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Zentralblatt fur Veterinarmedizin. Reihe A1996; 43(9); 573-576; doi: 10.1111/j.1439-0442.1996.tb00489.x

Immunohistochemical examination of light-chain expression (lambda/kappa ratio) in canine, feline, equine, bovine and porcine plasma cells.

Abstract: Five species of domestic animals were examined immunohistochemically and the light-chain ratios of the immunoglobulins produced by plasma cells analysed. Forty dogs, 11 cats, 10 horses, 11 cattle and 14 pigs were tested using the sequential indirect immunoperoxidase and immunophosphatase methods. Tissues from the tonsils, spleen and cervical lymph nodes were analysed. It could be seen that the lambda/kappa ratio in dogs, cats, horses and cattle is largely dominated by the lambda chains (lambda/kappa ratio in dogs: 91/9%, in cats; 92/8%; in horses: 96/4%; in cattle: 91/9%). A more or less balanced ratio was observed only in the case of the pigs (52/48%).
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Publication Date: 1996-11-01 PubMed ID: 8968166DOI: 10.1111/j.1439-0442.1996.tb00489.xGoogle Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper investigates the light-chain ratios of the immunoglobulins produced by plasma cells in five species of domestic animals including dogs, cats, horses, cattle, and pigs. These investigations are executed through immunohistochemical examination.

Research Objective and Methods

  • The study aims to explore the light-chain expression in the immunoglobulins produced by plasma cells of various domestic animals. The specific goal is to determine the lambda/kappa ratio of this expression for different species.
  • The method used for this investigation is immunohistochemical examination. This is a technique that involves using antibodies to detect specific proteins in cells or tissue sections, thus allowing researchers to visually examine and quantify the presence of these proteins.
  • The animals that were included in the study are forty dogs, eleven cats, ten horses, eleven cattle, and fourteen pigs.
  • The researchers applied sequential indirect immunoperoxidase and immunophosphatase methods. These are both staining techniques, employed to determine the presence and distribution of the proteins of interest.
  • The tissues areas analysed were collected from the tonsils, spleen, and cervical lymph nodes of the animals.

Findings and Conclusion

  • In dogs, cats, horses, and cattle, the lambda/kappa ratio was dominated largely by the lambda chains. The lambda/kappa ratio in dogs was 91/9%, in cats; 92/8%; in horses: 96/4%; and in cattle: 91/9%.
  • Conversely, a more balanced ratio was noted in pigs, revealing a lambda/kappa ratio of 52/48%.
  • This indicates a species-difference in the light-chain expression in the subject animals. That is, in dogs, cats, horses, and cattle, the production of lambda chains tended to dominate, whereas in pigs, the production ratio was almost equal.
  • The findings are beneficial in providing insights into the immunological aspects of different species. It highlights potential implications on how each species responds to pathogens and their inherent disease resistance. However, further investigations would be needed to directly assess these aspects.
  • Moreover, the researchers might conduct a follow-up study to explore the reasons behind such variations in light-chain ratio across different species and whether these differences might have effects on these species’ overall health.

Cite This Article

APA
Arun SS, Breuer W, Hermanns W. (1996). Immunohistochemical examination of light-chain expression (lambda/kappa ratio) in canine, feline, equine, bovine and porcine plasma cells. Zentralbl Veterinarmed A, 43(9), 573-576. https://doi.org/10.1111/j.1439-0442.1996.tb00489.x

Publication

Zentralblatt fur Veterinarmedizin. Reihe A
ISSN: 0514-7158
NlmUniqueID: 0331323
Country: Germany
Language: English
Volume: 43
Issue: 9
Pages: 573-576

Researcher Affiliations

Arun, S S
  • Institute of Veterinary Pathology, University of Munich, Germany.
Breuer, W
    Hermanns, W

      MeSH Terms

      • Animals
      • Cats
      • Cattle
      • Dogs
      • Horses / blood
      • Horses / immunology
      • Immunoglobulin Light Chains / analysis
      • Immunoglobulin Light Chains / blood
      • Immunoglobulin kappa-Chains / analysis
      • Immunoglobulin kappa-Chains / blood
      • Immunoglobulin lambda-Chains / analysis
      • Immunoglobulin lambda-Chains / blood
      • Immunohistochemistry
      • Lymph Nodes / immunology
      • Palatine Tonsil / immunology
      • Plasma Cells / immunology
      • Spleen / immunology
      • Swine / blood
      • Swine / immunology

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