Effect of homologous preovulatory follicular fluid on in vitro maturation of equine cumulus-oocyte complexes.
Abstract: The objective of this study was to test the hypothesis that incubating equine cumulus-oocyte complexes (COCs) in medium containing 50% or 100% homologous preovulatory follicular fluid would improve cumulus expansion and nuclear maturation. Oocytes were incubated in one of three media: 1) supplemented TCM-199 (control), 2) 50% (v/v) follicular fluid in control medium or 3) 100% follicular fluid. Cumulus expansion was evaluated subjectively, and nuclear maturation was evaluated by staining oocytes with Hoechst 33258. The hypothesis that incubating COCs in medium containing follicular fluid would improve cumulus expansion was supported. More (P < 0.05) compact COCs incubated in 50% or 100% follicular fluid developed a moderately to completely expanded cumulus after 24 and 36 h of incubation and more (P < 0.05) expanded COCs incubated in 100% follicular fluid developed a moderately to completely expanded cumulus after 36 h of incubation compared to control medium. The hypothesis that incubating COCs in medium containing follicular fluid would improve nuclear maturation was not supported. Although more (P < 0.05) compact COCs incubated in 50% follicular fluid reached polar body-stage compared to those in control medium, the nuclear maturation rate in the control medium was lower than it was when the same medium was used in a preliminary experiment (described in main text); therefore, the apparent superiority of 50% follicular fluid must be interpreted cautiously. Based on these results, future studies are warranted to further address the value of adding preovulatory follicular fluid to equine IVM culture systems.
Publication Date: 2001-10-23 PubMed ID: 11665878DOI: 10.1016/s0093-691x(01)00604-5Google Scholar: Lookup
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- Journal Article
Summary
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The study aims to investigate whether incubating horse egg cells (cumulus-oocyte complexes) in fluid from the follicle prior to ovulation would improve cell expansion and maturation. Their findings suggest that the use of follicular fluid does indeed aid cell expansion, but does not conclusively promote cell maturation.
Objective of the Study
- The primary objective of this research was to assess the impact of incubating equine cumulus-oocyte complexes (COCs) in a medium containing 50% or 100% homologous preovulatory follicular fluid. The researchers endeavored to determine if this process would enhance the cumulus expansion (the growth and development of the cells that surround the oocyte) and nuclear maturation (the progress of the oocyte’s nucleus to a mature, ready-for-fertilization stage).
Methodology
- The researchers incubated the oocytes in three different types of media: standard TCM-199 (serving as a control), a medium with 50% follicular fluid, and a medium with 100% follicular fluid.
- Cumulus expansion was subjectively judged, and nuclear maturation was evaluated by staining the oocytes with a compound called Hoechst 33258, commonly used in cell biology to stain DNA.
Findings
- The hypothesis that cumulus expansion would be improved by incubating COCs in a medium containing follicular fluid was supported by the study.
- Significantly more COCs incubated in 50% or 100% follicular fluid displayed moderate to complete cumulus expansion after 24 and 36 hours of incubation compared to those in the control medium.
- Contrary to expectations, the study found no decisive evidence that nuclear maturation was enhanced by incubating the COCs in follicular fluid. Even though more COCs incubated in 50% follicular fluid reached the polar body-stage, this finding must be interpreted with caution, as the control medium had a lower nuclear maturation rate than usual.
Conclusion
- Based on the obtained outcomes, the authors suggest that additional research is necessary to further investigate the potential benefits of applying preovulatory follicular fluid to the equine in vitro maturation (IVM) culture systems, more specifically, in relation to improving nuclear maturation.
Cite This Article
APA
Aguilar JJ, Woods GL, Miragaya MH, Olsen LM, Vanderwall DK.
(2001).
Effect of homologous preovulatory follicular fluid on in vitro maturation of equine cumulus-oocyte complexes.
Theriogenology, 56(5), 745-758.
https://doi.org/10.1016/s0093-691x(01)00604-5 Publication
Researcher Affiliations
- Department of Animal and Veterinary Science, University of Idaho, Moscow 83844-2201 USA.
MeSH Terms
- Animals
- Bisbenzimidazole
- Cell Nucleus / physiology
- Cells, Cultured
- Culture Media
- Female
- Follicular Fluid / physiology
- Horses / physiology
- Oocytes / physiology
- Oocytes / ultrastructure
- Ovarian Follicle / cytology
- Ovulation
- Time Factors
Citations
This article has been cited 2 times.- Hasegawa J, Yanaihara A, Iwasaki S, Mitsukawa K, Negishi M, Okai T. Reduction of connexin 43 in human cumulus cells yields good embryo competence during ICSI. J Assist Reprod Genet 2007 Oct;24(10):463-6.
- Dell'Aquila ME, Caillaud M, Maritato F, Martoriati A, Gérard N, Aiudi G, Minoia P, Goudet G. Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis. Reprod Biol Endocrinol 2004 Jun 22;2:44.
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