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Home / Equine Research Bank / Anim Reprod Sci / Effect of extender and amino acid supplementation on sperm q...
Animal reproduction science2014; 146(1-2); 79-88; doi: 10.1016/j.anireprosci.2014.02.009

Effect of extender and amino acid supplementation on sperm quality of cooled-preserved Andalusian donkey (Equus asinus) spermatozoa.

Abstract: The main aim of this study was to evaluate the efficacy of two commercially available liquid stallion semen extenders for the preservation of Andalusian donkey semen at 5°C for up to 72h, and to evaluate the effect of amino acid addition on sperm quality of cooled donkey semen. In addition, this study investigated the effect of seasons on semen characteristics of Andalusian jackasses. Throughout a year, 50 ejaculates were collected from ten adult donkeys and a complete semen evaluation was performed immediately after collection. In Experiment 1, semen samples (n=32) were pooled, divided into two aliquots, and cooled in either Gent(®) A or INRA 96(®). In Experiment 2, pooled semen samples (n=9) were cooled in Gent A(®) supplemented with 0 (as control), 20, 40, or 60mM for each glutamine, proline, or taurine. Fresh semen and chilled samples were assessed for sperm motility, morphology, acrosome integrity, and plasma membrane integrity. Sperm motility variables were greater (P<0.05) in Gent(®) A than in INRA 96(®). The presence of glutamine, proline, or taurine in Gent(®) A improved (P<0.001) the motility of Andalusian donkey spermatozoa. Differences (P<0.05) in some sperm variables were observed among seasons. In conclusion, Gent(®) A maintained sperm motility characteristics after 72h of cold storage to a greater extent than INRA 96(®). Moreover, motility was greater when Gent(®) A supplemented at different concentrations of amino acids than Gent(®) A with no supplementation. An effect of seasons on the semen quality of the Andalusian donkey was demonstrated.
Copyright © 2014 Elsevier B.V. All rights reserved.
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Publication Date: 2014-02-19 PubMed ID: 24602506DOI: 10.1016/j.anireprosci.2014.02.009Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how different semen preservation methods and amino acid supplements affect the quality of Andalusian donkey sperm, kept at 5°C for up to 72 hours. The researchers also examine the impact of different seasons on the quality of the species’ sperm.

Methodology

  • The researchers gathered 50 ejaculates from ten adult Andalusian donkeys throughout a year.
  • A comprehensive evaluation of each semen sample was conducted immediately after collection.
  • In the first experiment, 32 semen samples were pooled, divided into two, and preserved in two commercially available stallion semen extenders: Gent® A or INRA 96®.
  • In the second experiment, nine pooled semen samples were chilled in Gent A®. Each sample was supplemented with 0 (as a control), 20, 40, or 60mM of either glutamine, proline, or taurine, which are all amino acids.
  • The researchers then assessed the fresh semen and preserved samples for sperm motility, morphology, acrosome integrity, and plasma membrane integrity.

Findings

  • It was found that sperm motility variables were greater in Gent® A than in INRA 96®. This indicates that Gent® A is more effective in preserving sperm motility over a 72-hour cooling period.
  • The inclusion of glutamine, proline, or taurine in Gent® A was seen to enhance the motility of the donkey sperm significantly.
  • There were noticeable variations in some sperm variables across different seasons, demonstrating seasonal impacts on semen quality.

Conclusions

  • The research concluded that Gent® A maintained sperm motility characteristics beyond those preserved in INRA 96® after a 72-hour cooling period.
  • Furthermore, semen motility was improved when Gent® A was supplemented with different concentrations of amino acids as opposed to Gent® A without supplementation.
  • A clear effect of seasons on Andalusian donkey semen quality was established in this study, with variations in quality observed across different periods of the year.

These findings suggest potentially improved methods for the preservation of Andalusian donkey semen that can positively influence its quality.

Cite This Article

APA
Dorado J, Acha D, Ortiz I, Gálvez MJ, Carrasco JJ, Gómez-Arrones V, Calero-Carretero R, Hidalgo M. (2014). Effect of extender and amino acid supplementation on sperm quality of cooled-preserved Andalusian donkey (Equus asinus) spermatozoa. Anim Reprod Sci, 146(1-2), 79-88. https://doi.org/10.1016/j.anireprosci.2014.02.009

Publication

Animal reproduction science
ISSN: 1873-2232
NlmUniqueID: 7807205
Country: Netherlands
Language: English
Volume: 146
Issue: 1-2
Pages: 79-88

Researcher Affiliations

Dorado, J
  • Animal Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, 14071 Córdoba, Spain. Electronic address: jdorado@uco.es.
Acha, D
  • Animal Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, 14071 Córdoba, Spain.
Ortiz, I
  • Animal Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, 14071 Córdoba, Spain.
Gálvez, M J
  • Animal Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, 14071 Córdoba, Spain.
Carrasco, J J
  • Equine Reproduction Center, Centro de Selección y Reproducción Animal, (CENSYRA-Extremadura Government), 06007 Badajoz, Spain.
Gómez-Arrones, V
  • Equine Reproduction Center, Centro de Selección y Reproducción Animal, (CENSYRA-Extremadura Government), 06007 Badajoz, Spain.
Calero-Carretero, R
  • Equine Reproduction Center, Centro de Selección y Reproducción Animal, (CENSYRA-Extremadura Government), 06007 Badajoz, Spain.
Hidalgo, M
  • Animal Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, 14071 Córdoba, Spain.

MeSH Terms

  • Amino Acids / chemistry
  • Amino Acids / pharmacology
  • Animals
  • Equidae / physiology
  • Male
  • Semen Analysis / veterinary
  • Semen Preservation / veterinary
  • Sperm Motility
  • Spermatozoa / drug effects
  • Spermatozoa / physiology

Citations

This article has been cited 11 times.
  1. Baqerkhani M, Soleimanzadeh A, Mohammadi R. Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology. BMC Vet Res 2024 Mar 11;20(1):99.
    doi: 10.1186/s12917-024-03915-1pubmed: 38468237google scholar: lookup
  2. Pribilova M, Skalickova S, Urbankova L, Baholet D, Nevrkla P, Kopec T, Slama P, Horky P. Monitoring of taurine dietary supplementation effect on parameters of Duroc boar ejaculate in summer season. PLoS One 2024;19(1):e0288317.
    doi: 10.1371/journal.pone.0288317pubmed: 38271350google scholar: lookup
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    doi: 10.5713/ab.22.0325pubmed: 36397685google scholar: lookup
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    doi: 10.3389/fvets.2022.1011899pubmed: 36225802google scholar: lookup
  6. Yousef MS, Megahed GA, Abozed GF, Hayder M, Abd-Elhafeez HH, Rawy MS. Exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks. Sci Rep 2022 May 12;12(1):7869.
    doi: 10.1038/s41598-022-11884-4pubmed: 35551262google scholar: lookup
  7. Zhang L, Wang Y, Sohail T, Kang Y, Niu H, Sun X, Ji D, Li Y. Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu Sheep. Animals (Basel) 2021 Sep 18;11(9).
    doi: 10.3390/ani11092725pubmed: 34573691google scholar: lookup
  8. Silvestre MA, Yániz JL, Peña FJ, Santolaria P, Castelló-Ruiz M. Role of Antioxidants in Cooled Liquid Storage of Mammal Spermatozoa. Antioxidants (Basel) 2021 Jul 8;10(7).
    doi: 10.3390/antiox10071096pubmed: 34356329google scholar: lookup
  9. Martins-Bessa A, Quaresma M, Leiva B, Calado A, Navas González FJ. Bayesian Linear Regression Modelling for Sperm Quality Parameters Using Age, Body Weight, Testicular Morphometry, and Combined Biometric Indices in Donkeys. Animals (Basel) 2021 Jan 13;11(1).
    doi: 10.3390/ani11010176pubmed: 33451023google scholar: lookup
  10. Feng C, Zhu Z, Bai W, Li R, Zheng Y, Tian X, Wu D, Lu H, Wang Y, Zeng W. Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine. Animals (Basel) 2020 Sep 1;10(9).
    doi: 10.3390/ani10091549pubmed: 32883027google scholar: lookup
  11. Dawra V, Yadav B, Yadav S. Effect of glutamine supplementation and replacement of tris-egg yolk based extender with defatted cow milk on spermatozoa quality after equilibration and thawing. Vet World 2015 Aug;8(8):1027-31.
    doi: 10.14202/vetworld.2015.1027-1031pubmed: 27047193google scholar: lookup
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