FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Equine Vet J / Distribution of equid herpesvirus-1 (EHV-1) in respiratory t...
Equine veterinary journal1994; 26(6); 470-473; doi: 10.1111/j.2042-3306.1994.tb04052.x

Distribution of equid herpesvirus-1 (EHV-1) in respiratory tract associated lymphoid tissue: implications for cellular immunity.

Abstract: Twelve adult ponies and 2 conventional foals were exposed intranasally to EHV-1, strain Ab4 (TCID50 10(-6.6) and samples of respiratory tract associated lymphoid tissues were recovered between 12 h and 13 days after infection. Infectious virus was detected in tissue homogenates using susceptible cell monolayers and expression of viral antigens was monitored using indirect immunoperoxidase histochemistry on paraffin sections. The results showed both infectious EHV-1 and viral antigens in respiratory tract associated lymph nodes 12 h after exposure. Infected leucocytes were identified morphologically as lymphocytes, monocytes, macrophages and plasma cells. The rapid intracellular localisation of EHV-1 in lymph nodes implies that cell mediated immunity is an important aspect of the equine response to this virus.
Get Full Text
Publication Date: 1994-11-01 PubMed ID: 7889921DOI: 10.1111/j.2042-3306.1994.tb04052.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the distribution of Equid Herpesvirus-1 (EHV-1), a common horse virus, in the respiratory tract-associated lymphoid tissue of ponies and foals, highlighting the importance of cell-mediated immunity in response to the virus.

Methodology

  • The study involved twelve adult ponies and two standard foals, all of which were exposed to EHV-1 via intranasal means.
  • The virus used to infect the subjects was of strain Ab4 (TCID50 10(-6.6)).
  • Respiratory tract associated lymphoid tissue samples were collected from the subjects from as early as 12 hours to as late as 13 days after infection.

Detection of the Virus

  • These tissue homogenates were tested for the presence of infectious EHV-1 using susceptible cell monolayers.
  • The research team employed indirect immunoperoxidase histochemistry on paraffin sections to monitor the expression of viral antigens.

Results

  • The results showed traces of both infectious EHV-1 and viral antigens present in lymph nodes associated with the respiratory tract as early as 12 hours post-exposure.
  • Infected leukocytes (white blood cells) were morphologically identified as lymphocytes, monocytes, macrophages, and plasma cells.

Implications

  • The rapid intracellular localization of EHV-1 in lymph nodes, as revealed in the study, implies the importance of cell-mediated immunity in the equine response to this virus.
  • This could guide further research and advancements in the treatment and prevention of EHV-1 infections, possibly by targeting cell-mediated immunity.

Cite This Article

APA
Kydd JH, Smith KC, Hannant D, Livesay GJ, Mumford JA. (1994). Distribution of equid herpesvirus-1 (EHV-1) in respiratory tract associated lymphoid tissue: implications for cellular immunity. Equine Vet J, 26(6), 470-473. https://doi.org/10.1111/j.2042-3306.1994.tb04052.x

Publication

Equine veterinary journal
ISSN: 0425-1644
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 26
Issue: 6
Pages: 470-473

Researcher Affiliations

Kydd, J H
  • Department of Infectious Diseases, Animal Health Trust, Newmarket, UK.
Smith, K C
    Hannant, D
      Livesay, G J
        Mumford, J A

          MeSH Terms

          • Animals
          • Antigens, Viral / analysis
          • Female
          • Herpesviridae Infections / immunology
          • Herpesviridae Infections / veterinary
          • Herpesvirus 1, Equid / immunology
          • Herpesvirus 1, Equid / isolation & purification
          • Horse Diseases / immunology
          • Horse Diseases / virology
          • Horses
          • Immunity, Cellular
          • Lymphoid Tissue / virology
          • Male
          • Respiratory Tract Infections / immunology
          • Respiratory Tract Infections / veterinary
          • Respiratory Tract Infections / virology
          • Virion / isolation & purification

          Grant Funding

          • Wellcome Trust

          Citations

          This article has been cited 26 times.
          1. Thieulent CJ, Sutton G, Toquet MP, Fremaux S, Hue E, Fortier C, Pléau A, Deslis A, Abrioux S, Guitton E, Pronost S, Paillot R. Oral Administration of Valganciclovir Reduces Clinical Signs, Virus Shedding and Cell-Associated Viremia in Ponies Experimentally Infected with the Equid Herpesvirus-1 C(2254) Variant.. Pathogens 2022 May 4;11(5).
            doi: 10.3390/pathogens11050539pubmed: 35631060google scholar: lookup
          2. Van Crombrugge E, Vanbeylen E, Van Cleemput J, Van den Broeck W, Laval K, Nauwynck H. Bacterial Toxins from Staphylococcus aureus and Bordetella bronchiseptica Predispose the Horse's Respiratory Tract to Equine Herpesvirus Type 1 Infection.. Viruses 2022 Jan 14;14(1).
            doi: 10.3390/v14010149pubmed: 35062352google scholar: lookup
          3. Zarski LM, Vaala WE, Barnett DC, Bain FT, Soboll Hussey G. A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1.. Front Vet Sci 2021;8:674850.
            doi: 10.3389/fvets.2021.674850pubmed: 34179166google scholar: lookup
          4. Laval K, Poelaert KCK, Van Cleemput J, Zhao J, Vandekerckhove AP, Gryspeerdt AC, Garré B, van der Meulen K, Baghi HB, Dubale HN, Zarak I, Van Crombrugge E, Nauwynck HJ. The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1.. Front Microbiol 2021;12:662686.
            doi: 10.3389/fmicb.2021.662686pubmed: 33746936google scholar: lookup
          5. Giessler KS, Samoilowa S, Soboll Hussey G, Kiupel M, Matiasek K, Sledge DG, Liesche F, Schlegel J, Fux R, Goehring LS. Viral Load and Cell Tropism During Early Latent Equid Herpesvirus 1 Infection Differ Over Time in Lymphoid and Neural Tissue Samples From Experimentally Infected Horses.. Front Vet Sci 2020;7:621.
            doi: 10.3389/fvets.2020.00621pubmed: 33102556google scholar: lookup
          6. Sutton G, Thieulent C, Fortier C, Hue ES, Marcillaud-Pitel C, Pléau A, Deslis A, Guitton E, Paillot R, Pronost S. Identification of a New Equid Herpesvirus 1 DNA Polymerase (ORF30) Genotype with the Isolation of a C(2254)/H(752) Strain in French Horses Showing no Major Impact on the Strain Behaviour.. Viruses 2020 Oct 13;12(10).
            doi: 10.3390/v12101160pubmed: 33066315google scholar: lookup
          7. Pavulraj S, Kamel M, Stephanowitz H, Liu F, Plendl J, Osterrieder N, Azab W. Equine Herpesvirus Type 1 Modulates Cytokine and Chemokine Profiles of Mononuclear Cells for Efficient Dissemination to Target Organs.. Viruses 2020 Sep 8;12(9).
            doi: 10.3390/v12090999pubmed: 32911663google scholar: lookup
          8. Oladunni FS, Horohov DW, Chambers TM. EHV-1: A Constant Threat to the Horse Industry.. Front Microbiol 2019;10:2668.
            doi: 10.3389/fmicb.2019.02668pubmed: 31849857google scholar: lookup
          9. Schnabel CL, Babasyan S, Rollins A, Freer H, Wimer CL, Perkins GA, Raza F, Osterrieder N, Wagner B. An Equine Herpesvirus Type 1 (EHV-1) Ab4 Open Reading Frame 2 Deletion Mutant Provides Immunity and Protection from EHV-1 Infection and Disease.. J Virol 2019 Nov 15;93(22).
            doi: 10.1128/JVI.01011-19pubmed: 31462575google scholar: lookup
          10. Brown LJ, Brown G, Kydd J, Stout TAE, Schulman ML. Failure to detect equid herpesvirus types 1 and 4 DNA in placentae and healthy new-born Thoroughbred foals.. J S Afr Vet Assoc 2019 May 30;90(0):e1-e5.
            doi: 10.4102/jsava.v90i0.1736pubmed: 31170779google scholar: lookup
          11. Poelaert KCK, Van Cleemput J, Laval K, Favoreel HW, Couck L, Van den Broeck W, Azab W, Nauwynck HJ. Equine Herpesvirus 1 Bridles T Lymphocytes To Reach Its Target Organs.. J Virol 2019 Apr 1;93(7).
            doi: 10.1128/JVI.02098-18pubmed: 30651370google scholar: lookup
          12. Garvey M, Lyons R, Hector RD, Walsh C, Arkins S, Cullinane A. Molecular Characterisation of Equine Herpesvirus 1 Isolates from Cases of Abortion, Respiratory and Neurological Disease in Ireland between 1990 and 2017.. Pathogens 2019 Jan 15;8(1).
            doi: 10.3390/pathogens8010007pubmed: 30650561google scholar: lookup
          13. Wimer CL, Schnabel CL, Perkins G, Babasyan S, Freer H, Stout AE, Rollins A, Osterrieder N, Goodman LB, Glaser A, Wagner B. The deletion of the ORF1 and ORF71 genes reduces virulence of the neuropathogenic EHV-1 strain Ab4 without compromising host immunity in horses.. PLoS One 2018;13(11):e0206679.
            doi: 10.1371/journal.pone.0206679pubmed: 30440016google scholar: lookup
          14. Poelaert KCK, Van Cleemput J, Laval K, Favoreel HW, Soboll Hussey G, Maes RK, Nauwynck HJ. Abortigenic but Not Neurotropic Equine Herpes Virus 1 Modulates the Interferon Antiviral Defense.. Front Cell Infect Microbiol 2018;8:312.
            doi: 10.3389/fcimb.2018.00312pubmed: 30258819google scholar: lookup
          15. Schnabel CL, Wimer CL, Perkins G, Babasyan S, Freer H, Watts C, Rollins A, Osterrieder N, Wagner B. Deletion of the ORF2 gene of the neuropathogenic equine herpesvirus type 1 strain Ab4 reduces virulence while maintaining strong immunogenicity.. BMC Vet Res 2018 Aug 22;14(1):245.
            doi: 10.1186/s12917-018-1563-4pubmed: 30134896google scholar: lookup
          16. Tallmadge RL, Žygelytė E, Van de Walle GR, Kristie TM, Felippe MJB. Effect of a Histone Demethylase Inhibitor on Equine Herpesvirus-1 Activity In Vitro.. Front Vet Sci 2018;5:34.
            doi: 10.3389/fvets.2018.00034pubmed: 29594155google scholar: lookup
          17. Shakya AK, O'Callaghan DJ, Kim SK. Comparative Genomic Sequencing and Pathogenic Properties of Equine Herpesvirus 1 KyA and RacL11.. Front Vet Sci 2017;4:211.
            doi: 10.3389/fvets.2017.00211pubmed: 29312962google scholar: lookup
          18. Wagner B, Perkins G, Babasyan S, Freer H, Keggan A, Goodman LB, Glaser A, Torsteinsdóttir S, Svansson V, Björnsdóttir S. Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age.. PLoS One 2017;12(1):e0169072.
            doi: 10.1371/journal.pone.0169072pubmed: 28045974google scholar: lookup
          19. Negussie H, Li Y, Tessema TS, Nauwynck HJ. Replication characteristics of equine herpesvirus 1 and equine herpesvirus 3: comparative analysis using ex vivo tissue cultures.. Vet Res 2016 Jan 15;47:19.
            doi: 10.1186/s13567-016-0305-5pubmed: 26768993google scholar: lookup
          20. Yeo WM, Osterrieder N, Stokol T. Equine herpesvirus type 1 infection induces procoagulant activity in equine monocytes.. Vet Res 2013 Mar 11;44(1):16.
            doi: 10.1186/1297-9716-44-16pubmed: 23497076google scholar: lookup
          21. Goodman LB, Wimer C, Dubovi EJ, Gold C, Wagner B. Immunological correlates of vaccination and infection for equine herpesvirus 1.. Clin Vaccine Immunol 2012 Feb;19(2):235-41.
            doi: 10.1128/CVI.05522-11pubmed: 22205656google scholar: lookup
          22. Azab W, Osterrieder N. Glycoproteins D of equine herpesvirus type 1 (EHV-1) and EHV-4 determine cellular tropism independently of integrins.. J Virol 2012 Feb;86(4):2031-44.
            doi: 10.1128/JVI.06555-11pubmed: 22171258google scholar: lookup
          23. Goodman LB, Loregian A, Perkins GA, Nugent J, Buckles EL, Mercorelli B, Kydd JH, Palù G, Smith KC, Osterrieder N, Davis-Poynter N. A point mutation in a herpesvirus polymerase determines neuropathogenicity.. PLoS Pathog 2007 Nov;3(11):e160.
            doi: 10.1371/journal.ppat.0030160pubmed: 17997600google scholar: lookup
          24. Molinková D, Celer V Jr, Jahn P. Isolation and partial characterization of equine herpesvirus type 1 in Czechia.. Folia Microbiol (Praha) 2004;49(5):605-11.
            doi: 10.1007/BF02931542pubmed: 15702554google scholar: lookup
          25. Bartels T, Steinbach F, Hahn G, Ludwig H, Borchers K. In situ study on the pathogenesis and immune reaction of equine herpesvirus type 1 (EHV-1) infections in mice.. Immunology 1998 Mar;93(3):329-34.
            doi: 10.1046/j.1365-2567.1998.00451.xpubmed: 9640242google scholar: lookup
          26. Sutton GA, Viel L, Carman PS, Boag BL. Pathogenesis and clinical signs of equine herpesvirus-1 in experimentally infected ponies in vivo.. Can J Vet Res 1998 Jan;62(1):49-55.
            pubmed: 9442940
          Subscribe to our newsletter
          Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.