Journal of animal physiology and animal nutrition2015; 100(3); 565-577; doi: 10.1111/jpn.12359

Influence of an n-3 long-chain polyunsaturated fatty acid-enriched diet on experimentally induced synovitis in horses.

Abstract: Dietary n-3 long-chain polyunsaturated fatty acid (LCPUFA) supplementation has previously been shown to modify joint-related inflammation in several species, although information in the horse is lacking. We investigated whether dietary supplementation with n-3 LCPUFA would modify experimentally induced synovitis in horses. Twelve, skeletally mature, non-pregnant mares were randomly assigned to either a control diet (CONT) or an n-3 long-chain fatty acid-enriched treatment diet (N3FA) containing 40 g/day of n-3 LCPUFA for 91 days. Blood samples taken on days 0, 30, 60 and 90, and synovial fluid collected on days 0 and 90 were processed for lipid composition. On day 91, joint inflammation was stimulated using an intra-articular (IA) injection of 100 ng of recombinant equine IL-1beta (reIL-1β). Synovial fluid samples taken at post-injection hours (PIH) 0, 4, 8 and 24 were analysed for prostaglandin E2 (PGE2 ), matrix metalloproteinase (MMP) activity and routine cytology. Synovium and articular cartilage samples collected at PIH 8 were analysed for gene expression of MMP 1 and MMP 13, interleukin-1beta (IL-1β), cyclooxygenase 2 (COX-2), tumour necrosis factor-alpha and the aggrecanases, a disintegrin and metalloprotease with thrombospondin motifs (ADAMTS)-4 and ADAMTS-5. A 90-day feeding period of n-3 LCPUFA increased serum phospholipid and synovial fluid lipid compositions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) compared to CONT horses. The reIL-1β injection caused an inflammatory response; however, there was no effect of dietary treatment on synovial fluid PGE2 content and MMP activity. Synovial tissue collected from N3FA horses exhibited lower expression of ADAMTS-4 compared to CONT horses. Despite the presence of EPA and DHA in the synovial fluid of N3FA horses, dietary n-3 LCPUFA supplementation did not modify synovial fluid biomarkers compared to CONT horses; however, the lower ADAMTS-4 mRNA expression in N3FA synovium warrants further investigation of n-3 LCPUFA as a joint therapy.
Publication Date: 2015-07-20 PubMed ID: 26189710DOI: 10.1111/jpn.12359Google Scholar: Lookup
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  • Journal Article

Summary

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This research explored whether a diet enriched with n-3 long-chain polyunsaturated fatty acid (LCPUFA) could affect joint inflammation in horses. The study did not find any significant changes in synovial fluid biomarkers, however, there was a decrease in ADAMTS-4 mRNA expression in horses fed this diet, suggesting more research is needed.

Methodology

  • Twelve mature, non-pregnant mares were randomly assigned to either a control diet (CONT) or an n-3 long-chain fatty acid-enriched treatment diet (N3FA) containing 40 g/day of n-3 LCPUFA for a period of 91 days.
  • The researchers collected blood samples on days 0, 30, 60 and 90, and synovial fluid on days 0 and 90 processed them for lipid composition.
  • On day 91, joint inflammation was induced using an intra-articular injection of recombinant equine IL-1beta (reIL-1β).
  • Synovial fluid samples were collected again at post-injection hours (PIH) 0, 4, 8 and 24, analyzed for prostaglandin E2 (PGE2), matrix metalloproteinase (MMP) activity and routine cytology.
  • The researchers also analyzed the synovium and articular cartilage samples for gene expressions of MMP 1, MMP 13, interleukin-1beta (IL-1β), COX-2, tumor necrosis factor-alpha, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4 and ADAMTS-5.

Results

  • It was observed that a 90-day feeding period of n-3 LCPUFA increased serum phospholipid and synovial fluid lipid compositions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) compared to the CONT horses.
  • The reIL-1β injection caused an inflammatory response but the dietary treatment’s effect on synovial fluid PGE2 content and MMP activity was inconclusive.
  • The synovial tissue collected from N3FA horses exhibited lower expression of ADAMTS-4 compared to those on a CONT diet.

Conclusion

  • The research found no significant changes in synovial fluid biomarkers between dietary treatments, indicating that dietary n-3 LCPUFA supplementation did not significantly modify joint inflammation in horses.
  • However, noticing the lower count of ADAMTS-4 mRNA expression in N3FA horses shows cause to further investigate the potential value of n-3 LCPUFA as joint therapy in horses.

Cite This Article

APA
Ross-Jones TN, McIlwraith CW, Kisiday JD, Hess TM, Hansen DK, Black J. (2015). Influence of an n-3 long-chain polyunsaturated fatty acid-enriched diet on experimentally induced synovitis in horses. J Anim Physiol Anim Nutr (Berl), 100(3), 565-577. https://doi.org/10.1111/jpn.12359

Publication

ISSN: 1439-0396
NlmUniqueID: 101126979
Country: Germany
Language: English
Volume: 100
Issue: 3
Pages: 565-577

Researcher Affiliations

Ross-Jones, T N
  • Department of Animal Science, Colorado State University, Fort Collins, CO, USA.
McIlwraith, C W
  • Gail Holmes Equine Orthopaedic Research Center, Department of Clinical Sciences, Colorado State University, Fort Collins, CO, USA.
Kisiday, J D
  • Gail Holmes Equine Orthopaedic Research Center, Department of Clinical Sciences, Colorado State University, Fort Collins, CO, USA.
Hess, T M
  • Department of Animal Science, Colorado State University, Fort Collins, CO, USA.
Hansen, D K
  • Department of Animal Science, Colorado State University, Fort Collins, CO, USA.
Black, J
  • Department of Animal Science, Colorado State University, Fort Collins, CO, USA.
  • Gail Holmes Equine Orthopaedic Research Center, Department of Clinical Sciences, Colorado State University, Fort Collins, CO, USA.

MeSH Terms

  • Animal Feed / analysis
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Diet / veterinary
  • Fatty Acids, Omega-3 / administration & dosage
  • Fatty Acids, Omega-3 / pharmacology
  • Female
  • Horse Diseases / chemically induced
  • Horse Diseases / diet therapy
  • Horses
  • Interleukin-1beta / administration & dosage
  • Interleukin-1beta / toxicity
  • Recombinant Proteins
  • Synovitis / chemically induced
  • Synovitis / diet therapy
  • Synovitis / veterinary

Citations

This article has been cited 8 times.
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