Analyze Diet
Journal of equine veterinary science2021; 101; 103452; doi: 10.1016/j.jevs.2021.103452

Effect of Hay Soaking Duration on Metabolizable Energy, Total and Prececal Digestible Crude Protein and Amino Acids, Non-Starch Carbohydrates, Macronutrients and Trace Elements.

Abstract: Soaking hay before feeding has been documented to reduce airborn respirable particles and water-soluble carbohydrate (WSC) content which may have positive benefits for horses suffering from Equine Asthma (EA) or Equine Metabolic Syndrome (EMS). Prolonged soaking also leaches minerals, but to-date no measurement of the loss of small intestine digestible crude protein has been documented. One aim of this study was to investigate various soaking durations on nutrient contents of hay, WSC, macronutrients, and trace elements levels. Another objective was to determine the prececal digestible crude protein (pcd CP) and amino acid (pcd AA) fraction contents and prececal digestibility (pcD). Four different batches of meadow hay were soaked in water (20° C during preparation) for 0 minutes, 15 minutes, 30 minutes, 60 minutes, and 12 hours and drained for 20 minutes. A 15 minutes soaking duration significantly reduced the levels of nearly all investigated nutrients (e.g. for fructans, and WSC, macronutrients and trace elements). However, the crude fiber, acid detergent fiber (ADF), and acid detergent lignin content increased. Metabolizable energy (ME) contents decreased by 5-15 %, pcd CP and pcd AA fell by 35 %, and the pcD declined by up to 49 %. In contrast, the pcD was 56 % before soaking. Longer soaking durations did not enhance the wash-out effect. Horse owners should be aware that soaking hay, regardless of reason, may negatively alter the nutritional value. The wide range of wash-out effects may pose risks in calculating the correct dry matter portion to prevent weight loss and maintain ME, pcd CP and pcd AA requirements, especially for horses with EMS and EA.
Publication Date: 2021-03-26 PubMed ID: 33993927DOI: 10.1016/j.jevs.2021.103452Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates how the length of time hay is soaked in water impacts its nutritional value. The results indicate overall nutrient reduction since essential elements including crude proteins, amino acids, carbohydrates, and others are washed away, which could negatively affect horse health, particularly those suffering from Equine Asthma (EA) or Equine Metabolic Syndrome (EMS).

Research Purpose

  • The research aimed to understand the effect of different soaking durations on the nutrient content of hay, including water-soluble carbohydrates (WSC), macronutrients, and trace elements.
  • The study also sought to measure the prececal digestible crude protein and amino acid content, as well as prececal digestibility rates across various soaking times.

Methodology

  • Four batches of meadow hay were soaked in water at a temperature of 20°C for 0 minutes, 15 minutes, 30 minutes, 60 minutes, and 12 hours, and subsequently drained for 20 minutes.
  • The researchers then measured for various nutritional elements including metabolizable energy, digestible crude protein, amino acids, WSC, macronutrients, and trace elements.

Research Findings

  • The research found that a soaking duration of 15 minutes significantly reduced the levels of nearly all investigated nutrients, including fructans, WSC, macronutrients, and trace elements.
  • The metabolizable energy content decreased by 5-15%, digestible crude protein and amino acid volume fell by 35%, and prececal digestibility declined by up to 49%.
  • However, different elements like crude fiber, acid detergent fiber, and acid detergent lignin content were observed to increase.
  • This study also found that longer soaking durations did not enhance the wash-out effect, meaning these nutrients were not further depleted with additional soaking time.

Implications and Conclusions

  • These findings imply that horse owners must be aware of the possibility that soaking hay, regardless of the reason, may decrease its nutritional value. Thus, it could potentially harm the health of the horse, especially for individuals with specific health conditions like EA or EMS.
  • Due to the wide-ranging effects, correctly calculating the right measure of dry matter to prevent weight loss, maintain required metabolizable energy, and fulfill protein and amino acid requirements could become challenging.

Cite This Article

APA
Bochnia M, Pietsch C, Wensch-Dorendorf M, Greef M, Zeyner A. (2021). Effect of Hay Soaking Duration on Metabolizable Energy, Total and Prececal Digestible Crude Protein and Amino Acids, Non-Starch Carbohydrates, Macronutrients and Trace Elements. J Equine Vet Sci, 101, 103452. https://doi.org/10.1016/j.jevs.2021.103452

Publication

ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Volume: 101
Pages: 103452
PII: S0737-0806(21)00082-4

Researcher Affiliations

Bochnia, M
  • Martin Luther University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Group Animal Nutrition, 06112 Halle (Saale), Germany.
Pietsch, C
  • Martin Luther University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Group Animal Nutrition, 06112 Halle (Saale), Germany.
Wensch-Dorendorf, M
  • Martin Luther University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Biometrics and Informatics in Agriculture Group, 06112 Halle (Saale), Germany.
Greef, M
  • Julius Kuehn Institute, Federal Research Center for Cultivated Plants, Crop and Soil Science, 38116 Braunschweig, Germany.
Zeyner, A
  • Martin Luther University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Group Animal Nutrition, 06112 Halle (Saale), Germany. Electronic address: annette.zeyner@landw.uni-halle.de.

MeSH Terms

  • Amino Acids
  • Animal Feed / analysis
  • Animals
  • Carbohydrates
  • Horses
  • Nutrients
  • Trace Elements

Citations

This article has been cited 4 times.
  1. Pisch C, Wensch-Dorendorf M, Schwarzenbolz U, Henle T, Greef JM, Zeyner A. Effect of Hay Steaming on the Estimated Precaecal Digestibility of Crude Protein and Selected Amino Acids in Horses. Animals (Basel) 2022 Nov 10;12(22).
    doi: 10.3390/ani12223092pubmed: 36428320google scholar: lookup
  2. Glatter M, Bochnia M, Wensch-Dorendorf M, Greef JM, Zeyner A. Feed Intake Parameters of Horses Fed Soaked or Steamed Hay and Hygienic Quality of Hay Stored following Treatment. Animals (Basel) 2021 Sep 18;11(9).
    doi: 10.3390/ani11092729pubmed: 34573695google scholar: lookup
  3. Maier I, Kienzle E. A Meta-Analysis on Quantitative Sodium, Potassium and Chloride Metabolism in Horses and Ponies. Animals (Basel) 2025 Jan 13;15(2).
    doi: 10.3390/ani15020191pubmed: 39858191google scholar: lookup
  4. Diez de Castro E, Fernandez-Molina JM. Environmental Management of Equine Asthma. Animals (Basel) 2024 Jan 30;14(3).
    doi: 10.3390/ani14030446pubmed: 38338089google scholar: lookup