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Home / Equine Research Bank / Mol Hum Reprod / Dynamics of extracellular vesicle-coupled microRNAs in equin...
Molecular human reproduction2023; 29(4); doi: 10.1093/molehr/gaad009

Dynamics of extracellular vesicle-coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation.

Abstract: Innumerable similarities in reproductive cyclicity and hormonal alterations highlight the considerable utility of the mare to study aspects of follicular dynamics and reproductive function in view of the largely constricted, human research subjects. The bi-directional communication between the growing oocyte and the surrounding somatic cells embodies the hallmark of mammalian follicular development, partially mediated by extracellular vesicles (EVs) encapsulated with microRNAs (miRNAs) and present in the follicular fluid (FF). Here, we aimed to decipher the dynamics of the miRNAs in EVs from equine FF aspirated in vivo during different stages of follicular development, namely, predeviation (PreDev; 18-20 mm), deviation (Dev; 22-25 mm), postdeviation (PostDev; 26-29 mm), preovulatory (PreOV; 30-35 mm), and impending ovulation (IMP; ∼40 mm). Approximately 176 known miRNAs were found in all groups with 144 mutually detected among all groups. Cluster analysis exhibited 15 different expression patterns during follicular development. Among these patterns, a group of 22 miRNAs (including miR-146b-5p, miR-140, and miR-143) exhibited a sharp reduction in expression from the PreDev until the PreOV stage. Another cluster of 23 miRNAs (including miR-106b, miR-199a-5p, and miR-125a-5p) exhibited a stable expression pattern at the PreDev stage until the PostDev stage, with a significant increase at the PreOV stage followed by a significant decrease at the IMP stage. In conclusion, this study provides greater insights into the stage-specific expression dynamics of FF EV-miRNAs during equine follicular development, which may propose novel approaches to improve ART and provide new biomarkers to facilitate the assessment of ovarian pathophysiological conditions.
© The Author(s) 2023. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please email: journals.permissions@oup.com.
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Publication Date: 2023-03-01 PubMed ID: 36852862PubMed Central: PMC10321592DOI: 10.1093/molehr/gaad009Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • N.I.H.
  • Extramural

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article studies the changes in the micro-RNAs found in the fluid of horse ovaries during different stages of follicular development. This may provide new ways to improve assisted reproductive techniques and help diagnose ovarian diseases.

Background

  • The study’s objective is to understand the role and changes in microRNAs present in the extracellular vesicles of follicular fluid during the different stages of follicular development.
  • Follicular development in mammals is marked by communication between the growing egg and the surrounding cells, partially facilitated by extracellular vesicles and their encapsulated microRNAs in the follicular fluid.
  • This research was conducted on mares due to their significant similarities with human reproductive cyclicity and hormonal changes.

Method

  • The fluid from equine ovaries was extracted at different stages of follicular development:
    • Predeviation (18-20mm)
    • Deviation (22-25mm)
    • Postdeviation (26-29mm)
    • Preovulatory (30-35mm)
    • Impending ovulation (approximately 40mm)
  • Around 176 known microRNAs were identified in all groups, with 144 of these being found in each group.

Findings

  • The study revealed 15 different patterns of microRNA expression during the follicular development stages.
  • Sudden reduction in the expression of a group of 22 microRNAs (including miR-146b-5p, miR-140, and miR-143) was noted from the pre-deviation until the pre-ovulatory stage.
  • Another cluster of 23 microRNAs (including miR-106b, miR-199a-5p, and miR-125a-5p) showed stable expression from the PreDev stage until the PostDev stage, followed by a significant increase at the PreOV stage, then a sharp decrease at the impending ovulation stage.

Conclusion

  • The research offers novel insights into the dynamics of microRNAs’ expression in follicular fluid at different stages of equine follicular development.
  • This knowledge could lead to the development of new strategies for improving Assisted Reproductive Technologies(ART) and provide new biomarkers to facilitate better diagnosis and understanding of ovarian pathophysiological conditions.

Cite This Article

APA
Gebremedhn S, Gad A, Ishak GM, Menjivar NG, Gastal MO, Feugang JM, Prochazka R, Tesfaye D, Gastal EL. (2023). Dynamics of extracellular vesicle-coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation. Mol Hum Reprod, 29(4). https://doi.org/10.1093/molehr/gaad009

Publication

Molecular human reproduction
ISSN: 1460-2407
NlmUniqueID: 9513710
Country: England
Language: English
Volume: 29
Issue: 4

Researcher Affiliations

Gebremedhn, Samuel
  • Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
  • Division of IVF Research, Genus Plc, DeForest, WI, USA.
Gad, Ahmed
  • Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
  • Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt.
Ishak, Ghassan M
  • Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
  • Department of Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, USA.
Menjivar, Nico G
  • Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
Gastal, Melba O
  • Department of Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, USA.
Feugang, Jean M
  • Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, USA.
Prochazka, Radek
  • Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic.
Tesfaye, Dawit
  • Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
Gastal, Eduardo L
  • Department of Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, USA.

MeSH Terms

  • Horses
  • Animals
  • Humans
  • Female
  • Follicular Fluid / metabolism
  • MicroRNAs / metabolism
  • Ovarian Follicle / metabolism
  • Ovulation / genetics
  • Extracellular Vesicles / genetics
  • Extracellular Vesicles / metabolism
  • Mammals

Grant Funding

  • Ministry of Higher Education & Scientific Research, Baghdad

Conflict of Interest Statement

There is no conflict of interest to declare.

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