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Animals : an open access journal from MDPI2020; 10(12); doi: 10.3390/ani10122244

Effect of Dietary Flaxseed Oil Supplementation on the Redox Status, Haematological and Biochemical Parameters of Horses’ Blood.

Abstract: This study compared the effect of two dietary vegetable oils on plasma biochemical indices, haematological parameters, and redox status of horses. Forty riding horses (20 mares and 20 stallions) of the Malopolski breed were divided equally into two groups that were similar in terms of age, sex, and body weight (on average 530 ± 30 kg). The horses received soybean oil (SO) or flaxseed oil (FO) in the amount of 25 mL per 100 kg BW/day. After 60 days, blood was collected for biochemical and haematological analyses. The results show that horses receiving FO as compared to the SO group had significantly lower plasma levels of glucose, low density lipoprotein cholesterol, total cholesterol/high-density lipoprotein (HDL) ratio and triacylglycerols, as well as the activities of alanine aminotransferase and alkaline phosphatase. In turn, %HDL-TC and lactate dehydrogenase activity were significantly higher in the FO group. The inclusion of FO in the diet contributed to an increase in antioxidant indices: creatinine, vitamin C, copper, and zinc contents and also superoxide dismutase and catalase activities. The level of the end product of lipid peroxidation, i.e., malonyl dialdehyde, in the FO group as compared to the SO group was significantly lower. Moreover, FO caused an elevation in red blood cell indicators, lymphocyte count and lysozymes. In conclusion, FO exerts a beneficial effect by stimulating antioxidant defence mechanisms of horses and reducing the severity of oxidative stress. FO also improved the lipid profile and haematological parameters of the blood. The replacement of SO by FO is recommended based on these findings.
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Publication Date: 2020-11-30 PubMed ID: 33265987PubMed Central: PMC7760300DOI: 10.3390/ani10122244Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how dietary flaxseed oil improves haematological parameters and antioxidant mechanisms on a cellular level in horses, reducing oxidative stress, in contrast to soybean oil.

Methodology

  • The research involved an experimental setup with forty riding horses, divided equally into two groups. These groups were equivalent in age, gender, and body weight.
  • The horses were fed with either soybean oil (SO) or flaxseed oil (FO) in specific quantities.
  • Blood samples were collected after a period of 60 days for detailed biochemical and haematological analyses.

Findings

  • The results revealed horses fed with flaxseed oil had significantly lower plasma levels of glucose, low-density lipoprotein cholesterol, the total cholesterol/high-density lipoprotein ratio, and triacylglycerols. They also registered lower activities of alanine aminotransferase and alkaline phosphatase, which are enzymes related to liver health.
  • In contrast, the said group had remarkably higher levels of HDL-TC (good cholesterol) and lactate dehydrogenase activity compared to the group fed soybean oil.
  • Dietary flaxseed oil also contributed to an increase in antioxidant indices. These include creatinine, vitamin C, copper, and zinc contents, along with superoxide dismutase and catalase activity – enzymes that help in protection against oxidative damage.
  • The level of malonyl dialdehyde, an end product of lipid peroxidation and a marker of oxidative stress, was significantly low in the FO group. This is noteworthy as it emphasizes less oxidative stress in this group.
  • Moreover, flaxseed oil supplementation caused an elevation in red blood cell indicators, lymphocyte count and lysozymes. These changes point towards improved immune responses.

Conclusion

  • The conclusion of the research article suggests that flaxseed oil exerts a beneficial effect by stimulating horses’ antioxidant defense mechanisms and reducing oxidative stress severity.
  • It also improved the lipid profile and haematological parameters of the blood, which can contribute to better overall health for the subject animals.
  • The study recommends the replacement of soybean oil by flaxseed oil in horses’ diet based on these positive findings.

Cite This Article

APA
Sembratowicz I, Zięba G, Cholewinska E, Czech A. (2020). Effect of Dietary Flaxseed Oil Supplementation on the Redox Status, Haematological and Biochemical Parameters of Horses’ Blood. Animals (Basel), 10(12). https://doi.org/10.3390/ani10122244

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 10
Issue: 12

Researcher Affiliations

Sembratowicz, Iwona
  • Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
Zięba, Grzegorz
  • Department of Biological Basis of Animal Production, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
Cholewinska, Ewelina
  • Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
Czech, Anna
  • Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.

Conflict of Interest Statement

The authors declare no conflict of interest.

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