Ectopic transplantation of equine invasive trophoblast.
Abstract: A system for transplanting invasive equine trophoblast (i.e., chorionic girdle) to ectopic sites has been developed as a means to study the differentiation of this tissue and to assess maternal immune responses to the conceptus tissue in a site outside the uterus. Chorionic girdle was isolated from Day 33 to 34 conceptuses and surgically placed into the vulvar mucosa or subdermal skin of recipient mares. Biopsy specimens of the graft sites for immunohistochemical staining were taken at weekly or biweekly intervals after grafting. Serum samples were collected from each recipient and tested for antibody to donor major histocompatibility complex (MHC) class I antigens using the lymphocyte microcytotoxicity assay. Transplanted trophoblast cells expressed differentiation markers associated with invading chorionic girdle and endometrial cup cells. The transplanted trophoblast cells were also labeled by an antibody to eCG. Strong cellular and humoral immune responses to the transplanted tissue were mounted by the recipients, similar to those occurring during normal equine pregnancy. Despite these responses, the invasive trophoblast transplants survived for at least 28 days after grafting and downregulated MHC class I antigens, as do the mature endometrial cup cells in equine pregnancy. These findings suggest that invasive equine trophoblast has the capacity to differentiate fully in equine nonuterine tissues, and that it can evade maternal immune responses independent of the physiological state of pregnancy and in sites other than the uterus.
Publication Date: 2001-02-24 PubMed ID: 11207188DOI: 10.1095/biolreprod64.3.753Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This research paper pertains to the study about transplantation of invasive equine trophoblast, or chorionic girdle, into different parts of a mare’s body to observe tissue differentiation and the resulting maternal immune responses outside of the uterus.
Methodology
- The chorionic girdle was obtained from equine conceptuses at Day 33 to 34. These were then surgically placed into the vulvar mucosa or subdermal skin of recipient mares.
- Graft site biopsy specimens were collected either weekly or biweekly for immunohistochemical staining. Meanwhile, serum samples from recipients were obtained and tested for antibody reactivity towards donor major histocompatibility complex (MHC) class I antigens. The testing was done by utilizing the lymphocyte microcytotoxicity assay.
Results
- Findings revealed that the transplanted trophoblast cells expressed differentiation markers akin to those seen in invading chorionic girdle and endometrial cup cells. The transplanted cells were also labelled by an antibody to equine Chorionic Gonadotropin (eCG).
- The recipient mares exhibited robust cellular and humoral immune responses to the transplanted tissue. These immune responses were found to be similar to the ones that occur during a normal equine pregnancy.
- The trophoblast transplants survived for at least 28 days post grafting, despite the strong immune responses. They even downregulated MHC Class I antigens, much like mature endometrial cup cells observed in equine pregnancies.
Conclusions
- The study concludes that the invasive equine trophoblast can fully differentiate in equine nonuterine tissues. Additionally, it is found capable of evading maternal immune responses outside the normal physiological state of pregnancy and in areas beyond the uterus.
These findings provide significant understanding in the field of equine reproductive biology and potentially open new avenues for further research and clinical applications.
Cite This Article
APA
Adams AP, Antczak DF.
(2001).
Ectopic transplantation of equine invasive trophoblast.
Biol Reprod, 64(3), 753-763.
https://doi.org/10.1095/biolreprod64.3.753 Publication
Researcher Affiliations
- Equine Genetics Center, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA. apa7@cornell.edu
MeSH Terms
- Animals
- Antibodies, Monoclonal
- Biopsy / veterinary
- CD4-CD8 Ratio / veterinary
- Cell Differentiation / physiology
- Cell Transplantation
- Chorion / cytology
- Chorion / immunology
- Cytotoxicity Tests, Immunologic / veterinary
- Female
- Genes, MHC Class I / immunology
- Histocompatibility Antigens Class I / biosynthesis
- Histocompatibility Antigens Class I / immunology
- Horses / embryology
- Horses / immunology
- Immunohistochemistry / veterinary
- Insemination, Artificial / veterinary
- Male
- Pregnancy
- Skin / immunology
- Trophoblasts / cytology
- Trophoblasts / immunology
- Trophoblasts / transplantation
- Uterus / immunology
- Vulva / immunology
Grant Funding
- F32 HD08575 / NICHD NIH HHS
- NICHD-15799 / NICHD NIH HHS
- NICHD-34086 / NICHD NIH HHS
- T32 RR07059 / NCRR NIH HHS
Citations
This article has been cited 16 times.- Antczak DF, Allen WRT. Placentation in Equids.. Adv Anat Embryol Cell Biol 2021;234:91-128.
- Lee JK, Oh SJ, Park H, Shin OS. Recent Updates on Research Models and Tools to Study Virus-Host Interactions at the Placenta.. Viruses 2019 Dec 18;12(1).
- Nergadze SG, Piras FM, Gamba R, Corbo M, Cerutti F, McCarter JGW, Cappelletti E, Gozzo F, Harman RM, Antczak DF, Miller D, Scharfe M, Pavesi G, Raimondi E, Sullivan KF, Giulotto E. Birth, evolution, and transmission of satellite-free mammalian centromeric domains.. Genome Res 2018 Jun;28(6):789-799.
- Berglund AK, Schnabel LV. Allogeneic major histocompatibility complex-mismatched equine bone marrow-derived mesenchymal stem cells are targeted for death by cytotoxic anti-major histocompatibility complex antibodies.. Equine Vet J 2017 Jul;49(4):539-544.
- Brosnahan MM, Silvela EJ, Crumb J, Miller DC, Erb HN, Antczak DF. Ectopic Trophoblast Allografts in the Horse Resist Destruction by Secondary Immune Responses.. Biol Reprod 2016 Dec;95(6):135.
- Pezzanite LM, Fortier LA, Antczak DF, Cassano JM, Brosnahan MM, Miller D, Schnabel LV. Equine allogeneic bone marrow-derived mesenchymal stromal cells elicit antibody responses in vivo.. Stem Cell Res Ther 2015 Apr 12;6(1):54.
- Antczak DF, de Mestre AM, Wilsher S, Allen WR. The equine endometrial cup reaction: a fetomaternal signal of significance.. Annu Rev Anim Biosci 2013 Jan;1:419-42.
- Harman RM, Bussche L, Ledbetter EC, Van de Walle GR. Establishment and characterization of an air-liquid canine corneal organ culture model to study acute herpes keratitis.. J Virol 2014 Dec;88(23):13669-77.
- Rapacz-Leonard A, Dąbrowska M, Janowski T. Major histocompatibility complex I mediates immunological tolerance of the trophoblast during pregnancy and may mediate rejection during parturition.. Mediators Inflamm 2014;2014:579279.
- Brosnahan MM, Miller DC, Adams M, Antczak DF. IL-22 is expressed by the invasive trophoblast of the equine (Equus caballus) chorionic girdle.. J Immunol 2012 May 1;188(9):4181-7.
- Noronha LE, Huggler KE, de Mestre AM, Miller DC, Antczak DF. Molecular evidence for natural killer-like cells in equine endometrial cups.. Placenta 2012 May;33(5):379-86.
- de Mestre AM, Hanlon D, Adams AP, Runcan E, Leadbeater JC, Erb HN, Costa CC, Miller D, Allen WR, Antczak DF. Functions of ectopically transplanted invasive horse trophoblast.. Reproduction 2011 Jun;141(6):849-56.
- de Mestre A, Noronha L, Wagner B, Antczak DF. Split immunological tolerance to trophoblast.. Int J Dev Biol 2010;54(2-3):445-55.
- de Mestre AM, Miller D, Roberson MS, Liford J, Chizmar LC, McLaughlin KE, Antczak DF. Glial cells missing homologue 1 is induced in differentiating equine chorionic girdle trophoblast cells.. Biol Reprod 2009 Feb;80(2):227-34.
- Adams AP, Oriol JG, Campbell RE, Oppenheim YC, Allen WR, Antczak DF. The effect of skin allografting on the equine endometrial cup reaction.. Theriogenology 2007 Jul 15;68(2):237-47.
- Golos TG. Pregnancy initiation in the rhesus macaque: towards functional manipulation of the maternal-fetal interface.. Reprod Biol Endocrinol 2004 Jun 16;2:35.
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