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Acta veterinaria Scandinavica2010; 52(1); 66; doi: 10.1186/1751-0147-52-66

Effect of a povidone-iodine intrauterine infusion on progesterone levels and endometrial steroid receptor expression in mares.

Abstract: Intrauterine infusions have been widely used for the treatment of endometritis in the mare. Nevertheless, their consequences on endocrine and endometrial molecular aspects are unknown. We studied the effect of a 1% povidone-iodine solution intrauterine infusion on progesterone levels, endometrial histology and estrogen (ERα) and progesterone (PR) receptor distribution by immunohistochemistry. Methods: Fourteen healthy mares were used in this study. Estruses were synchronized and seven mares were treated with intrauterine infusions at days 0 and 2 post ovulation of two consecutive estrous cycles. Uterine biopsy samples were taken on days 6 and 15 post ovulation. Results: The treatment did not induce an inflammatory response indicating endometritis, neither affected the ERα. However, it reduced the percentage of PR positive cells (PPC) on day 6 (deep glandular epithelium, control: 95.7 vs. infused: 61.5, P < 0.05). Treated mares tended to have lower progesterone levels on day 2 (3.9 ng/ml vs. 6.6 ng/ml, P = 0.07), and higher levels on day 15 compared with controls (4.4 ng/ml vs. 1.3 ng/ml, P = 0.07). Conclusions: a 1% povidone-iodine infusion during days 0 and 2 post ovulation in healthy mares did not induce histological changes indicating endometritis, but altered progesterone concentrations and reduced the expression of endometrial PR at day 6 without affecting the ERα. These changes could reduce embryo survival.
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Publication Date: 2010-12-16 PubMed ID: 21162724PubMed Central: PMC3017522DOI: 10.1186/1751-0147-52-66Google Scholar: Lookup
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Summary

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The research evaluates the influence of intrauterine infusions using a 1% povidone-iodine solution on progesterone levels, estrogen and progesterone receptor distribution, and endometrial histology in mares but concludes it potentially reduces embryo survival due to altered concentrations of progesterone and a reduction in the expression of endometrial progesterone receptors.

Introduction

The article begins by acknowledging that intrauterine infusions are regularly utilized for endometritis treatment in mares, but the effects on the molecular and endocrine aspects of the endometrium have not been comprehensively studied. The main purpose of this study was to evaluate the effect of a 1% povidone-iodine solution when used in an intrauterine infusion.

Methodology

  • The study involved fourteen healthy mares. Their estruses were synchronized for consistency.
  • Half of the mares were treated with the povidone-iodine intrauterine infusions on the first and second days after ovulation for two consecutive estrous cycles.
  • Uterine biopsy samples from the mares were taken on the sixth and fifteenth days after ovulation.

Results

  • The treatment did not instigate an inflammatory response, indicating no onset of endometritis and no effect on the estrogen receptors.
  • The percentage of progesterone receptor positive cells in the deep glandular epithelium was seen to reduce on the sixth day from 95.7% in control mares to 61.5% in infused mares.
  • Progesterone levels in treated mares trended lower on day 2 (3.9 ng/ml vs. 6.6 ng/ml) and higher on day 15 (4.4 ng/ml vs. 1.3 ng/ml) compared to controls.

Conclusion

The researchers concluded that a 1% povidone-iodine infusion post-ovulation in healthy mares did not induce significant histological changes indicative of endometritis. However, it did affect progesterone concentrations and reduced the expression of endometrial progesterone at day 6 without affecting the estrogen receptors. These alterations potentially reduce embryo survival.

Cite This Article

APA
Kalpokas I, Perdigón F, Rivero R, Talmon M, Sartore I, Viñoles C. (2010). Effect of a povidone-iodine intrauterine infusion on progesterone levels and endometrial steroid receptor expression in mares. Acta Vet Scand, 52(1), 66. https://doi.org/10.1186/1751-0147-52-66

Publication

Acta veterinaria Scandinavica
ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 52
Issue: 1
Pages: 66

Researcher Affiliations

Kalpokas, Irene
  • Faculty of Veterinary Medicine, Uruguay. ikalpokas@hotmail.com
Perdigón, Fernando
    Rivero, Rodolfo
      Talmon, Marilina
        Sartore, Isabel
          Viñoles, Carolina

            MeSH Terms

            • Animals
            • Anti-Infective Agents, Local / administration & dosage
            • Biopsy / veterinary
            • Drug Administration Routes / veterinary
            • Endometrium / drug effects
            • Endometrium / metabolism
            • Estrogen Receptor alpha / blood
            • Estrogen Receptor alpha / metabolism
            • Female
            • Horses / blood
            • Horses / metabolism
            • Immunohistochemistry / veterinary
            • Povidone-Iodine / administration & dosage
            • Progesterone / blood
            • Progesterone / metabolism
            • Receptors, Progesterone / blood
            • Receptors, Progesterone / metabolism
            • Uterus

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            Citations

            This article has been cited 1 times.
            1. Tyrnenopoulou P, Fthenakis GC. Clinical Aspects of Bacterial Distribution and Antibiotic Resistance in the Reproductive System of Equids. Antibiotics (Basel) 2023 Mar 28;12(4).
              doi: 10.3390/antibiotics12040664pubmed: 37107026google scholar: lookup
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