Effect of amino acid and glucose administration following exercise on the turnover of muscle protein in the hindlimb femoral region of thoroughbreds.
Abstract: In man, muscle protein synthesis is accelerated by administering amino acids (AA) and glucose (Glu), because increased availability of amino acids and increased insulin secretion, is known to have a protein anabolic effect. However, in the horse, the effect on muscle hypertrophy of such nutrition management following exercise is unknown. Objective: To determine the effect of AA and Glu administration following exercise on muscle protein turnover in horses. We hypothesise that administration of AA and Glu after exercise effects muscle hypertrophy in horses, as already shown in man and other animals. Methods: Measurements of the rate of synthesis (Rs) and rate of degradation (Rd) of muscle protein in the hindlimb femoral region of thoroughbred horses were conducted using the isotope dilution method to assess the differences between the artery and iliac vein. Six adult Thoroughbreds received a continuous infusion of L-[ring-2H5]- phenylalanine during the study, the stable period for plasma isotope concentrations (60 min), resting periods (60 min), treadmill exercise (15 min) and recovery period (240 min). All horses were given 4 solutions (saline [Cont], 10% AA [10-AA], 10% Glu [10-Glu] and a mixture with 10% AA and 10% Glu [10-Mix]) over 120 min after exercise, and the Rs and Rd of muscle protein in the hindlimb measured. Results: The average Rs during the 75-120 min following administration of 10-Mix was significantly greater than for the other solutions (P<0.05). The second most effective solution was 10-AA, and there was no change in Rs after 10-Glu. Conclusions: Administration of AA following exercise accelerated Rs in the hindlimb femoral region, and this effect was enhanced when combined with glucose, because of increasing insulin secretion or a decreased requirement for AA for energy. Conclusions: Further studies are required regarding the effect on muscle hypertrophy of supplementing amino acids and glucose in the feed of exercising horses.
Publication Date: 2007-04-04 PubMed ID: 17402492DOI: 10.1111/j.2042-3306.2006.tb05613.xGoogle Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
Summary
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The study aimed to understand how the administration of amino acids and glucose after exercise affects muscle protein turnover in horses, focusing on the femoral region. The findings suggested that the combination of amino acids and glucose enhances the rate of muscle protein synthesis, potentially aiding muscle development.
Research Objectives and Hypotheses
- The research aimed at understanding the effects of administering amino acids (AA) and glucose (Glu) post-exercise on muscle protein turnover in horses.
- The researchers hypothesized that the administration of AA and Glu after physical activity has an effect on the hypertrophy of their muscles.
Methods
- Six adult thoroughbreds were used in this study, and their rates of synthesis (Rs) and degradation (Rd) of the muscle protein in the hindlimb femoral region were measured.
- These measurements were done using the isotope dilution method, which helped in assessing the differences between the artery and iliac vein.
- The horses received continuous infusion of a certain kind of amino acid (L-[ring-2H5]- phenylalanine) during various phases of the study—stable period, resting periods, exercise period, and recovery phase.
- Post-exercise, the horses were given one of four solutions: a saline solution as a control (Cont), 10% amino acid solution (10-AA), 10% glucose solution (10-Glu), and a mixture of 10% AA and 10% Glu (10-Mix), and their Rs and Rd levels were measured.
Results
- Analysis showed significantly greater average Rs during the 75-120 min following the administration of the 10-Mix solution as compared to the other solutions, suggesting an increased rate of protein synthesis.
- The 10-AA was the second most effective solution, while the 10-Glu did not result in any change in Rs.
Conclusions
- The researchers concluded that administering AA after exercise accelerated Rs in the hindlimb femoral region, and this effect was further enhanced when combined with glucose—possibly due to increased insulin secretion or a decreased requirement for AA for energy.
- The study called for further research to explore the impacts of supplementing feed with AA and Glu on muscle hypertrophy in exercising horses.
Cite This Article
APA
Matsui A, Ohmura H, Asai Y, Takahashi T, Hiraga A, Okamura K, Tokimura H, Sugino T, Obitsu T, Taniguchi K.
(2007).
Effect of amino acid and glucose administration following exercise on the turnover of muscle protein in the hindlimb femoral region of thoroughbreds.
Equine Vet J Suppl(36), 611-616.
https://doi.org/10.1111/j.2042-3306.2006.tb05613.x Publication
Researcher Affiliations
- Equine Science Division, Hidaka Training and Research Center, Japan Racing Association, Urakawa-gun, Hokkaido, Japan.
MeSH Terms
- Amino Acids / administration & dosage
- Amino Acids / metabolism
- Animal Nutritional Physiological Phenomena
- Animals
- Area Under Curve
- Cross-Over Studies
- Dose-Response Relationship, Drug
- Female
- Glucose / administration & dosage
- Glucose / metabolism
- Hindlimb
- Horses / metabolism
- Horses / physiology
- Insulin / metabolism
- Insulin Secretion
- Kinetics
- Male
- Muscle Proteins / metabolism
- Muscle, Skeletal / metabolism
- Nutritional Requirements
- Physical Conditioning, Animal / physiology
Citations
This article has been cited 3 times.- Graham-Thiers PM, Bowen LK. The effect of time of feeding on plasma amino acids during exercise and recovery in horses. Transl Anim Sci 2021 Apr;5(2):txab045.
- DeBoer ML, Martinson KM, Pampusch MS, Hansen AM, Wells SM, Ward C, Hathaway M. Cultured equine satellite cells as a model system to assess leucine stimulated protein synthesis in horse muscle. J Anim Sci 2018 Feb 15;96(1):143-153.
- Martin A, Lepers R, Vasseur M, Julliand S. Effect of high-starch or high-fibre diets on the energy metabolism and physical performance of horses during an 8-week training period. Front Physiol 2023;14:1213032.
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