Effect of calcium and fatty acids on the isolation of stallion spermatozoa in BSA.
Abstract: Fifty ejaculates, ten from each of 5 mature stallions, were utilized to study the effects of calcium and fatty acids on equine spermatozoa which were isolated in 3% Bovine Serum Albumin (BSA). The ejaculates were evaluated for percent motile spermatozoa, rate of forward movement, debris, primary abnormalities and secondary abnormalities. The isolation procedure consisted of layering 2 ml of diluted semen (100 x 10(6) spermatozoa/ml) over 6 ml of 3% BSA in 13 x 125 mm columns in a water bath (37 degrees C). After 30 min., the top semen layer and upper half of the BSA layer were withdrawn from all columns and the lower half of the BSA was re-evaluated. A 2 x 2 factorial arrangement of treatments was utilized with either the inclusion or omission of calcium or fatty acids in the BSA isolation media. The percent motile spermatozoa and rate of forward movement were increased (P<.01) when fatty acids were included in the isolation media but decreased (P<.01) when they were omitted. The highest percent motile spermatozoa and rate of forward movement were observed with BSA in the presence of fatty acids and omission of calcium. The calcium by fatty acid interaction, stallion effect and stallion by treatment interaction were significant for percent motile spermatozoa. Less debris was observed in all samples of isolated spermatozoa when compared with the initial estimate. Isolation resulted in a reduction of (P<.01) the primary abnormalities. Also, fewer (P<.01) secondary abnormalities were observed after isolation in all treatments except 4 (-FA+Ca) than were found in the ejaculate sample.
Publication Date: 1981-04-01 PubMed ID: 16725593DOI: 10.1016/0093-691x(81)90001-7Google Scholar: Lookup
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- Journal Article
Summary
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The research paper is about a study demonstrating the effects of calcium and fatty acids on equine spermatozoa isolated within 3% Bovine Serum Albumin (BSA). The results suggest that the presence of fatty acids and the absence of calcium enhance the movement and motility of stallion spermatozoa.
Methodology
- The research was conducted with fifty ejaculates obtained from five mature stallions, resulting in ten samples from each.
- The spermatozoa in each sample were evaluated on five parameters: motility, rate of forward movement, debris, primary abnormalities and secondary abnormalities.
- Isolation took place inside a 3% BSA solution, with 2 ml of diluted semen layered over 6 ml of this BSA solution. A water bath at 37 degrees Celsius was used to conduct this isolation.
- After a duration of thirty minutes, the upper half of the semen and BSA solution was removed, and the remaining BSA and semen sample was evaluated further.
Treatment Method
- A 2 x 2 factorial arrangement of treatments was employed, in which the presence or absence of calcium and fatty acids were manipulated within the isolation media of BSA.
- The researchers examined the effects these manipulations had on the motile spermatozoa and their forward movement.
Results
- The findings highlight how sperm motility and forward movement were enhanced with the inclusion of fatty acids.
- These same parameters were decreased when fatty acids were absent.
- Conversely, an absence of calcium resulted in an increase in spermatozoa motility and forward movement.
- Notably, the most significant improvements in motility and forward movement were observed when the BSA solution contained fatty acids and lacked calcium.
- The study also found that the inclusion of calcium and fatty acids in the BSA significantly influenced the percentage of motile spermatozoa, constituting a significant interaction.
- The isolation process also led to a reduction in the debris, primary abnormalities and secondary abnormalities found among isolated sperm samples.
Conclusion
- The results indicate significant potential for the optimization of stallion sperm isolation methods, helping to reduce abnormalities and increase sperm motility and forward movement.
- This could have significant impacts in the fields of equine reproductive biology and veterinary medicine.
Cite This Article
APA
Dixon KE, Kreider JL.
(1981).
Effect of calcium and fatty acids on the isolation of stallion spermatozoa in BSA.
Theriogenology, 15(4), 335-344.
https://doi.org/10.1016/0093-691x(81)90001-7 Publication
Researcher Affiliations
- Louisiana State University Baton Rouge, LA 70803 USA.
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