Effect of a competitive inhibitor of platelet aggregation on experimentally induced laminitis in ponies.
Abstract: To determining whether inhibition of platelet aggregation prevents development of carbohydrate overload-induced alimentary laminitis. Methods: 22 healthy adult ponies. Methods: Acute laminitis was induced by oral administration of corn starch/wood flour to 16 ponies, 8 of which were treated with a synthetic analogue of the platelet fibrinogen receptor antagonist peptide (RPR) RGDS (arginine-glycine-aspartic acid-serine) 110885; 6 ponies served as negative controls. Blood was collected before and at 4, 8, 12, 24, 28, and 32 hours after administration of carbohydrate overload, and PCV, total plasma protein concentration, platelet count, activated clotting time, whole blood recalcification time, spontaneous platelet aggregation, ex vivo platelet aggregation responses, in vivo platelet activation, and platelet-neutrophil aggregates were evaluated. Results: Of 16 ponies given carbohydrate, 6 of 8 untreated ponies developed laminitis and 0 of 8 ponies treated with RPR 110885 developed laminitis. The RPR 110885 treatment attenuated the increase in platelet-neutrophil aggregates observed in untreated ponies. Conclusions: Platelets are involved in the pathogenesis of equine alimentary laminitis. Conclusions: Platelet aggregation inhibitors may be useful for prevention or treatment of laminitis, or both.
Publication Date: 1998-07-11 PubMed ID: 9659543
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates whether the use of a synthetic analogue of platelet fibrinogen receptor antagonist peptide could prevent laminitis in ponies induced by carbohydrate overload. The study results indicate that inhibiting platelet aggregation might be a viable option for the prevention or treatment of laminitis.
Research Methodology
- The research conducted involved 22 healthy adult ponies.
- Acute laminitis, a disease condition in horses causing hoof pain and lameness, was induced in 16 of these ponies through the oral administration of a mixture of corn starch and wood flour to overload their carbohydrate intake.
- Out of the 16 ponies, 8 were treated with a synthetic analogue of the platelet fibrinogen receptor antagonist peptide, RPR 110885.
- The remaining 8 ponies were not treated with the synthetic analogue and acted as a control group for the experiment.
- Another group of 6 ponies were used as negative controls; they were not given the carbohydrate overload or any treatment.
- Blood was collected from all the ponies at various points in time before and after the administration of the carbohydrate overload to evaluate several factors including platelet count, plasma protein concentration, clotting time, and platelet-neutrophil aggregates.
Findings of the Study
- Out of the 8 untreated ponies (control group) that were given the carbohydrate overload, 6 developed laminitis.
- None of the 8 ponies that were treated with the synthetic analogue RPR 110885 developed laminitis.
- The study found that the increase in platelet-neutrophil aggregates, that was observed in the untreated ponies, was significantly reduced in the ponies treated with RPR 110885.
Conclusions from the Research
- The research concludes that the platelets play a significant role in the development of equine laminitis.
- The synthetic analogue of the platelet fibrinogen receptor antagonist peptide (RPR 110885) was found to be effective in preventing the incidence of laminitis in ponies.
- Based on the outcomes of this research, the use of inhibitors of platelet aggregation could be a potential strategy for the treatment or prevention of laminitis in ponies.
Cite This Article
APA
Weiss DJ, Evanson OA, McClenahan D, Fagliari JJ, Dunnwiddie CT, Wells RE.
(1998).
Effect of a competitive inhibitor of platelet aggregation on experimentally induced laminitis in ponies.
Am J Vet Res, 59(7), 814-817.
Publication
Researcher Affiliations
- Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA.
MeSH Terms
- Animals
- Blood Platelets / drug effects
- Blood Platelets / physiology
- Female
- Foot Diseases / blood
- Foot Diseases / drug therapy
- Foot Diseases / veterinary
- Horse Diseases / blood
- Horse Diseases / drug therapy
- Horses
- Lameness, Animal / blood
- Lameness, Animal / chemically induced
- Lameness, Animal / drug therapy
- Male
- Neutrophils / drug effects
- Neutrophils / physiology
- Oligopeptides / therapeutic use
- Platelet Aggregation
- Platelet Aggregation Inhibitors / therapeutic use
- Platelet Count / drug effects
- Wood
- Zea mays
Citations
This article has been cited 2 times.- Martinez-Pereira MA, Franceschi Rda C, Coelho BP, Fünkler Gda R, Zancan DM. Experimental model of tympanic colic (acute abdomen) in chinchillas (Chinchilla lanigera).. Lab Anim Res 2014 Sep;30(3):136-41.
- Menzies-Gow NJ, Wray H, Bailey SR, Harris PA, Elliott J. The effect of tumour necrosis factor-α and insulin on equine digital blood vessel function in vitro.. Inflamm Res 2014 Aug;63(8):637-47.
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