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Involvement of miRNAs in equine follicle development.
Abstract: Previous evidence from in vitro studies suggests specific roles for a subset of miRNAs, including miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212, in regulating ovarian follicle development. The objective of this study was to determine changes in the levels of these miRNAs in relation to follicle selection, maturation, and ovulation in the monovular equine ovary. In Experiment 1, follicular fluid was aspirated during ovulatory cycles from the dominant (DO) and largest subordinate (S) follicles of an ovulatory wave and the dominant (DA) follicle of a mid-cycle anovulatory wave (n=6 mares). Follicular fluid levels of progesterone and estradiol were lower (P<0.01) in S follicles than in DO follicles, whereas mean levels of IGF1 were lower (P<0.01) in S and DA follicles than in DO follicles. Relative to DO and DA follicles, S follicles had higher (P≤0.01) follicular fluid levels of miR-145 and miR-378. In Experiment 2, follicular fluid and granulosa cells were aspirated from dominant follicles before (DO) and 24 h after (L) administration of an ovulatory dose of hCG (n=5 mares/group). Relative to DO follicles, L follicles had higher follicular fluid levels of progesterone (P=0.05) and lower granulosa cell levels of CYP19A1 and LHCGR (P<0.005). Levels of miR-21, miR-132, miR-212, and miR-224 were increased (P<0.05) in L follicles; this was associated with reduced expression of the putative miRNA targets, PTEN, RASA1, and SMAD4. These novel results may indicate a physiological involvement of miR-21, miR-145, miR-224, miR-378, miR-132, and miR-212 in the regulation of cell survival, steroidogenesis, and differentiation during follicle selection and ovulation in the monovular ovary.
Publication Date: 2013-07-31 PubMed ID: 23813447DOI: 10.1530/REP-13-0107Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research explores the role of certain miRNAs in the development and maturation of ovarian follicles in horses. The findings reveal that these miRNAs may contribute to cell survival, steroid production, and cell differentiation during follicle selection and ovulation.
Objective and Methodology of the Study
- The study aimed to understand the changes in levels of a specific group of miRNAs (miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212) during the selection, maturation, and ovulation of ovarian follicles in horses.
- The analysis involved two experiments. In Experiment 1, follicular fluid was collected from different types of follicles during ovulatory cycles in horses. In Experiment 2, follicular fluid and granulosa cells were collected from dominant follicles before and after the administration of a hormone that triggers ovulation.
Key Findings from Experiment 1
- Follicles identified as subordinate (S) displayed lower levels of progesterone, estradiol, and insulin-like growth factor 1 (IGF1) in comparison to dominant ovulatory (DO) follicles.
- Subordinate follicles exhibited higher levels of miR-145 and miR-378.
Key Findings from Experiment 2
- Follicles observed after the administration of an ovulation-inducing hormone (L follicles) showed higher levels of progesterone, but lower levels of two key proteins involved in hormone synthesis and response.
- L follicles also displayed increased levels of miR-21, miR-132, miR-212, and miR-224. The rise in these miRNAs was coupled with a reduction of their potential target proteins, affecting cell survival and differentiation.
Implications of the Study
- The changes in levels of specific miRNAs in different stages of follicle development suggest their important role in the ovulatory process.
- The identified miRNAs regulate cell survival, steroid production, and cell differentiation during follicle selection and ovulation.
- The findings offer a deeper understanding of the molecular mechanisms underlying ovulation, which could potentially contribute to improving reproductive management strategies in horses and potentially in other species.
Cite This Article
APA
Schauer SN, Sontakke SD, Watson ED, Esteves CL, Donadeu FX.
(2013).
Involvement of miRNAs in equine follicle development.
Reproduction, 146(3), 273-282.
https://doi.org/10.1530/REP-13-0107 Publication
Researcher Affiliations
- The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh, Midlothian EH25 9RG, UK.
MeSH Terms
- Animals
- Chorionic Gonadotropin / physiology
- Dinoprostone / metabolism
- Female
- Follicular Fluid / physiology
- Horses / physiology
- Insulin-Like Growth Factor I / physiology
- MicroRNAs / physiology
- Ovarian Follicle / metabolism
- Random Allocation
- Steroids / physiology
Grant Funding
- Biotechnology and Biological Sciences Research Council
Citations
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