Effect of centrifugation and seminal plasma on motility and fertility of stallion and bull spermatozoa.
Abstract: The effect of centrifugation of diluted and undiluted semen on equine and bovine spermatozoan motility and fertility was examined, as was the effect of seminal plasma and dilution on stallion spermatozoa during incubation before and after freezing. Centrifugation at 370 g or 829 g was not detrimental (P greater than 0.05) to prefreeze or postfreeze motility if a final concentration of 10% seminal plasma was present. A reduction of seminal plasma from 10% to 2% significantly (P smaller than 0.05) reduced motility. A centrifugal force of 956 g significantly reduced prefreeze but not postfreeze motility of spermatozoa in undiluted semen, regardless of seminal plasma concentration. With a dried skim milk extender, prefreeze and postfreeze motility was greater in samples containing 20% seminal plasma. Motility was depressed by high and low concentrations of seminal plasma. The fertility of frozen or unfrozen stallion spermatozoa was not depressed (P greater than 0.05) by centrifugation at 310 g for 3.5 minutes. In contrast, the fertility of bull semen was significantly (P smaller than 0.05) lowered by centrifugation at 270 g for three minutes. Further, the fertility of centrifuged, diluted bovine semen was lower (P smaller than 0.05) than centrifuged, undiluted semen.
Publication Date: 1975-02-01 PubMed ID: 1126461DOI: 10.1016/s0015-0282(16)40938-6Google Scholar: Lookup
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- Journal Article
Summary
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The study investigates the impact of centrifugation and seminal plasma on the motility and fertility of bull and stallion sperm, confirming that centrifugation had no negative impact on stallion sperm but reduced the fertility of bull sperm.
Research Methodology
- The researchers studied the effects of centrifugation on both undiluted and diluted semen taken from both horses and cattle. They also studied the effects of seminal plasma and dilution on stallion sperm at the time of incubation prior and post freezing.
- The centrifugation was conducted at varying forces (370 g, 829 g, and 956 g) to determine if the process impacts sperm motility.
Research Findings
- It was found that centrifugation at both 370 g and 829 g did not have any conclusive negative effect on prefreeze or postfreeze motility in samples which maintained a seminal plasma concentration of 10%.
- When the seminal plasma concentration was reduced from 10% to 2%, a significant decrease in motility was noticed.
- A centrifugation force of 956 g was observed to decrease prefreeze motility in undiluted samples, but did not negatively impact the postfreeze motility, regardless of the seminal plasma concentration.
- Using a dried skim milk extender, researchers found that both prefreeze and postfreeze motility was higher in samples that contained 20% seminal plasma. However, both high and low concentrations of seminal plasma were found to depress motility.
- The research found that under the centrifugal force of 310 g for 3.5 minutes, the fertility of both frozen and unfrozen stallion sperm was unaffected. Conversely, the fertility of bull semen was significantly reduced when centrifuged at 270 g for three minutes.
- It was also noted that the fertility of diluted bull semen that had been centrifuged was lower than that of undiluted bull semen that had been centrifuged.
Research Implications
- The results of the study suggest that while the process of centrifugation does not significantly affect the fertility or motility of stallion sperm, it does negatively impact the fertility of bull sperm.
- The findings offer important guidance in the development of sperm handling and freezing protocols for bulls, in order to prevent degradation in fertility.
Cite This Article
APA
Pickett BW, Sullivan JJ, Byers WW, Pace MM, Remmenga EE.
(1975).
Effect of centrifugation and seminal plasma on motility and fertility of stallion and bull spermatozoa.
Fertil Steril, 26(2), 167-174.
https://doi.org/10.1016/s0015-0282(16)40938-6 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Cattle
- Cell Movement
- Centrifugation
- Fertility
- Freezing
- Horses
- Insemination, Artificial / veterinary
- Male
- Semen
- Spermatozoa / physiology
Citations
This article has been cited 9 times.- Sugai N, Werre S, Cecere J, Balogh O. Defining an Optimal Range of Centrifugation Parameters for Canine Semen Processing. Animals (Basel) 2023 Apr 21;13(8).
- Neila-Montero M, Riesco MF, Alvarez M, Montes-Garrido R, Boixo JC, de Paz P, Anel-Lopez L, Anel L. Centrifugal force assessment in ram sperm: identifying species-specific impact. Acta Vet Scand 2021 Nov 4;63(1):42.
- Umair M, Henning H, Stout TAE, Claes A. A Modified Flotation Density Gradient Centrifugation Technique Improves the Semen Quality of Stallions with a High DNA Fragmentation Index. Animals (Basel) 2021 Jul 1;11(7).
- Castiglione Morelli MA, Ostuni A, Giangaspero B, Cecchini S, Carluccio A, Boni R. Relationships between Seminal Plasma Metabolites, Semen Characteristics and Sperm Kinetics in Donkey (Equus asinus). Animals (Basel) 2021 Jan 15;11(1).
- Marzano G, Moscatelli N, Di Giacomo M, Martino NA, Lacalandra GM, Dell'Aquila ME, Maruccio G, Primiceri E, Chiriacò MS, Zara V, Ferramosca A. Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm. Biology (Basel) 2020 Jan 27;9(2).
- Gloria A, Carluccio A, Wegher L, Robbe D, Befacchia G, Contri A. Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates. J Anim Sci Biotechnol 2016;7:30.
- Heiskanen ML, Huhtinen M, Pirhonen A, Mäenpää PH. Insemination results with slow-cooled stallion semen stored for approximately 40 hours. Acta Vet Scand 1994;35(3):257-62.
- Heiskanen ML, Hilden L, Hyyppä S, Kangasniemi A, Pirhonen A, Mäenpää PH. Freezability and fertility results with uncentrifuged stallion semen. Acta Vet Scand 1994;35(4):377-82.
- Sinagra L, Polisca A, Donato G, Caspanello T, Pettina G, Pastore S, De Majo M, Cristarella S, Quartuccio M, Zappone V. Enhancing canine semen quality through a second centrifugation after 48 hours of storage: a comparative study. Acta Vet Scand 2024 Sep 11;66(1):47.
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