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bioRxiv : the preprint server for biology2023; 2023.05.04.539352; doi: 10.1101/2023.05.04.539352

Diversity of the bacterial and viral communities in the tropical horse tick, Dermacentor nitens in Colombia.

Abstract: Ticks are obligatory hematophagous ectoparasites that transmit pathogens among various vertebrates, including humans. The composition of the microbial and viral communities in addition to the pathogenic microorganisms is highly diverse in ticks, but the factors driving the diversity are not well understood. The tropical horse tick, , is distributed throughout the Americas and it is recognized as a natural vector of and , the causal agents of equine piroplasmosis. We characterized the bacterial and viral communities associated with partially-fed females collected by a passive survey on horses from field sites representing three distinct geographical areas in Colombia (Bolivar, Antioquia, and Cordoba). RNA-seq and sequencing of the V3 and V4 hypervariable regions of the 16S rRNA gene were performed using the Illumina-Miseq platform. A total of 356 operational taxonomic units (OTUs) were identified, in which the presumed endosymbiotic Francisellaceae/ spp. was predominantly found. Nine contigs corresponding to six different viruses were identified in three viral families: Chuviridae, Rhabdoviridae, and Flaviviridae. Differences in the relative abundance of the microbial composition among the geographical regions were found to be independent of the presence of -Like Endosymbiont (FLE). The most prevalent bacteria found on each region were in Bolivar, in Antioquia, and in Cordoba. -like endosymbionts, mainly recognized as the etiological agent of rickettsioses in Colombia were detected in the Cordoba samples. Metatranscriptomics revealed 13 contigs containing FLE genes, suggesting a trend of regional differences. These findings suggest regional distinctions among the ticks and their bacterial compositions.
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Publication Date: 2023-05-05 PubMed ID: 37205465PubMed Central: PMC10187316DOI: 10.1101/2023.05.04.539352Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper investigates the bacterial and viral diversity in the tropical horse tick found in distinct geographical regions in Colombia, aiming to understand regional variations and factors driving this diversity. The study identified a total of 356 bacterial units and nine viral contigs belonging to three different viral families.

Tick Collection and Sequencing

  • The researchers collected partially-fed female ticks from horses in three distinct regions in Colombia: Bolivar, Antioquia, and Cordoba. These ticks were then subjected to RNA sequencing and 16S rRNA gene sequencing using the Illumina-Miseq platform.

Identification of Bacteria and Viruses

  • A total of 356 operational taxonomic units (OTUs) were identified, with the predominant bacteria being of the endosymbiotic Francisellaceae/Coxiella spp.
  • Six different viruses were also found, grouped into three viral families: Chuviridae, Rhabdoviridae, and Flaviviridae.

Geographical Differences in Microbial Composition

  • The study found differences in the relative abundance of the microbial composition among the three geographic regions.
  • The most prevalent bacteria in each region were Anaplasma in Bolivar, Coxiella in Antioquia, and Ehrlichia in Cordoba.
  • Interestingly, these differences were found to be independent of the presence of Francisella-Like Endosymbiont (FLE), another pervasive bacterium.

Presence of Rickettsioses Etiological Agent

  • Rickettsia, a type of bacteria recognized as the causative agent of rickettsiosis (a group of serious bacterial infections), was detected in the samples from Cordoba.

Identified Gene Contigs

  • Metatranscriptomics, the study of RNA molecules, revealed 13 contigs (parts of the virus or bacterium) containing FLE genes, suggesting regional differences.

Conclusion

  • The results suggest the existence of regional distinctions among the ticks and their associated bacterial compositions, which may have implications for the transmission of various diseases by these ticks across different regions.

Cite This Article

APA
Holguin-Rocha AF, Calle-Tobon A, Vásquez GM, Astete H, Fisher ML, Tobon-Castano A, Velez-Tobon G, Maldonado-Ruiz LP, Silver K, Park Y, Londono-Renteria B. (2023). Diversity of the bacterial and viral communities in the tropical horse tick, Dermacentor nitens in Colombia. bioRxiv, 2023.05.04.539352. https://doi.org/10.1101/2023.05.04.539352

Publication

bioRxiv : the preprint server for biology
NlmUniqueID: 101680187
Country: United States
Language: English
PII: 2023.05.04.539352

Researcher Affiliations

Holguin-Rocha, Andres F
  • Department of Entomology, Kansas State University, Manhattan, KS, USA.
Calle-Tobon, Arley
  • Grupo Entomologia Medica, Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia.
Vásquez, Gissella M
  • U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Bellavista, Callao, Peru.
Astete, Helvio
  • U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Bellavista, Callao, Peru.
Fisher, Michael L
  • U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Bellavista, Callao, Peru.
  • Navy Warfare Development Center, Norfolk, VA (Current Affiliation).
Tobon-Castano, Alberto
  • Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia.
Velez-Tobon, Gabriel
  • Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia.
Maldonado-Ruiz, L Paulina
  • Department of Entomology, Kansas State University, Manhattan, KS, USA.
Silver, Kristopher
  • Department of Entomology, Kansas State University, Manhattan, KS, USA.
Park, Yoonseong
  • Department of Entomology, Kansas State University, Manhattan, KS, USA.
Londono-Renteria, Berlin
  • School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, USA.

Grant Funding

  • R21 AI163423 / NIAID NIH HHS

Conflict of Interest Statement

Conflict of Interest. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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