Doping control analysis of antipsychotics and other prohibited substances in equine plasma by liquid chromatography/tandem mass spectrometry.

This research outlines the development of an efficient method using liquid chromatography/tandem mass spectrometry (LC/MS/MS) for identifying over 80 banned substances, including antipsychotics, in horse plasma. The substances can be detected at extremely low concentrations, highlighting the method’s accuracy and potential for use in equestrian sports regulation.

Significance of the Study

• The research addresses a critical issue in equestrian sports, where substances like antipsychotics that have no valid use are often misused or abused. This practice goes against the International Agreement for Breeding, Racing and Wagering by the International Federation of Horseracing Authorities, as well as the policies of the Fédération Equestrian Internationale (FEI).
• There’s a lack of comprehensive detection strategies for these substances in equine samples within existing literature, which this study helps to address.

The Methodology

• The researchers have developed a method using liquid chromatography/tandem mass spectrometry (LC/MS/MS), a highly efficient detection procedure.
• The procedure begins with solid-phase extraction (SPE), a sample preparation process isolating the desired substances from the samples.
• It allows the simultaneous detection of more than 80 prohibited substances, proving its versatility.
• The method is potent enough to detect these substances at extraordinarily low concentrations ranging from sub-parts-per-billion (ppb) to low-ppb levels in equine plasma, demonstrating its precise detection capability.

Potential Impact

• This new method could serve as a robust tool for authorities to enforce doping regulations in equestrian sports and horseracing.
• Its efficiency and sensitivity could lead to a significant reduction in substance misuse, promoting fair competition and the overall health and welfare of horses in these sports.
• By detecting substances at very low concentrations, it could deter potential substance abuse by making it difficult for wrongdoers to avoid detection.