DNA methylation patterns of the S100A14, POU2F3 and SFN genes in equine sarcoid tissues.
Abstract: Genetic and epigenetic alterations in the equine sarcoid, a locally invasive skin tumour of equids, are still poorly characterized. Numerous studies have provided reliable evidence for the relationship between the development of cancer and the loss of function of a number of tumour suppressor genes. In the present study, we assessed methylation levels in the promoter region of SFN, S100A14 and POU2F3 genes in sarcoid samples to clarify whether DNA methylation may be associated with previously identified changes in the expression level of these genes during the course of tumour progression. Using bisulfite sequencing and clone sequencing, we detected that lesional samples had a significantly higher rate of DNA methylation in the analyzed S100A14A region than the corresponding normal skin tissue. A frequent methylation of the SFN and POU2F3 promoter sequences were observed in both the tumour samples and the control skin tissues. Further studies are needed to evaluate the role of aberrant methylation in sarcoid progression and to understand the mechanisms involved in reduced expression of SFN, S100A14 and POU2F3 genes in the lesional tissues.
Copyright © 2018 Elsevier Ltd. All rights reserved.
Publication Date: 2018-07-24 PubMed ID: 30086514DOI: 10.1016/j.rvsc.2018.07.006Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research investigates the potential relationships between altered DNA methylation levels of certain genes (S100A14, POU2F3, SFN) and the progression of equine sarcoid, a type of invasive skin tumor in equines. It was evident that these DNA modifications could be associated with changes in gene expression during tumor growth.
Study Context
- The focus of this study is on equine sarcoid, which is a locally invasive skin tumor that affects equines.
- Understanding the genetic and epigenetic changes in this disease can help uncover the mechanisms behind its progression.
- Previous studies have indicated a link between the development of cancers and the alteration in function of tumour suppressor genes.
Research Methods and Findings
- The researchers examined the levels of methylation in the promoter regions of three specific genes – SFN, S100A14, and POU2F3 – in sarcoid samples.
- The purpose of assessing these methylation levels was to establish whether DNA methylation had any correlation with the changes in expression levels of these genes during tumor progression.
- The team used advanced techniques like bisulfite sequencing and clone sequencing to carry out the assessment.
- The study revealed that lesional samples (those taken from diseased tissue) showed a significantly higher rate of DNA methylation in the S100A14A region when compared to normal skin tissue.
- They also noted frequent methylation in the SFN and POU2F3 promoter regions in both the tumor and control skin tissues.
Implications and Future Research
- The study’s findings indicate a potential relationship between the level of DNA methylation in certain regions of the genome and the progression of tumor growth in equine sarcoids.
- However, further research is required to better understand the role these methylation aberrations play in tumor growth, and the mechanisms that lead to reduced gene expression in the affected tissues.
Cite This Article
APA
Semik-Gurgul E, Ząbek T, Fornal A, Wnuk M, Pawlina-Tyszko K, Gurgul A, Klukowska-Rötzler J, Koch C, Mählmann K, Bugno-Poniewierska M.
(2018).
DNA methylation patterns of the S100A14, POU2F3 and SFN genes in equine sarcoid tissues.
Res Vet Sci, 119, 302-307.
https://doi.org/10.1016/j.rvsc.2018.07.006 Publication
Researcher Affiliations
- National Research Institute of Animal Production, Department of Animal Genomics and Molecular Biology, Krakowska 1, 32-083 Balice, Poland. Electronic address: ewelina.semik@izoo.krakow.pl.
- National Research Institute of Animal Production, Department of Animal Genomics and Molecular Biology, Krakowska 1, 32-083 Balice, Poland. Electronic address: t.zabek@izoo.krakow.pl.
- National Research Institute of Animal Production, Department of Animal Genomics and Molecular Biology, Krakowska 1, 32-083 Balice, Poland. Electronic address: agnieszka.fornal@izoo.krakow.pl.
- Department of Genetics, Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland.
- National Research Institute of Animal Production, Department of Animal Genomics and Molecular Biology, Krakowska 1, 32-083 Balice, Poland. Electronic address: klaudia.pawlina@izoo.krakow.pl.
- National Research Institute of Animal Production, Department of Animal Genomics and Molecular Biology, Krakowska 1, 32-083 Balice, Poland. Electronic address: artur.gurgul@izoo.krakow.pl.
- Swiss Institute of Equine Medicine ISME, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern and Agroscope, Länggassstrasse 124c, Postfach 8466, CH-3001 Bern, Switzerland; Department of Emergency Medicine, University Hospital Bern, Inselspital, 3010 Bern, Switzerland.
- Swiss Institute of Equine Medicine ISME, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern and Agroscope, Länggassstrasse 124c, Postfach 8466, CH-3001 Bern, Switzerland. Electronic address: christoph.koch@vetsuisse.unibe.ch.
- Swiss Institute of Equine Medicine ISME, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern and Agroscope, Länggassstrasse 124c, Postfach 8466, CH-3001 Bern, Switzerland; Equine Clinic, General Surgery and Radiology, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany. Electronic address: Kathrin.Maehlmann@fu-berlin.de.
- National Research Institute of Animal Production, Department of Animal Genomics and Molecular Biology, Krakowska 1, 32-083 Balice, Poland; Institute of Veterinary Sciences, University of Agriculture in Krakow, al., Mickiewicza 24/28, 30-059 Kraków, Poland. Electronic address: monika.bugno@izoo.krakow.pl.
MeSH Terms
- Animals
- DNA Methylation
- Epigenomics
- Gene Expression Regulation, Neoplastic / physiology
- Horse Diseases / metabolism
- Horses
- Promoter Regions, Genetic
- Skin
- Skin Neoplasms
Citations
This article has been cited 2 times.- Semik-Gurgul E, Gurgul A, Szmatoła T. Transcriptome and methylome sequencing reveals altered long non-coding RNA genes expression and their aberrant DNA methylation in equine sarcoids.. Funct Integr Genomics 2023 Aug 8;23(3):268.
- Pawlina-Tyszko K, Semik-Gurgul E, Ząbek T, Witkowski M. Methylation Status of Gene Bodies of Selected microRNA Genes Associated with Neoplastic Transformation in Equine Sarcoids.. Cells 2022 Jun 14;11(12).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
