorations, such as the Kinsky horse, Lewitzer and Tinker, exhibited higher frequencies of these unusual color-associated alleles.

Significance of the Study

  • The study provides new insights into the genetic basis of coat color in horses and underscores the wide distribution of basic color alleles across all breeds.
  • More importantly, it establishes the necessity of molecular testing for a well-defined phenotyping of horses’ coat color. This is crucial, as surprising colorations in offspring may lead to legal issues, presumably in cases of valuable breed horses where a specific color might be expected or guaranteed.

Cite This Article

APA
Reissmann M, Musa L, Zakizadeh S, Ludwig A. (2016). Distribution of coat-color-associated alleles in the domestic horse population and Przewalski’s horse. J Appl Genet, 57(4), 519-525. https://doi.org/10.1007/s13353-016-0352-7

Publication

Journal of applied genetics
ISSN: 2190-3883
NlmUniqueID: 9514582
Country: England
Language: English
Volume: 57
Issue: 4
Pages: 519-525

Researcher Affiliations

Reissmann, Monika
  • Albrecht Daniel Thaer Institute, Humboldt University Berlin, Berlin, Germany.
Musa, Lutfi
  • Department of Genetics and Animal Breeding, Faculty of Animal Production, University of Khartoum, Khartoum, Sudan.
Zakizadeh, Sonia
  • Department of Animal Science, Jihad-Agricultural Higher Educational Complex, Mashad, Iran.
Ludwig, Arne
  • Department of Evolutionary Genetics, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany. ludwig@izw-berlin.de.

MeSH Terms

  • Alleles
  • Animals
  • Breeding
  • Genetics, Population
  • Genotyping Techniques
  • Hair
  • Horses / classification
  • Horses / genetics
  • Phenotype
  • Pigmentation / genetics

References

This article includes 36 references
  1. Haase B, Rieder S, Tozaki T, Hasegawa T, Penedo MC, Jude R, Leeb T. Five novel KIT mutations in horses with white coat colour phenotypes.. Anim Genet 2011 Jun;42(3):337-9.
    pubmed: 21554354doi: 10.1111/j.1365-2052.2011.02173.xgoogle scholar: lookup
  2. Lindgren G, Backström N, Swinburne J, Hellborg L, Einarsson A, Sandberg K, Cothran G, Vilà C, Binns M, Ellegren H. Limited number of patrilines in horse domestication.. Nat Genet 2004 Apr;36(4):335-6.
    pubmed: 15034578doi: 10.1038/ng1326google scholar: lookup
  3. Jansen T, Forster P, Levine MA, Oelke H, Hurles M, Renfrew C, Weber J, Olek K. Mitochondrial DNA and the origins of the domestic horse.. Proc Natl Acad Sci U S A 2002 Aug 6;99(16):10905-10.
    pubmed: 12130666doi: 10.1073/pnas.152330099google scholar: lookup
  4. Kakoi H, Tozaki T, Nagata S, Gawahara H, Kijima-Suda I. Development of a method for simultaneously genotyping multiple horse coat colour loci and genetic investigation of basic colour variation in Thoroughbred and Misaki horses in Japan.. J Anim Breed Genet 2009 Dec;126(6):425-31.
    pubmed: 19912416doi: 10.1111/j.1439-0388.2009.00841.xgoogle scholar: lookup
  5. Andersson LS, Axelsson J, Dubielzig RR, Lindgren G, Ekesten B. Multiple congenital ocular anomalies in Icelandic horses.. BMC Vet Res 2011 May 26;7:21.
    pubmed: 21615885doi: 10.1186/1746-6148-7-21google scholar: lookup
  6. Bai DY, Yang LH, Unerhu U, Zhao YP, Zhao QN, Hasigaowa H, Dugarjaviin M. [Effects of Kit gene on coat depigmentation in white horses].. Yi Chuan 2011 Nov;33(11):1171-8.
    pubmed: 22120071doi: 10.3724/sp.j.1005.2011.01171google scholar: lookup
  7. Wallner B, Vogl C, Shukla P, Burgstaller JP, Druml T, Brem G. Identification of genetic variation on the horse y chromosome and the tracing of male founder lineages in modern breeds.. PLoS One 2013;8(4):e60015.
    pubmed: 23573227doi: 10.1371/journal.pone.0060015google scholar: lookup
  8. Warmuth V, Eriksson A, Bower MA, Barker G, Barrett E, Hanks BK, Li S, Lomitashvili D, Ochir-Goryaeva M, Sizonov GV, Soyonov V, Manica A. Reconstructing the origin and spread of horse domestication in the Eurasian steppe.. Proc Natl Acad Sci U S A 2012 May 22;109(21):8202-6.
    pubmed: 22566639doi: 10.1073/pnas.1111122109google scholar: lookup
  9. Cieslak M, Pruvost M, Benecke N, Hofreiter M, Morales A, Reissmann M, Ludwig A. Origin and history of mitochondrial DNA lineages in domestic horses.. PLoS One 2010 Dec 20;5(12):e15311.
    pubmed: 21187961doi: 10.1371/journal.pone.0015311google scholar: lookup
  10. Marklund L, Johansson Moller M, Høyheim B, Davies W, Fredholm M, Juneja RK, Mariani P, Coppieters W, Ellegren H, Andersson L. A comprehensive linkage map of the pig based on a wild pig-Large White intercross.. Anim Genet 1996 Aug;27(4):255-69.
    pubmed: 8856923doi: 10.1111/j.1365-2052.1996.tb00487.xgoogle scholar: lookup
  11. Lippold S, Knapp M, Kuznetsova T, Leonard JA, Benecke N, Ludwig A, Rasmussen M, Cooper A, Weinstock J, Willerslev E, Shapiro B, Hofreiter M. Discovery of lost diversity of paternal horse lineages using ancient DNA.. Nat Commun 2011 Aug 23;2:450.
    pubmed: 21863017doi: 10.1038/ncomms1447google scholar: lookup
  12. Hauswirth R, Jude R, Haase B, Bellone RR, Archer S, Holl H, Brooks SA, Tozaki T, Penedo MC, Rieder S, Leeb T. Novel variants in the KIT and PAX3 genes in horses with white-spotted coat colour phenotypes.. Anim Genet 2013 Dec;44(6):763-5.
    pubmed: 23659293doi: 10.1111/age.12057google scholar: lookup
  13. Haase B, Jagannathan V, Rieder S, Leeb T. A novel KIT variant in an Icelandic horse with white-spotted coat colour.. Anim Genet 2015 Aug;46(4):466.
    pubmed: 26059442doi: 10.1111/age.12313google scholar: lookup
  14. Reissmann M, Ludwig A. Pleiotropic effects of coat colour-associated mutations in humans, mice and other mammals.. Semin Cell Dev Biol 2013 Jun-Jul;24(6-7):576-86.
    pubmed: 23583561doi: 10.1016/j.semcdb.2013.03.014google scholar: lookup
  15. Ling Y, Ma Y, Guan W, Cheng Y, Wang Y, Han J, Jin D, Mang L, Mahmut H. Identification of Y chromosome genetic variations in Chinese indigenous horse breeds.. J Hered 2010 Sep-Oct;101(5):639-43.
    pubmed: 20497969doi: 10.1093/jhered/esq047google scholar: lookup
  16. Andersson LS, Lyberg K, Cothran G, Ramsey DT, Juras R, Mikko S, Ekesten B, Ewart S, Lindgren G. Targeted analysis of four breeds narrows equine Multiple Congenital Ocular Anomalies locus to 208 kilobases.. Mamm Genome 2011 Jun;22(5-6):353-60.
    pubmed: 21465164doi: 10.1007/s00335-011-9325-7google scholar: lookup
  17. Andersson LS, Juras R, Ramsey DT, Eason-Butler J, Ewart S, Cothran G, Lindgren G. Equine Multiple Congenital Ocular Anomalies maps to a 4.9 megabase interval on horse chromosome 6.. BMC Genet 2008 Dec 19;9:88.
    pubmed: 19099555doi: 10.1186/1471-2156-9-88google scholar: lookup
  18. Pruvost M, Reissmann M, Benecke N, Ludwig A. From genes to phenotypes - evaluation of two methods for the SNP analysis in archaeological remains: pyrosequencing and competitive allele specific PCR (KASPar).. Ann Anat 2012 Jan 20;194(1):74-81.
    pubmed: 22154270doi: 10.1016/j.aanat.2011.10.007google scholar: lookup
  19. Brooks SA, Bailey E. Exon skipping in the KIT gene causes a Sabino spotting pattern in horses.. Mamm Genome 2005 Nov;16(11):893-902.
    pubmed: 16284805doi: 10.1007/s00335-005-2472-ygoogle scholar: lookup
  20. Komáromy AM, Rowlan JS, La Croix NC, Mangan BG. Equine Multiple Congenital Ocular Anomalies (MCOA) syndrome in PMEL17 (Silver) mutant ponies: five cases.. Vet Ophthalmol 2011 Sep;14(5):313-20.
    pubmed: 21929608doi: 10.1111/j.1463-5224.2011.00878.xgoogle scholar: lookup
  21. Campana MG, Whitten CM, Edwards CJ, Stock F, Murphy AM, Binns MM, Barker GW, Bower MA. Accurate determination of phenotypic information from historic thoroughbred horses by single base extension.. PLoS One 2010 Dec 2;5(12):e15172.
    pubmed: 21152043doi: 10.1371/journal.pone.0015172google scholar: lookup
  22. Rieder S. Molecular tests for coat colours in horses.. J Anim Breed Genet 2009 Dec;126(6):415-24.
    pubmed: 19912415doi: 10.1111/j.1439-0388.2009.00832.xgoogle scholar: lookup
  23. Ludwig A, Pruvost M, Reissmann M, Benecke N, Brockmann GA, Castaños P, Cieslak M, Lippold S, Llorente L, Malaspinas AS, Slatkin M, Hofreiter M. Coat color variation at the beginning of horse domestication.. Science 2009 Apr 24;324(5926):485.
    pubmed: 19390039doi: 10.1126/science.1172750google scholar: lookup
  24. Kavar T, Čeh E, Dovč P. A simplified PCR-based method for detection of gray coat color allele in horse.. Mol Cell Probes 2012 Dec;26(6):256-8.
    pubmed: 22433981doi: 10.1016/j.mcp.2012.02.006google scholar: lookup
  25. Pruvost M, Bellone R, Benecke N, Sandoval-Castellanos E, Cieslak M, Kuznetsova T, Morales-Muñiz A, O'Connor T, Reissmann M, Hofreiter M, Ludwig A. Genotypes of predomestic horses match phenotypes painted in Paleolithic works of cave art.. Proc Natl Acad Sci U S A 2011 Nov 15;108(46):18626-30.
    pubmed: 22065780doi: 10.1073/pnas.1108982108google scholar: lookup
  26. Bellone RR, Holl H, Setaluri V, Devi S, Maddodi N, Archer S, Sandmeyer L, Ludwig A, Foerster D, Pruvost M, Reissmann M, Bortfeldt R, Adelson DL, Lim SL, Nelson J, Haase B, Engensteiner M, Leeb T, Forsyth G, Mienaltowski MJ, Mahadevan P, Hofreiter M, Paijmans JL, Gonzalez-Fortes G, Grahn B, Brooks SA. Evidence for a retroviral insertion in TRPM1 as the cause of congenital stationary night blindness and leopard complex spotting in the horse.. PLoS One 2013;8(10):e78280.
    pubmed: 24167615doi: 10.1371/journal.pone.0078280google scholar: lookup
  27. Haase B, Brooks SA, Tozaki T, Burger D, Poncet PA, Rieder S, Hasegawa T, Penedo C, Leeb T. Seven novel KIT mutations in horses with white coat colour phenotypes.. Anim Genet 2009 Oct;40(5):623-9.
    pubmed: 19456317doi: 10.1111/j.1365-2052.2009.01893.xgoogle scholar: lookup
  28. Achilli A, Olivieri A, Soares P, Lancioni H, Hooshiar Kashani B, Perego UA, Nergadze SG, Carossa V, Santagostino M, Capomaccio S, Felicetti M, Al-Achkar W, Penedo MC, Verini-Supplizi A, Houshmand M, Woodward SR, Semino O, Silvestrelli M, Giulotto E, Pereira L, Bandelt HJ, Torroni A. Mitochondrial genomes from modern horses reveal the major haplogroups that underwent domestication.. Proc Natl Acad Sci U S A 2012 Feb 14;109(7):2449-54.
    pubmed: 22308342doi: 10.1073/pnas.1111637109google scholar: lookup
  29. Der Sarkissian C, Ermini L, Schubert M, Yang MA, Librado P, Fumagalli M, Jónsson H, Bar-Gal GK, Albrechtsen A, Vieira FG, Petersen B, Ginolhac A, Seguin-Orlando A, Magnussen K, Fages A, Gamba C, Lorente-Galdos B, Polani S, Steiner C, Neuditschko M, Jagannathan V, Feh C, Greenblatt CL, Ludwig A, Abramson NI, Zimmermann W, Schafberg R, Tikhonov A, Sicheritz-Ponten T, Willerslev E, Marques-Bonet T, Ryder OA, McCue M, Rieder S, Leeb T, Slatkin M, Orlando L. Evolutionary Genomics and Conservation of the Endangered Przewalski's Horse.. Curr Biol 2015 Oct 5;25(19):2577-83.
    pubmed: 26412128doi: 10.1016/j.cub.2015.08.032google scholar: lookup
  30. Hauswirth R, Haase B, Blatter M, Brooks SA, Burger D, Drögemüller C, Gerber V, Henke D, Janda J, Jude R, Magdesian KG, Matthews JM, Poncet PA, Svansson V, Tozaki T, Wilkinson-White L, Penedo MC, Rieder S, Leeb T. Mutations in MITF and PAX3 cause "splashed white" and other white spotting phenotypes in horses.. PLoS Genet 2012;8(4):e1002653.
    pubmed: 22511888doi: 10.1371/journal.pgen.1002653google scholar: lookup
  31. Andersson LS, Wilbe M, Viluma A, Cothran G, Ekesten B, Ewart S, Lindgren G. Equine multiple congenital ocular anomalies and silver coat colour result from the pleiotropic effects of mutant PMEL.. PLoS One 2013;8(9):e75639.
    pubmed: 24086599doi: 10.1371/journal.pone.0075639google scholar: lookup
  32. Bellone RR. Pleiotropic effects of pigmentation genes in horses.. Anim Genet 2010 Dec;41 Suppl 2:100-10.
    pubmed: 21070283doi: 10.1111/j.1365-2052.2010.02116.xgoogle scholar: lookup
  33. Rieder S, Taourit S, Mariat D, Langlois B, Guérin G. Mutations in the agouti (ASIP), the extension (MC1R), and the brown (TYRP1) loci and their association to coat color phenotypes in horses (Equus caballus).. Mamm Genome 2001 Jun;12(6):450-5.
    pubmed: 11353392doi: 10.1007/s003350020017google scholar: lookup
  34. Wagner HJ, Reissmann M. New polymorphism detected in the horse MC1R gene.. Anim Genet 2000 Aug;31(4):289-90.
    pubmed: 11086549doi: 10.1046/j.1365-2052.2000.00655.xgoogle scholar: lookup
  35. Haase B, Brooks SA, Schlumbaum A, Azor PJ, Bailey E, Alaeddine F, Mevissen M, Burger D, Poncet PA, Rieder S, Leeb T. Allelic heterogeneity at the equine KIT locus in dominant white (W) horses.. PLoS Genet 2007 Nov;3(11):e195.
    pubmed: 17997609doi: 10.1371/journal.pgen.0030195google scholar: lookup
  36. Cieslak M, Reissmann M, Hofreiter M, Ludwig A. Colours of domestication.. Biol Rev Camb Philos Soc 2011 Nov;86(4):885-99.
    pubmed: 21443614doi: 10.1111/j.1469-185X.2011.00177.xgoogle scholar: lookup

Citations

This article has been cited 6 times.
  1. Musiał AD, Ropka-Molik K, Stefaniuk-Szmukier M, Myćka G, Bieniek A, Yasynetska N. Characteristic of Przewalski horses population from Askania-Nova reserve based on genetic markers.. Mol Biol Rep 2023 Aug;50(8):7121-7126.
    doi: 10.1007/s11033-023-08581-4pubmed: 37365410google scholar: lookup
  2. Avila F, Hughes SS, Magdesian KG, Penedo MCT, Bellone RR. Breed Distribution and Allele Frequencies of Base Coat Color, Dilution, and White Patterning Variants across 28 Horse Breeds.. Genes (Basel) 2022 Sep 13;13(9).
    doi: 10.3390/genes13091641pubmed: 36140807google scholar: lookup
  3. Marín Navas C, Delgado Bermejo JV, McLean AK, León Jurado JM, Torres ARBYR, Navas González FJ. One Hundred Years of Coat Colour Influences on Genetic Diversity in the Process of Development of a Composite Horse Breed.. Vet Sci 2022 Feb 6;9(2).
    doi: 10.3390/vetsci9020068pubmed: 35202321google scholar: lookup
  4. Voß K, Tetens J, Thaller G, Becker D. Coat Color Roan Shows Association with KIT Variants and No Evidence of Lethality in Icelandic Horses.. Genes (Basel) 2020 Jun 22;11(6).
    doi: 10.3390/genes11060680pubmed: 32580410google scholar: lookup
  5. Reissmann M, Lutz W, Lieckfeldt D, Sandoval-Castellanos E, Ludwig A. An Agouti-Signaling-Protein Mutation is Strongly Associated with Melanism in European Roe Deer (Capreolus capreolus).. Genes (Basel) 2020 Jun 11;11(6).
    doi: 10.3390/genes11060647pubmed: 32545389google scholar: lookup
  6. Druml T, Grilz-Seger G, Neuditschko M, Horna M, Ricard A, Pausch H, Brem G. Novel insights into Sabino1 and splashed white coat color patterns in horses.. Anim Genet 2018 Jun;49(3):249-253.
    doi: 10.1111/age.12657pubmed: 29635692google scholar: lookup
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