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Translational animal science2024; 8; txae172; doi: 10.1093/tas/txae172

Effect of a dietary nutraceutical “STRUCTURE-Joint” on response of horses to intra-articular challenge with IL-1: implications for tissue adaptation to stress.

Abstract: The purpose was to determine local (articular) and systemic effects of intra-articular interleukin-1 in horses supplemented with a dietary PUFA supplement [STRUCTURE-Joint (ST-J)]. Sixteen (16) healthy, mature, light breed horses were randomly assigned to diets containing 0 or 120 mL (n = 8 per group) of ST-J for 30 d. On days 0 (prior to beginning supplementation) and 27, recombinant equine interleukin-1β (reIL-1 β) (75 ng) was injected into the left or right intercarpal joint to induce mild, transient synovitis. Synovial fluid was obtained by aseptic arthrocentesis at postinjection hour 0 (immediately prior to IL-1 injection), 6, 12, and 72. ST-J supplementation for 30 d significantly increased synovial fluid nitric oxide, and resolvin D1 compared with the unsupplemented control group and significantly increased PGE2 levels and reduced joint circumference in the ST-J treated horses on day 30 compared to the same group of horses on day 0. There was also a significant increase in plasma hemoglobin, free and total bilirubin, and decrease in plasma glucose. These data provide evidence for the usefulness of ST-J to modulate physiological variables with importance in exercise performance and tissue adaptation to exercise stress and further research on this product is warranted.
© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.
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Publication Date: 2024-12-07 PubMed ID: 39713786PubMed Central: PMC11660166DOI: 10.1093/tas/txae172Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study tests the effects of a dietary supplement for horses, named “STRUCTURE-Joint”, on the response of horses to an immune system challenge induced by interleukin-1. The study found that the supplement appeared to modulate several physiological variables linked to stress response and performance during exercise.

Research Method

  • The research was carried out on 16 healthy, mature, light breed horses. The horses were randomly split into two groups, one of which was given 120mL of the STRUCTURE Joint supplement for a period of 30 days, while the other group was not supplemented.
  • Both before the supplementation began and on the 27th day of the experiment, an immune system challenge was artificially induced in the horses using interleukin-1, an important compound in inflammatory responses. The interleukin-1 was injected into the left or right intercarpal joint of the horses to cause a mild, temporary inflammation.
  • Synovial fluid was collected from the horses at several points after each interleukin-1 injection to track any changes over time – immediately after the injection and 6, 12, and 72 hours afterwards.

Findings

  • After 30 days, the horses that had been given the STRUCTURE-Joint supplement showed a significant increase in the levels of nitric oxide and resolvin D1 in their synovial fluid compared to the non-supplemented horses. These substances are associated with controlling inflammatory responses, dilating blood vessels, and reducing pain perception.
  • There was also a noticeable increase in PGE levels, which could have potential benefits in managing tissue response to inflammation and injury, and joint circumference was reduced in the supplemented horses on day 30 in comparison to measurements taken on day 0.
  • Other changes noted in the supplemented group included a significant increase in plasma hemoglobin and bilirubin – indicators of breakdown and recycling of red blood cells – and a decrease in plasma glucose.

Interpretation and Implications

  • Joint supplements for horses help maintain healthy joints, mobility, and connective tissue, supporting performance and comfort as horses age.
  • The changes observed in the horses that were given the STRUCTURE-Joint supplement suggest that the supplement might be capable of influencing various physiological processes that are important in exercise performance and the body’s adaptation to stress through exercise.
  • The authors of the research note that their findings suggest the dietary nutraceutical STRUCTURE-Joint has delivered beneficial outcomes under test conditions and warrant further study.

Cite This Article

APA
Korac L, Golestani N, MacNicol J, Souccar-Young J, Witherspoon S, Wildish A, Topfer S, Pearson W. (2024). Effect of a dietary nutraceutical “STRUCTURE-Joint” on response of horses to intra-articular challenge with IL-1: implications for tissue adaptation to stress. Transl Anim Sci, 8, txae172. https://doi.org/10.1093/tas/txae172

Publication

Translational animal science
ISSN: 2573-2102
NlmUniqueID: 101738705
Country: England
Language: English
Volume: 8
Pages: txae172

Researcher Affiliations

Korac, Lindsay
  • Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Golestani, Nadia
  • Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
MacNicol, Jennifer
  • Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Souccar-Young, Jamie
  • Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Witherspoon, Sophie
  • Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Wildish, Arayih
  • Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Topfer, Sydney
  • Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Pearson, Wendy
  • Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.

Conflict of Interest Statement

None declared.

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Citations

This article has been cited 1 times.
  1. Korac L, St George L, MacNicol J, McCrae P, Jung L, Golestani N, Karrow N, Cánovas A, Pearson W. Functional and biochemical inflammatory responses to low-dose intra-articular recombinant equine IL-1β: a pilot study. Front Vet Sci 2025;12:1746738.
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