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Home / Equine Research Bank / J Vet Intern Med / Effect of Delayed Digital Hypothermia on Lamellar Inflammato...
Journal of veterinary internal medicine2017; 31(2); 575-581; doi: 10.1111/jvim.14633

Effect of Delayed Digital Hypothermia on Lamellar Inflammatory Signaling in the Oligofructose Laminitis Model.

Abstract: In the oligofructose (OF) model of sepsis-related laminitis (SRL), digital hypothermia ("cryotherapy") initiated before the onset of clinical signs is reported not only to limit lamellar injury, but also to cause marked inhibition of lamellar inflammatory signaling. Objective: Because hypothermia also has been reported to be protective when not initiated until the onset of lameness in the OF model of SRL, we hypothesized that the lamellar protection conferred by hypothermia is caused by local lamellar inhibition of inflammatory signaling as described when hypothermia was initiated earlier in the disease process. Methods: Eight Standardbred geldings aged 3-11 years with no lameness and no abnormalities of the feet detectable by gross or radiographic examination. Methods: Using the OF model of SRL, lamellar mRNA concentrations of proinflammatory cytokines, chemokines, and endothelial adhesion proteins were compared between samples from treated limbs (CRYO, submerged in ice water for 36 hour starting at the onset of lameness), untreated limbs (NON-CRYO, opposite limb from CRYO limbs maintained at ambient temperature), and untreated limbs from normal horses in which laminitis was not induced (CON). Results: Although OF administration resulted in increases in lamellar mRNA concentrations of several inflammatory mediators in NON-CRYO limbs (vs CON), digital hypothermia had no significant effect on these increases. Conclusions: The lack of inflammatory inhibition in lamellar tissue samples in our study indicates that the protective effects of digital hypothermia instituted at the onset of clinical signs of laminitis do not arise from inhibition of inflammatory pathways.
Copyright © 2017 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2017-02-01 PubMed ID: 28145603PubMed Central: PMC5354059DOI: 10.1111/jvim.14633Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the effects of delayed digital hypothermia on lamellar inflammatory signaling in horses suffering from sepsis-related laminitis. The findings indicate that the protective effects of digital hypothermia do not derive from inhibiting inflammatory pathways.

Objective and Hypothesis

  • The intent of the research was to explore if the protective effect of hypothermia on horses suffering from sepsis-related laminitis, instigated in the same timeframe as the onset of clinical signs, was due to local inflammatory signalling in the lamellae.
  • The researchers hypothesized that the lamellar protection provided by hypothermia originates from local lamellar inhibition of inflammatory signaling, similar to earlier initiation of hypothermia in the disease process.

Methods

  • The study was performed using eight healthy standardbred gelding horses, aged between 3-11 years, with no noticeable lameness or abnormalities in their feet.
  • The oligofructose model of sepsis-related laminitis was employed. The lamellar mRNA concentrations of chemokines, endothelial adhesion proteins, and proinflammatory cytokines were compared.
  • The comparisons included samples from limbs treated with digital hypothermia (CRYO), untreated limbs (NON-CRYO), and untreated limbs of healthy horses where laminitis was not induced (CON).
  • The treatment for CRYO limbs was submersion in ice water for 36 hours starting at the onset of lameness.

Results

  • The process of administering oligofructose led to an increase in lamellar mRNA concentrations of multiple inflammatory mediators in the NON-CRYO limbs when compared to the CON limbs.
  • It was found, interestingly, that digital hypothermia did not significantly affect these increases.

Conclusions

  • The results imply that the protective influence of digital hypothermia in cases of clinical signs of laminitis does not come from blocking inflammatory pathways.
  • According to the tissue samples studied, there was no significant inflammatory inhibition in lamellar tissue.

Cite This Article

APA
Dern K, Watts M, Werle B, van Eps A, Pollitt C, Belknap J. (2017). Effect of Delayed Digital Hypothermia on Lamellar Inflammatory Signaling in the Oligofructose Laminitis Model. J Vet Intern Med, 31(2), 575-581. https://doi.org/10.1111/jvim.14633

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 31
Issue: 2
Pages: 575-581

Researcher Affiliations

Dern, K
  • Department of Veterinary Clinical Sciences, Ohio State University, Columbus, OH.
Watts, M
  • Department of Veterinary Clinical Sciences, Ohio State University, Columbus, OH.
Werle, B
  • Department of Veterinary Clinical Sciences, Ohio State University, Columbus, OH.
van Eps, A
  • Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Gatton, Qld, Australia.
Pollitt, C
  • Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Gatton, Qld, Australia.
Belknap, J
  • Department of Veterinary Clinical Sciences, Ohio State University, Columbus, OH.

MeSH Terms

  • Animals
  • Foot Diseases / etiology
  • Foot Diseases / pathology
  • Foot Diseases / therapy
  • Foot Diseases / veterinary
  • Hoof and Claw / pathology
  • Horse Diseases / etiology
  • Horse Diseases / pathology
  • Horses
  • Hypothermia, Induced / veterinary
  • Inflammation / etiology
  • Inflammation / pathology
  • Inflammation / therapy
  • Inflammation / veterinary
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Lameness, Animal
  • Male
  • Oligosaccharides / administration & dosage
  • Oligosaccharides / toxicity
  • Real-Time Polymerase Chain Reaction

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Citations

This article has been cited 7 times.
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