FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Regen Med / Effect of autologous adipose tissue-derived mesenchymal stem...
Regenerative medicine2014; 9(6); 743-757; doi: 10.2217/rme.14.55

Effect of autologous adipose tissue-derived mesenchymal stem cells on neovascularization of artificial equine tendon lesions.

Abstract: To investigate whether autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) treatment of tendon lesions increases neovascularization during tendon healing. Methods: A standardized surgical model was used to create lesions in both front limb superficial digital flexor tendons (SDFTs) of nine horses. Either AT-MSCs or control substance was injected intralesionally 2 weeks post-surgery. Color Doppler ultrasonography of SDFTs was performed at regular intervals. Horses were euthanized 22 weeks post-treatment and SDFTs were harvested for histology. Results: The color Doppler ultrasonography signal was significantly more extensive at 2 weeks post-treatment and the number of vessels counted on histologic slides was significantly higher at 22 weeks post-treatment in AT-MSC-treated SDFTs. Conclusions: Our findings indicate that AT-MSC treatment has a beneficial effect on neovascularization of healing tendons.
Get Full Text
Publication Date: 2014-11-29 PubMed ID: 25431911DOI: 10.2217/rme.14.55Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the impact of stem cells derived from own fat tissues on the new blood vessel formation in artificial horse tendon injuries and suggests that this treatment method improves the healing process.

Methodology

  • The research team created tendon injuries in both front limbs of nine horses using a standard surgical model for a controlled experiment.
  • Two weeks after the creation of the injuries, the team injected either autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) or a control substance into the injured area.
  • At regular intervals following the AT-MSC or control injection, the researchers conducted Color Doppler ultrasonography. This imaging technique allows for the visualization of the blood flow in the tendons to determine the level of neovascularization, or new blood vessel formation, occurring in the injured area.
  • At 22 weeks post-treatment, the horses were euthanized, and the tendons were harvested for histological examination. This allowed researchers to closely analyze the tissue structure and understand the cellular changes that had taken place as a result of the treatment.

Results

  • Two weeks after the stem cell treatment, the Doppler ultrasonography results indicated a significantly more extensive signal in the treated tendons compared to those that received the control substance. This suggests increased blood flow to the area and subsequent neovascularization.
  • At the end of the study, 22 weeks post-treatment, the histological examination discovered a greater number of vessels in AT-MSC treated tendons compared to the control group.
  • The increased new blood vessel formation in the AT-MSC treated group indicates a more rapid and thorough healing process due to the improved nutrient and oxygen delivery to the injured tendons.

Conclusions

  • The research results indicate that the use of AT-MSC treatment significantly improves the healing process of tendon injuries by enhancing neovascularization.
  • This could potentially lead to improved treatment plans for tendon injuries, using the patient’s own adipose tissue-derived stem cells to encourage healing.
  • As this study was conducted on horses, further research is required to investigate the effectiveness of this approach in other animals and humans.

Cite This Article

APA
Conze P, van Schie HT, van Weeren R, Staszyk C, Conrad S, Skutella T, Hopster K, Rohn K, Stadler P, Geburek F. (2014). Effect of autologous adipose tissue-derived mesenchymal stem cells on neovascularization of artificial equine tendon lesions. Regen Med, 9(6), 743-757. https://doi.org/10.2217/rme.14.55

Publication

Regenerative medicine
ISSN: 1746-076X
NlmUniqueID: 101278116
Country: England
Language: English
Volume: 9
Issue: 6
Pages: 743-757

Researcher Affiliations

Conze, Philipp
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Hannover, Bünteweg 9, 30559 Hannover, Germany.
van Schie, Hans T M
    van Weeren, René
      Staszyk, Carsten
        Conrad, Sabine
          Skutella, Thomas
            Hopster, Klaus
              Rohn, Karl
                Stadler, Peter
                  Geburek, Florian

                    MeSH Terms

                    • Adipose Tissue / cytology
                    • Animals
                    • Cells, Cultured
                    • Horse Diseases / pathology
                    • Horse Diseases / therapy
                    • Horses
                    • Immunoenzyme Techniques
                    • Mesenchymal Stem Cell Transplantation
                    • Mesenchymal Stem Cells / cytology
                    • Neovascularization, Physiologic
                    • Tendon Injuries / pathology
                    • Tendon Injuries / therapy
                    • Tendons / blood supply
                    • Transplantation, Autologous

                    Citations

                    This article has been cited 20 times.
                    1. Senesi L, De Francesco F, Marchesini A, Pangrazi PP, Bertolini M, Riccio V, Riccio M. Efficacy of Adipose-Derived Mesenchymal Stem Cells and Stromal Vascular Fraction Alone and Combined to Biomaterials in Tendinopathy or Tendon Injury: Systematic Review of Current Concepts. Medicina (Kaunas) 2023 Jan 31;59(2).
                      doi: 10.3390/medicina59020273pubmed: 36837474google scholar: lookup
                    2. Hagen A, Niebert S, Brandt VP, Holland H, Melzer M, Wehrend A, Burk J. Functional properties of equine adipose-derived mesenchymal stromal cells cultured with equine platelet lysate. Front Vet Sci 2022;9:890302.
                      doi: 10.3389/fvets.2022.890302pubmed: 36016806google scholar: lookup
                    3. Rezaei F, Khasaf S, Ghasemi S, Parham A, Mirshokraei P. How to maintain and transport equine adipose tissue for isolating mesenchymal stem cells?. BMC Vet Res 2022 Jul 21;18(1):284.
                      doi: 10.1186/s12917-022-03379-1pubmed: 35864533google scholar: lookup
                    4. Song K, Yang GM, Han J, Gil M, Dayem AA, Kim K, Lim KM, Kang GH, Kim S, Jang SB, Vellingiri B, Cho SG. Modulation of Osteogenic Differentiation of Adipose-Derived Stromal Cells by Co-Treatment with 3, 4'-Dihydroxyflavone, U0126, and N-Acetyl Cysteine. Int J Stem Cells 2022 Aug 30;15(3):334-345.
                      doi: 10.15283/ijsc22044pubmed: 35769058google scholar: lookup
                    5. Roth SP, Burk J, Brehm W, Troillet A. MSC in Tendon and Joint Disease: The Context-Sensitive Link Between Targets and Therapeutic Mechanisms. Front Bioeng Biotechnol 2022;10:855095.
                      doi: 10.3389/fbioe.2022.855095pubmed: 35445006google scholar: lookup
                    6. Depuydt E, Broeckx SY, Chiers K, Patruno M, Da Dalt L, Duchateau L, Saunders J, Pille F, Martens A, Van Hecke L, Spaas JH. Cellular and Humoral Immunogenicity Investigation of Single and Repeated Allogeneic Tenogenic Primed Mesenchymal Stem Cell Treatments in Horses Suffering From Tendon Injuries. Front Vet Sci 2021;8:789293.
                      doi: 10.3389/fvets.2021.789293pubmed: 35281431google scholar: lookup
                    7. Depuydt E, Broeckx SY, Van Hecke L, Chiers K, Van Brantegem L, van Schie H, Beerts C, Spaas JH, Pille F, Martens A. The Evaluation of Equine Allogeneic Tenogenic Primed Mesenchymal Stem Cells in a Surgically Induced Superficial Digital Flexor Tendon Lesion Model. Front Vet Sci 2021;8:641441.
                      doi: 10.3389/fvets.2021.641441pubmed: 33748217google scholar: lookup
                    8. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
                      doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
                    9. Jankowski M, Dompe C, Sibiak R, Wąsiatycz G, Mozdziak P, Jaśkowski JM, Antosik P, Kempisty B, Dyszkiewicz-Konwińska M. In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications. Cells 2020 Jul 27;9(8).
                      doi: 10.3390/cells9081783pubmed: 32726947google scholar: lookup
                    10. Lim WL, Liau LL, Ng MH, Chowdhury SR, Law JX. Current Progress in Tendon and Ligament Tissue Engineering. Tissue Eng Regen Med 2019 Dec;16(6):549-571.
                      doi: 10.1007/s13770-019-00196-wpubmed: 31824819google scholar: lookup
                    11. Kornicka K, Geburek F, Röcken M, Marycz K. Stem Cells in Equine Veterinary Practice-Current Trends, Risks, and Perspectives. J Clin Med 2019 May 14;8(5).
                      doi: 10.3390/jcm8050675pubmed: 31091732google scholar: lookup
                    12. Ahrberg AB, Horstmeier C, Berner D, Brehm W, Gittel C, Hillmann A, Josten C, Rossi G, Schubert S, Winter K, Burk J. Effects of mesenchymal stromal cells versus serum on tendon healing in a controlled experimental trial in an equine model. BMC Musculoskelet Disord 2018 Jul 18;19(1):230.
                      doi: 10.1186/s12891-018-2163-ypubmed: 30021608google scholar: lookup
                    13. Geburek F, Roggel F, van Schie HTM, Beineke A, Estrada R, Weber K, Hellige M, Rohn K, Jagodzinski M, Welke B, Hurschler C, Conrad S, Skutella T, van de Lest C, van Weeren R, Stadler PM. Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial. Stem Cell Res Ther 2017 Jun 5;8(1):129.
                      doi: 10.1186/s13287-017-0564-8pubmed: 28583184google scholar: lookup
                    14. Berglund AK, Schnabel LV. Allogeneic major histocompatibility complex-mismatched equine bone marrow-derived mesenchymal stem cells are targeted for death by cytotoxic anti-major histocompatibility complex antibodies. Equine Vet J 2017 Jul;49(4):539-544.
                      doi: 10.1111/evj.12647pubmed: 27862236google scholar: lookup
                    15. Geburek F, Mundle K, Conrad S, Hellige M, Walliser U, van Schie HT, van Weeren R, Skutella T, Stadler PM. Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions--a pilot study. Stem Cell Res Ther 2016 Feb 1;7:21.
                      doi: 10.1186/s13287-016-0281-8pubmed: 26830812google scholar: lookup
                    16. Arnhold S, Wenisch S. Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine. Am J Stem Cells 2015;4(1):1-12.
                      pubmed: 25973326
                    17. Schmitz C, Alt C, Wuerfel T, Milz S, Dinzey J, Hill A, Sikes KJ, Burton LH, Easley J, Stewart HL, Puttlitz CM, Gadomski BC, Labus KM, Pearce DA, Maffulli N, Alt EU. Regeneration of Biomechanically Functional Tendon Tissue Following Injection of Uncultured, Autologous, Adipose-Derived Regenerative Cells into Partial Achilles Tendon Defects in Rabbits. Int J Mol Sci 2025 Jul 16;26(14).
                      doi: 10.3390/ijms26146800pubmed: 40725049google scholar: lookup
                    18. Reis IL, Lopes B, Sousa P, Sousa AC, Caseiro AR, Mendonça CM, Santos JM, Atayde LM, Alvites RD, Maurício AC. Equine Musculoskeletal Pathologies: Clinical Approaches and Therapeutical Perspectives-A Review. Vet Sci 2024 Apr 26;11(5).
                      doi: 10.3390/vetsci11050190pubmed: 38787162google scholar: lookup
                    19. Burk J, Wittenberg-Voges L, Schubert S, Horstmeier C, Brehm W, Geburek F. Treatment of Naturally Occurring Tendon Disease with Allogeneic Multipotent Mesenchymal Stromal Cells: A Randomized, Controlled, Triple-Blinded Pilot Study in Horses. Cells 2023 Oct 24;12(21).
                      doi: 10.3390/cells12212513pubmed: 37947591google scholar: lookup
                    20. Citro V, Clerici M, Boccaccini AR, Della Porta G, Maffulli N, Forsyth NR. Tendon tissue engineering: An overview of biologics to promote tendon healing and repair. J Tissue Eng 2023 Jan-Dec;14:20417314231196275.
                      doi: 10.1177/20417314231196275pubmed: 37719308google scholar: lookup
                    Subscribe to our newsletter
                    Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.