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Developmental and comparative immunology2014; 46(2); 171-179; doi: 10.1016/j.dci.2014.04.001

Diversity of immunoglobulin lambda light chain gene usage over developmental stages in the horse.

Abstract: To further studies of neonatal immune responses to pathogens and vaccination, we investigated the dynamics of B lymphocyte development and immunoglobulin (Ig) gene diversity. Previously we demonstrated that equine fetal Ig VDJ sequences exhibit combinatorial and junctional diversity levels comparable to those of adult Ig VDJ sequences. Herein, RACE clones from fetal, neonatal, foal, and adult lymphoid tissue were assessed for Ig lambda light chain combinatorial, junctional, and sequence diversity. Remarkably, more lambda variable genes (IGLV) were used during fetal life than later stages and IGLV gene usage differed significantly with time, in contrast to the Ig heavy chain. Junctional diversity measured by CDR3L length was constant over time. Comparison of Ig lambda transcripts to germline revealed significant increases in nucleotide diversity over time, even during fetal life. These results suggest that the Ig lambda light chain provides an additional dimension of diversity to the equine Ig repertoire.
Copyright © 2014 Elsevier Ltd. All rights reserved.
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Publication Date: 2014-04-12 PubMed ID: 24726757PubMed Central: PMC4107094DOI: 10.1016/j.dci.2014.04.001Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper investigates the dynamics of B lymphocyte development and immunoglobulin gene diversity in horses during different stages of development. It uncovers that lambda variable genes (IGLV) usage and nucleotide diversity increase over time, suggesting that the Ig lambda light chain provides significant diversity to the equine Ig repertoire.

B lymphocyte development and Immunoglobulin gene diversity

  • The researchers have attempted to understand the dynamics of B lymphocyte development and immunoglobulin (Ig) gene diversity over different developmental stages of a horse.
  • Prior to this study, it had been established that equine fetal Ig VDJ sequences exhibit combinatorial and junctional diversity levels comparable to adult Ig VDJ sequences. This study extends this previous knowledge.
  • For this study, they used clones from fetal, neonatal, foal, and adult lymphoid tissue.

Ig lambda light chain combinatorial, junctional, and sequence diversity

  • The researchers have focused on assessing lambda light chain combinatorial, junctional, and sequence diversity during different developmental stages.
  • The study revealed that more lambda variable genes (IGLV) were used during fetal life than later stages like in neonatal, foal, or adult stage. The usage of IGLV genes significantly varies with time. This observation was in contrast to Ig heavy chain, where no such variability was observed.
  • The junctional diversity measured by CDR3L length remained constant over time, irrespective of the development phase.

Increase in nucleotide diversity over time

  • The study reported a significant increase in nucleotide diversity over time. This trend was seen even during the fetal life stage.
  • The researchers compared Ig lambda transcripts to germline and found considerable increases in nucleotide diversity as the development progressed.
  • Based on these findings, the authors proposed that the Ig lambda light chain contributes to an additional dimension of diversity to the equine Ig repertoire.

Cite This Article

APA
Tallmadge RL, Tseng CT, Felippe MJ. (2014). Diversity of immunoglobulin lambda light chain gene usage over developmental stages in the horse. Dev Comp Immunol, 46(2), 171-179. https://doi.org/10.1016/j.dci.2014.04.001

Publication

Developmental and comparative immunology
ISSN: 1879-0089
NlmUniqueID: 7708205
Country: United States
Language: English
Volume: 46
Issue: 2
Pages: 171-179
PII: S0145-305X(14)00097-4

Researcher Affiliations

Tallmadge, Rebecca L
  • Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States. Electronic address: rlt8@cornell.edu.
Tseng, Chia T
  • Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States.
Felippe, M Julia B
  • Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States.

MeSH Terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / physiology
  • Fetus / metabolism
  • Gene Expression Regulation, Developmental
  • Horses / genetics
  • Horses / metabolism
  • Immunoglobulin lambda-Chains / genetics
  • Immunoglobulin lambda-Chains / metabolism
  • Molecular Sequence Annotation
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • V(D)J Recombination

Grant Funding

  • DP2 OD007216 / NIH HHS
  • DP2OD007216 / NIH HHS

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Citations

This article has been cited 8 times.
  1. Wibmer CK, Mashilo P. Exploiting V-Gene Bias for Rapid, High-Throughput Monoclonal Antibody Isolation from Horses. Viruses 2022 Sep 30;14(10).
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  2. Tallmadge RL, Wang M, Sun Q, Felippe MJB. Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses. PLoS One 2018;13(9):e0202646.
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  4. Tallmadge RL, Miller SC, Parry SA, Felippe MJB. Antigen-specific immunoglobulin variable region sequencing measures humoral immune response to vaccination in the equine neonate. PLoS One 2017;12(5):e0177831.
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