DNA fingerprinting in horses using a simple (TG)n probe and its application to population comparisons.
Abstract: A synthetic polynucleotide (TG)n was hybridized to equine DNA digested with HinfI and hypervariable hybridization patterns were obtained. Mendelian inheritance of these DNA fingerprinting patterns was confirmed by pedigree analysis. Estimates of the probabilities of identical band patterns in unrelated individuals of different breeds (Swedish Trotters, North Swedish Trotters, Thoroughbreds and Arabians) were in the range 1 x 10(-4) - 7 x 10(-6). The variability derived with the (TG)n probe in horses was higher than what we obtained with several other commonly used probes for DNA fingerprinting. Individuals within breeds tended to be more similar to each other with regard to DNA fingerprint pattern than to individuals of other breeds. Moreover, a parsimony analysis made on the basis of the hybridization patterns gave clustering of individuals within breeds. The possibility of using hypervariable probes for the identification of breed-specific characters is discussed.
Publication Date: 1992-01-01 PubMed ID: 1570891
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the use of a basic DNA sequencing probe for the unique genetic identification of horses and explores its potential use for breed comparison and identification.
Methodology and Results
- The scientists synthesized a basic DNA sequence described as (TG)n, which they hybridized to horse DNA that had been broken down with a substance called HinfI. The term “hybridization” in this context refers to the process of combining two complementary strands of DNA. This process generated what are termed “hypervariable” hybridization patterns, or distinct, easily distinguished DNA fingerprints.
- Analyzing the lineage of selected horses confirmed that these DNA fingerprints followed the laws of Mendelian inheritance, meaning they were passed on from parent to offspring in predictable patterns based on established genetic rules.
- They estimated the likelihood of an exact match in the DNA fingerprinting patterns amongst non-related horses from different breeds to be between 1 in 10,000 to 1 in 1,000,000. The breeds studied included Swedish Trotters, North Swedish Trotters, Thoroughbreds, and Arabians.
- The study found that the (TG)n probe demonstrated a higher degree of variability compared to typically used DNA fingerprinting probes, providing a more detailed view of genetic diversity amongst equines.
Comparison and Application
- Regarding breed comparison, the research found that DNA fingerprints of horses within the same breed bore closer resemblance to each other than to horses from other breeds. This demonstrates the potential use of such probes for breed identification and comparison.
- Additionally, a parsimony analysis (a statistical method that infers phylogenies under a particular model of evolution) based on these fingerprints showed consistent clustering within breeds.
- With these results, the team discussed the potential for using these highly variable probes in determining breed-specific characteristics and genetic traits, which would have applications in areas like breeding, conservation, and disease susceptibility studies.
Cite This Article
APA
Ellegren H, Andersson L, Johansson M, Sandberg K.
(1992).
DNA fingerprinting in horses using a simple (TG)n probe and its application to population comparisons.
Anim Genet, 23(1), 1-9.
Publication
Researcher Affiliations
- Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
MeSH Terms
- Animals
- Biological Evolution
- Breeding
- DNA Fingerprinting
- DNA Probes
- Genetics, Population
- Guanine Nucleotides
- Horses / genetics
- Nucleic Acid Hybridization
- Polymorphism, Genetic
- Polynucleotides
- Thymine Nucleotides
Citations
This article has been cited 5 times.- Ramakishana W, Lagu MD, Gupta VS, Ranjekar PK. DNA fingerprinting in rice using oligonucleotide probes specific for simple repetitive DNA sequences. Theor Appl Genet 1994 Jun;88(3-4):402-6.
- Rothuizen J, Wolfswinkel J, Lenstra JA, Frants RR. The incidence of mini- and micro-satellite repetitive DNA in the canine genome. Theor Appl Genet 1994 Oct;89(4):403-6.
- Ramakrishna W, Chowdari KV, Lagu MD, Gupta VS, Ranjekar PK. DNA fingerprinting to detect genetic variation in rice using hypervariable DNA sequences. Theor Appl Genet 1995 Jun;90(7-8):1000-6.
- Zajc I, Mellersh CS, Sampson J. Variability of canine microsatellites within and between different dog breeds. Mamm Genome 1997 Mar;8(3):182-5.
- Ellegren H, Hartman G, Johansson M, Andersson L. Major histocompatibility complex monomorphism and low levels of DNA fingerprinting variability in a reintroduced and rapidly expanding population of beavers. Proc Natl Acad Sci U S A 1993 Sep 1;90(17):8150-3.
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