Effect of growth factors on the migration of equine oral and limb fibroblasts using an in vitro scratch assay.
Abstract: The objective of this study was to determine the effect of platelet derived growth factor BB (PDGF), epidermal growth factor (EGF), transforming growth factor β1 (TGFβ1), insulin like growth factor-1 (IGF-1) and fibroblast growth factor-2 (FGF-2) on the proliferation and migration of equine oral mucosa and leg skin fibroblast cell lines, using an in vitro scratch assay. Fibroblasts from the two sites were firstly grown to confluence and then an area of cells removed (cell void area). Cell migration alone (with the addition of the mitosis inhibitor mitomycin-C to the culture media) and proliferation and migration combined (without mitomycin-C) into the cell void area were observed at 0, 5, 10, 24 and 36 h. The presence of mitomycin-C in the culture media significantly slowed the closure of the cell void area, as mitosis was inhibited. For the oral cells only, TGFβ1 significantly slowed both migration (with mitomycin-C) and proliferation and migration combined (without mitomycin-C). For the limb cells only, both PDGF and FGF-2 significantly increased fibroblast proliferation and migration combined (without mitomycin-C). For both cell types, EGF significantly reduced migration (with mitomycin-C). IGF-1 had no effect on any of the parameters measured. It was concluded that TGFβ1, PDGF and FGF-2 have differential effects on the proliferation and migration of equine oral and limb fibroblasts. These differences in fibroblast responses to growth factors may in part form the basis of the different clinical outcomes for oral and limb wounds.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication Date: 2012-02-10 PubMed ID: 22326783DOI: 10.1016/j.tvjl.2012.01.010Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The researchers conducted a study to understand how different growth factors impact the proliferation and migration of horse oral and limb fibroblasts. The growth factors – PDGF, EGF, TGFβ1, IGF-1 and FGF-2 were tested under conditions of cell growth and movement, revealing distinct responses that could influence healing outcomes in oral and limb wounds.
Study Approach
- The team of researchers sourced fibroblast cell lines from the oral mucosa (mouth lining) and leg skin of horses. Fibroblasts are a type of cell involved in wound healing and collagen production.
- The cells were grown until they reached confluence (full coverage), after which a section of the cells was removed to create a void. This cell void allows for the observation of cell migration and proliferation into the open space.
- The researchers observed both proliferation (cell growth) and migration (movement of cells) into the void at timed intervals: 0, 5, 10, 24 and 36 hours. They tested under two conditions: strictly migration (with mitomycin-C, a mitosis inhibitor, added to the culture media) and combined proliferation and migration (without mitomycin-C).
Key Findings
- Adding mitomycin-C to the culture media notably slowed the closure of the cell void, due to the inhibition of cell division.
- The effect of individual growth factors differed between the oral and limb cells. Specifically, TGFβ1 significantly reduced both migration and combined proliferation and migration in oral cells. For the limb cells, PDGF and FGF-2 considerably enhanced combined proliferation and migration.
- EGF substantially decreased migration in both cell types while IGF-1 did not noticeably impact any of the parameters measured.
Conclusion
- The study concluded that different growth factors TGFβ1, PDGF, and FGF-2 selectively impact the growth and movement of equine oral and limb fibroblasts. They speculate that these varying fibroblast responses could contribute to the dissimilar clinical outcomes witnessed in oral and limb wounds.
- Therefore, understanding the impact of these growth factors could potentially lead to new therapies to enhance wound healing in various tissues.
Cite This Article
APA
Rose MT.
(2012).
Effect of growth factors on the migration of equine oral and limb fibroblasts using an in vitro scratch assay.
Vet J, 193(2), 539-544.
https://doi.org/10.1016/j.tvjl.2012.01.010 Publication
Researcher Affiliations
- Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Ceredigion SY23 3DA, UK. mir@aber.ac.uk
MeSH Terms
- Animals
- Area Under Curve
- Biological Assay / veterinary
- Cell Movement / drug effects
- Cell Proliferation / drug effects
- Cells, Cultured
- Dermis / cytology
- Extremities / physiology
- Fibroblasts / drug effects
- Fibroblasts / metabolism
- Horses / physiology
- Intercellular Signaling Peptides and Proteins / pharmacology
- Mitomycin / pharmacology
- Mouth Mucosa / cytology
- Nucleic Acid Synthesis Inhibitors / pharmacology
- Signal Transduction
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