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Home / Equine Research Bank / Emerg Infect Dis / Eastern equine encephalomyelitis virus infection in a horse ...
Emerging infectious diseases2002; 8(3); 283-288; doi: 10.3201/eid0803.010199

Eastern equine encephalomyelitis virus infection in a horse from California.

Abstract: A yearling quarter horse, which was raised in southern California, received routine vaccinations for prevention of infection by Eastern equine encephalomyelitis virus (EEEV). One week later, severe neurologic signs developed, and the horse was humanely destroyed. A vaccine-related encephalomyelitis was later suspected. A final diagnosis of EEEV infection was established on the basis of acute onset of the neurologic signs, histopathologic and serologic testing, and isolation and molecular characterization of EEEV from brain tissue. The vaccine was extensively tested for viral inactivation. Nucleotide sequences from the vaccine and the virus isolated in the affected horse were also compared. In California, arboviral encephalomyelitides are rarely reported, and EEEV infection has not previously been documented. This report describes the occurrence of EEEV infection in the horse and the investigation to determine the source of infection, which was not definitively identified.
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Publication Date: 2002-04-03 PubMed ID: 11927026PubMed Central: PMC2732474DOI: 10.3201/eid0803.010199Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study reports a case of Eastern equine encephalomyelitis virus (EEEV) infection in a horse from California. Despite being vaccinated, the horse developed severe neurological symptoms and the cause was traced back to a EEEV infection.

Background

  • The subject of the study was a year-old quarter horse raised in southern California.
  • As part of its regular healthcare, the horse received vaccinations to prevent EEEV infection.
  • Within a week of receiving the vaccination, the horse developed severe neurological symptoms.
  • Due to its deteriorating health condition, the horse was euthanized.

Diagnosis and Testing

  • Initial suspicions pointed to vaccine-related encephalomyelitis – a negative reaction to the vaccine causing inflammation of the spinal cord and brain.
  • The final diagnosis was an EEEV infection, based on the acute onset of neurological symptoms, histopathologic and serologic tests, and the isolation and molecular characterization of EEEV from brain tissue.
  • Nucleotide sequence comparison was performed between the vaccine sample and the virus isolated from the horse to verify the source of the infection.
  • The researchers also extensively tested the vaccine for viral inactivation to ensure that it was not the source of the infection.

An Unexpected Finding

  • Cases of arboviral encephalomyelitides, including EEEV infections, are seldom reported in California.
  • In fact, this is the first documented case of an EEEV infection in the state.
  • However, the exact source of the horse’s infection could not be definitively determined from the investigation.

Importance of the Study

  • The study confirms the presence of EEEV in California, expanding the known range of the virus.
  • It emphasizes the need for improved surveillance and reporting of arboviral infections.
  • It underscores the importance of thorough vaccine testing and monitoring for potential vaccine-related complications.

Cite This Article

APA
Franklin RP, Kinde H, Jay MT, Kramer LD, Green EG, Chiles RE, Ostlund E, Husted S, Smith J, Parker MD. (2002). Eastern equine encephalomyelitis virus infection in a horse from California. Emerg Infect Dis, 8(3), 283-288. https://doi.org/10.3201/eid0803.010199

Publication

Emerging infectious diseases
ISSN: 1080-6040
NlmUniqueID: 9508155
Country: United States
Language: English
Volume: 8
Issue: 3
Pages: 283-288

Researcher Affiliations

Franklin, Robert P
  • Humphrey, Giacopuzzi & Associates Equine Hospital, Somis, CA, USA. franklinr@mail.vetmed.ufl.edu
Kinde, Hailu
    Jay, Michele T
      Kramer, Laura D
        Green, Emily-Gene N
          Chiles, Robert E
            Ostlund, Eileen
              Husted, Stan
                Smith, Jonathan
                  Parker, Michael D

                    MeSH Terms

                    • Animals
                    • California
                    • Encephalitis Virus, Eastern Equine / genetics
                    • Encephalomyelitis, Acute Disseminated / pathology
                    • Encephalomyelitis, Acute Disseminated / veterinary
                    • Encephalomyelitis, Eastern Equine / pathology
                    • Encephalomyelitis, Eastern Equine / veterinary
                    • Horse Diseases / virology
                    • Horses
                    • Male
                    • RNA, Viral
                    • Reverse Transcriptase Polymerase Chain Reaction

                    References

                    This article includes 22 references
                    1. Summers BA, Cummings JF, deLahunta A. Veterinary neuropathology. 1st ed. St. Louis (MO): Mosby Year Book Inc; 1995.
                    2. Whitley RJ. Viral encephalitis.. N Engl J Med 1990 Jul 26;323(4):242-50.
                      pubmed: 2195341doi: 10.1056/nejm199007263230406google scholar: lookup
                    3. McLean RG, Frier G, Parham GL, Francy DB, Monath TP, Campos EG, Therrien A, Kerschner J, Calisher CH. Investigations of the vertebrate hosts of eastern equine encephalitis during an epizootic in Michigan, 1980.. Am J Trop Med Hyg 1985 Nov;34(6):1190-202.
                      pubmed: 3010751doi: 10.4269/ajtmh.1985.34.1190google scholar: lookup
                    4. Fenner FJ, Gibbs EP, Murphy FA, Rott R, Studdert MJ, White DO. Veterinary virology. 2nd ed. San Diego (CA): Academic Press; 1993.
                    5. Bertone JJ. Togaviral Encephalitis. In: Reed SM, Bayly WM, editors. Equine internal medicine. Philadelphia: Saunders, 1998;502-3.
                    6. Calisher CH. Medically important arboviruses of the United States and Canada.. Clin Microbiol Rev 1994 Jan;7(1):89-116.
                      pmc: PMC358307pubmed: 8118792doi: 10.1128/cmr.7.1.89google scholar: lookup
                    7. Reisen WK, Kramer LD, Chiles RE, Green EG, Martinez VM. Encephalitis virus persistence in California birds: preliminary studies with house finches.. J Med Entomol 2001 May;38(3):393-9.
                      pubmed: 11372964doi: 10.1603/0022-2585-38.3.393google scholar: lookup
                    8. Kramer LD, Fallah HM. Genetic variation among isolates of western equine encephalomyelitis virus from California.. Am J Trop Med Hyg 1999 Apr;60(4):708-13.
                      pubmed: 10348252doi: 10.4269/ajtmh.1999.60.708google scholar: lookup
                    9. Brault AC, Powers AM, Chavez CL, Lopez RN, Cachón MF, Gutierrez LF, Kang W, Tesh RB, Shope RE, Weaver SC. Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America.. Am J Trop Med Hyg 1999 Oct;61(4):579-86.
                      pubmed: 10548292doi: 10.4269/ajtmh.1999.61.579google scholar: lookup
                    10. Hui LT, Husted SR, Reisen WK, Myers CM, Ascher MS, Kramer VL. Summary of reported St. Louis encephalitis and western equine encephalomyelitis virus activity in California from 1969-1997. Proceedings and Papers of the 67th Annual Conference of the Mosquito and Vector Control Association of California 1999. Vol. 67. Elk Gorve (CA): Mosuito and Vector Control Association of California. p. 61-72.
                    11. Reeves WC. Epidemiology and control of mosquito-borne arboviruses in California, 1983-1987. Sacramento (CA): California Mosquito Vector Control Association, Inc.; 1990.
                    12. CHAMBERLAIN RW, SUDIA WD. The North American arthropod-borne encephalitis viruses in Culex tarsalis Coquillett.. Am J Hyg 1957 Sep;66(2):151-9.
                      pubmed: 13458180doi: 10.1093/oxfordjournals.aje.a119892google scholar: lookup
                    13. Grady GF, Maxfield HK, Hildreth SW, Timperi RJ Jr, Gilfillan RF, Rosenau BJ, Francy DB, Calisher CH, Marcus LC, Madoff MA. Eastern equine encephalitis in Massachusetts, 1957-1976. A prospective study centered upon analyses of mosquitoes.. Am J Epidemiol 1978 Feb;107(2):170-8.
                      pubmed: 23676doi: 10.1093/oxfordjournals.aje.a112519google scholar: lookup
                    14. McLintock J. The arbovirus problem in Canada.. Can J Public Health 1976 May-Jun;67 Suppl 1:8-12.
                      pubmed: 963664
                    15. Sellers RF. Weather, host and vector--their interplay in the spread of insect-borne animal virus diseases.. J Hyg (Lond) 1980 Aug;85(1):65-102.
                      pmc: PMC2134001pubmed: 6131919doi: 10.1017/s0022172400027108google scholar: lookup
                    16. Bohart RM, Washino RK. Mosquitoes of California. 3rd ed. Berkeley:University of California, Division of Agricultural Sciences; 1978.
                    17. Brown F. An overview of the inactivation of FMDV and the implications when residual virus is present in vaccines.. Dev Biol Stand 1991;75:37-41.
                      pubmed: 1665462
                    18. Brown F. Review of accidents caused by incomplete inactivation of viruses.. Dev Biol Stand 1993;81:103-7.
                      pubmed: 8174792
                    19. Melnick JL. Virus inactivation: lessons from the past.. Dev Biol Stand 1991;75:29-36.
                      pubmed: 1794629
                    20. Nathanson N, Langmuir AD. The Cutter incident. Poliomyelitis following formaldehyde-inactivated poliovirus vaccination in the United States during the Spring of 1955. II. Relationship of poliomyelitis to Cutter vaccine. 1963.. Am J Epidemiol 1995 Jul 15;142(2):109-40; discussion 107-8.
                      pubmed: 7598112doi: 10.1093/oxfordjournals.aje.a117611google scholar: lookup
                    21. Kinney RM, Tsuchiya KR, Sneider JM, Trent DW. Molecular evidence for the origin of the widespread Venezuelan equine encephalitis epizootic of 1969 to 1972.. J Gen Virol 1992 Dec;73 ( Pt 12):3301-5.
                      doi: 10.1099/0022-1317-73-12-3301pubmed: 1469368google scholar: lookup
                    22. Weaver SC, Powers AM, Brault AC, Barrett AD. Molecular epidemiological studies of veterinary arboviral encephalitides.. Vet J 1999 Mar;157(2):123-38.
                      doi: 10.1053/tvjl.1998.0289pubmed: 10204408google scholar: lookup

                    Citations

                    This article has been cited 13 times.
                    1. Gil LHVG, Magalhaes T, Santos BSAS, Oliveira LV, Oliveira-Filho EF, Cunha JLR, Fraiha ALS, Rocha BMM, Longo BC, Ecco R, Faria GC, Furtini R, Drumond SRM, Maranhão RPA, Lobato ZIP, Guedes MIMC, Teixeira RBC, Costa EA. Active Circulation of Madariaga Virus, a Member of the Eastern Equine Encephalitis Virus Complex, in Northeast Brazil.. Pathogens 2021 Aug 3;10(8).
                      doi: 10.3390/pathogens10080983pubmed: 34451447google scholar: lookup
                    2. Câmara RJF, Bueno BL, Resende CF, Balasuriya UBR, Sakamoto SM, Reis JKPD. Viral Diseases that Affect Donkeys and Mules.. Animals (Basel) 2020 Nov 25;10(12).
                      doi: 10.3390/ani10122203pubmed: 33255568google scholar: lookup
                    3. Azar SR, Campos RK, Bergren NA, Camargos VN, Rossi SL. Epidemic Alphaviruses: Ecology, Emergence and Outbreaks.. Microorganisms 2020 Aug 1;8(8).
                      doi: 10.3390/microorganisms8081167pubmed: 32752150google scholar: lookup
                    4. Baxter VK, Heise MT. Immunopathogenesis of alphaviruses.. Adv Virus Res 2020;107:315-382.
                      doi: 10.1016/bs.aivir.2020.06.002pubmed: 32711733google scholar: lookup
                    5. Kumar B, Manuja A, Gulati BR, Virmani N, Tripathi BN. Zoonotic Viral Diseases of Equines and Their Impact on Human and Animal Health.. Open Virol J 2018;12:80-98.
                      doi: 10.2174/1874357901812010080pubmed: 30288197google scholar: lookup
                    6. Erasmus JH, Rossi SL, Weaver SC. Development of Vaccines for Chikungunya Fever.. J Infect Dis 2016 Dec 15;214(suppl 5):S488-S496.
                      doi: 10.1093/infdis/jiw271pubmed: 27920179google scholar: lookup
                    7. Pedersen K, Marks DR, Wang E, Eastwood G, Weaver SC, Goldstein SM, Sinnett DR, DeLiberto TJ. Widespread Detection of Antibodies to Eastern Equine Encephalitis, West Nile, St. Louis Encephalitis, and Turlock Viruses in Various Species of Wild Birds from Across the United States.. Am J Trop Med Hyg 2016 Jul 6;95(1):206-11.
                      doi: 10.4269/ajtmh.15-0840pubmed: 27162269google scholar: lookup
                    8. Goodman CH, Russell BJ, Velez JO, Laven JJ, Bagarozzi DA Jr, Moon JL, Bedi K, Johnson BW. Production of a Sindbis/Eastern Equine Encephalitis chimeric virus inactivated cell culture antigen.. J Virol Methods 2015 Oct;223:19-24.
                      doi: 10.1016/j.jviromet.2015.07.007pubmed: 26205552google scholar: lookup
                    9. Weise WJ, Hermance ME, Forrester N, Adams AP, Langsjoen R, Gorchakov R, Wang E, Alcorn MD, Tsetsarkin K, Weaver SC. A novel live-attenuated vaccine candidate for mayaro Fever.. PLoS Negl Trop Dis 2014 Aug;8(8):e2969.
                      doi: 10.1371/journal.pntd.0002969pubmed: 25101995google scholar: lookup
                    10. Pandya J, Gorchakov R, Wang E, Leal G, Weaver SC. A vaccine candidate for eastern equine encephalitis virus based on IRES-mediated attenuation.. Vaccine 2012 Feb 8;30(7):1276-82.
                      doi: 10.1016/j.vaccine.2011.12.121pubmed: 22222869google scholar: lookup
                    11. Arrigo NC, Watts DM, Frolov I, Weaver SC. Experimental infection of Aedes sollicitans and Aedes taeniorhynchus with two chimeric Sindbis/Eastern equine encephalitis virus vaccine candidates.. Am J Trop Med Hyg 2008 Jan;78(1):93-7.
                      pubmed: 18187790
                    12. Wang E, Petrakova O, Adams AP, Aguilar PV, Kang W, Paessler S, Volk SM, Frolov I, Weaver SC. Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice.. Vaccine 2007 Oct 23;25(43):7573-81.
                      doi: 10.1016/j.vaccine.2007.07.061pubmed: 17904699google scholar: lookup
                    13. Burnouf T, Griffiths E, Padilla A, Seddik S, Stephano MA, Gutiérrez JM. Assessment of the viral safety of antivenoms fractionated from equine plasma.. Biologicals 2004 Sep;32(3):115-28.
                      doi: 10.1016/j.biologicals.2004.07.001pubmed: 15536042google scholar: lookup
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