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Home / Equine Research Bank / Vet Med Int / Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells o...
Veterinary medicine international2021; 2021; 5024905; doi: 10.1155/2021/5024905

Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair.

Abstract: To assess the clinical value and safety of the application of allogeneic equine oral mucosa mesenchymal stromal cells (OM-MSCs) to wounds. Animals. 8 healthy adult horses without front limb skin lesions or musculoskeletal disease. Procedures. Stem cells were isolated from the oral mucosa of a donor horse. Horses were subjected to the creation of eight full-thickness cutaneous wounds, two on each distal forelimb (FL) and two on both sides of the thorax (TH). Each wound was subjected to one out of four treatments: no medication (T1), hyaluronic acid- (HA-) gel containing OM-MSC (T2), HA-gel containing OM-MSC secretome (T3), and HA-gel alone (T4). Gross macroscopic evaluation and laser digital photographic documentation were regularly performed to allow wound assessment including wound surface area. Full-thickness skin punch biopsy was performed at each site before wound induction (D0, normal skin) and after complete wound healing (D62, repaired skin). Results: All wounds healed without adverse effect at D62. Distal limb wounds are slower to heal than body wounds. OM-MSC and its secretome have a positive impact on TH wound contraction. OM-MSC has a positive impact on the contraction and epithelialization of FL wounds. No significant difference between wound sites before and after treatment was noted at histological examination. Conclusion and Clinical Relevance. Using horse cells harvested from oral mucosa is a feasible technique to produce OM-MSC or its secretome. The gel produced by the combination of these biologic components with HA shows a positive impact when applied during the early stage of wound healing.
Copyright © 2021 Paola Di Francesco et al.
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Publication Date: 2021-12-14 PubMed ID: 34950446PubMed Central: PMC8692048DOI: 10.1155/2021/5024905Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the use of allogeneic equine oral mucosa mesenchymal stromal cells and their secretome in the treatment of wounds in horses, identifying promising results for wound contraction and epithelialization, without any notable adverse effects.

Research Methods and Methodology

  • The researchers used healthy adult horses with no front limb skin conditions or musculoskeletal disease for the study.
  • Stem cells were isolated from the oral mucosa of a donor horse. These are specialized cells that have the potential to develop into different cell types in the body.
  • Each horse was subjected to eight full-thickness cutaneous wounds, two on their distal forelimb (FL) and two on both sides of the thorax (TH).
  • Each wound was then subjected to one of four treatments: non-treated (T1), treatment with a hyaluronic acid– (HA-) gel containing oral mucosa mesenchymal stromal cells (OM-MSC; T2), treatment with an HA-gel containing OM-MSC secretome (T3), and HA-gel alone (T4).
  • They undertook macroscopic evaluations and laser digital photographic documentation for wound assessment, including evaluation of the wound surface area. Full-thickness skin punch biopsy was performed at each site before the wound induction (D0, normal skin) and after complete wound healing (D62, repaired skin).

Results

  • All the wounds healed without any adverse effects by D62.
  • Wounds located on the distal limb healed slower than those on the body.
  • OM-MSCs and their secretome had a positive effect on thorax wound contraction.
  • OM-MSCs also positively influenced the contraction and epithelialization of forelimb wounds.
  • No significant difference was observed between the wound sites before and after the treatment upon histological examination.

Conclusions

  • The use of cells harvested from the horse’s oral mucosa to generate oral mucosa mesenchymal stromal cells or its secretome, was found to be a viable technique.
  • The gel created by combining these biological components with HA showed a positive impact when applied in the early stage of wound healing.

Cite This Article

APA
Di Francesco P, Cajon P, Desterke C, Perron Lepage MF, Lataillade JJ, Kadri T, Lepage OM. (2021). Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair. Vet Med Int, 2021, 5024905. https://doi.org/10.1155/2021/5024905

Publication

Veterinary medicine international
ISSN: 2090-8113
NlmUniqueID: 101524203
Country: United States
Language: English
Volume: 2021
Pages: 5024905

Researcher Affiliations

Di Francesco, Paola
  • Unité ICE-Groupe de Recherche en Médecine et Rééducation des Equidés de Sport (GREMERES), Centre for Equine Health, Ecole Nationale Vétérinaire de Lyon, VetAgro Sup, Université de Lyon, Marcy l'Etoile 69280, France.
Cajon, Pauline
  • Stem Cell Vet Therapeutics SAS, Elancourt 78990, France.
Desterke, Christophe
  • INSERM UMR935, University of Medicine Paris Sud 11, Orsay, France.
Perron Lepage, Marie-France
  • Vet Tox Path Consulting, Theizé 69620, France.
Lataillade, Jean-Jacques
  • Institut de Recherche Biomédical des Armées, Unité de Thérapie Cellulaire et Réparation Tissulaire, Brétigny sur Orge 91223, France.
Kadri, Tewfik
  • Stem Cell Vet Therapeutics SAS, Elancourt 78990, France.
Lepage, Olivier M
  • Unité ICE-Groupe de Recherche en Médecine et Rééducation des Equidés de Sport (GREMERES), Centre for Equine Health, Ecole Nationale Vétérinaire de Lyon, VetAgro Sup, Université de Lyon, Marcy l'Etoile 69280, France.

Conflict of Interest Statement

Pauline Cajon and Tewfik Kadri are StemT\'s employees; they produced stem cell-based products but did not participate in the clinical study. The other authors declare that they have no conflicts of interest.

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