Effect of emptying the vasculature before performing regional limb perfusion with amikacin in horses.
Abstract: Emptying the vasculature with an Esmarch bandage before i.v. regional anaesthesia is commonly performed in human patients to prevent leakage of the solution under the tourniquet but there is no evidence for its efficacy in horses for antimicrobial i.v. regional limb perfusion (IV-RLP). Objective: To determine the effect on synovial fluid concentration of amikacin of emptying the vasculature before performing IV-RLP. Methods: Crossover experiment. Methods: Eight clinically healthy horses underwent 2 IV-RLP with amikacin in a randomised, crossover design. Horses received an IV-RLP with amikacin with or without exsanguination before applying a pneumatic tourniquet at the level of the forearm. Blood was collected from the jugular vein (before tourniquet removal) and synovial fluid from the radiocarpal and metacarpophalangeal joints (5 min after tourniquet removal and at 24 h) for amikacin determination. The procedure was video recorded to assess horse movement. Results: There was no difference in amikacin concentrations in the plasma or synovial fluid from the radiocarpal joint between groups. There was a higher concentration of amikacin in the synovial fluid from the metacarpophalangeal joint immediately after tourniquet removal in the group with exsanguination of the limb prior to IV-RLP (mean ± s.d.: no exsanguination 49.7 ± 53.7 μg/ml, exsanguination 257.4 ± 149.7 μg/ml, P = 0.04). Horse movement did not differ significantly between groups. Conclusions: Emptying the vasculature with an Esmarch bandage before IV-RLP can improve amikacin concentrations in the metacarpophalangeal joint of horses with no effect on the levels of amikacin in the radiocarpal joint.
© 2015 EVJ Ltd.
Publication Date: 2015-10-06 PubMed ID: 26278891DOI: 10.1111/evj.12501Google Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
- Antibiotics
- Clinical Examination
- Clinical Findings
- Clinical Pathology
- Clinical Study
- Clinical Symptoms
- Diagnosis
- Disease control
- Disease Diagnosis
- Disease Management
- Disease Treatment
- Equine Health
- Horses
- In Vivo
- Pharmacodynamics
- Pharmacokinetics
- Pharmacology
- Veterinary Care
- Veterinary Medicine
- Veterinary Practice
- Veterinary Procedure
Summary
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The research article examines the impact of removing blood from the vasculature before performing intravenous regional limb perfusion (IV-RLP) with amikacin in horses. The findings show that this procedure can improve amikacin concentration in the horse’s metacarpophalangeal joint without affecting the levels in the radiocarpal joint.
Objective and Methodology
- The objective of this study was to assess the effect of emptying the vasculature using an Esmarch bandage before the administration of IV-RLP on synovial fluid concentration of amikacin in horses.
- The research involved a crossover experiment with eight clinically healthy horses. The horses underwent two IV-RLP procedures with amikacin in a randomised, crossover design. These were performed either with or without blood removal before applying a pneumatic tourniquet at the level of the forearm.
- Sample collections were made from the jugular vein before removing the tourniquet, and from the synovial fluid in the radiocarpal and metacarpophalangeal joints 5 minutes after tourniquet removal and at 24 hours post-operation for amikacin determination.
- The entire procedure was video recorded for the purpose of assessing horse movement during the experiment.
Results
- The results showed no difference in amikacin concentrations in the plasma or synovial fluid drawn from the radiocarpal joint between the groups.
- However, there was a significantly higher concentration of amikacin in the synovial fluid extracted from the metacarpophalangeal joint right after tourniquet removal in the group where blood removal was carried out prior to IV-RLP.
- The movement of the horses did not vary significantly between the two groups.
Conclusions
- The study concluded that removing blood from the vasculature using an Esmarch bandage before the IV-RLP procedure can enhance the concentration of amikacin in the horse’s metacarpophalangeal joint.
- This manipulation does not influence the levels of amikacin in the radiocarpal joint.
Cite This Article
APA
Sole A, Nieto JE, Aristizabal FA, Snyder JR.
(2015).
Effect of emptying the vasculature before performing regional limb perfusion with amikacin in horses.
Equine Vet J, 48(6), 737-740.
https://doi.org/10.1111/evj.12501 Publication
Researcher Affiliations
- William R. Pritchard Veterinary Medical Teaching Hospital, University of California, Davis, USA. albert.soleguitart@sydney.edu.au.
- Departments of Surgical and Radiological Sciences, University of California, Davis, USA.
- William R. Pritchard Veterinary Medical Teaching Hospital, University of California, Davis, USA.
- Departments of Surgical and Radiological Sciences, University of California, Davis, USA.
MeSH Terms
- Amikacin / administration & dosage
- Amikacin / pharmacokinetics
- Animals
- Anti-Bacterial Agents / administration & dosage
- Anti-Bacterial Agents / pharmacokinetics
- Cross-Over Studies
- Forelimb / blood supply
- Horses
- Regional Blood Flow
- Tissue Distribution
Citations
This article has been cited 3 times.- Bonilla AG, Causeret L, Torrent-Crosa A. Pharmacokinetics of ceftiofur in the metacarpophalangeal joint after standing intravenous regional limb perfusion in horses. Can Vet J 2021 Sep;62(9):975-981.
- Celani G, Tulini SMR, Montesano C, Zezza D, Sergi M, Varasano V, Mortellaro CM, Compagnone D, Amorena M, Petrizzi L. Pharmacokinetics of marbofloxacin administered via intravenous regional limb perfusion in dairy cows: evaluation of two different tourniquets. Vet Rec Open 2017;4(1):e000227.
- Nieto JE, Trela J, Stanley SD, Yamout S, Snyder JR. Pharmacokinetics of a combination of amikacin sulfate and penicillin G sodium for intravenous regional limb perfusion in adult horses. Can J Vet Res 2016 Jul;80(3):230-5.
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