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Home / Equine Research Bank / J Biomed Mater Res A / Effect of hyaluronic acid amide derivative on equine synovia...
Journal of biomedical materials research. Part A2009; 92(3); 1162-1170; doi: 10.1002/jbm.a.32455

Effect of hyaluronic acid amide derivative on equine synovial fluid viscoelasticity.

Abstract: An amphiphilic hyaluronic acid (HA) derivative has been obtained by the amidation of the carboxylic group of the glucuronic acid. This derivative, HYADD4-G (HY4), is the hexadecylamide of 500-730 kDa hyaluronic acid, derived from Streptococcus equi at about 2% degree of substitution (2 mol hexadecylamine per 100 mol hexuronic acid). Its viscoelastic properties, at a concentration of 5 mg/mL in phosphate buffer saline, have been compared with those solutions of native HA, having the same molecular weight. Changes in the viscoelastic properties of equine synovial fluid (SF) when mixed with HY4 over a series of volume ratios-viz 1:2, 1:1, 3:1, and 7:1-have been evaluated. HY4 is able to associate into aqueous solution, and its rheological behavior is typical of a weak gel. Throughout the frequency range investigated (0.1-10 Hz), the elastic modulus G' is higher than the viscous modulus G'', and both moduli are frequency independent, and G' value is about two orders of magnitude higher than that of a comparable solution of native HA. The addition of HY4 to equine synovial fluid (SF) increased its viscoelasticity at all the SF:HY4 ratios tested. These results demonstrate that HY4 is able to integrate with SF, increasing the synovial fluid rheology, and could be an interesting new option in viscosupplement therapy of osteoarthritis, particularly considering its low degree of chemical modification from native HA.
(c) 2009 Wiley Periodicals, Inc.
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Publication Date: 2009-03-27 PubMed ID: 19322881DOI: 10.1002/jbm.a.32455Google Scholar: Lookup
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  • Journal Article

Summary

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The research article explores the impact of a hyaluronic acid derivative, known as HY4, on the viscoelastic properties of horse synovial fluid. The researchers found that the addition of HY4 increases the fluid’s viscoelasticity, suggesting potential uses in treating osteoarthritis.

Introduction and Objective

  • The key objective of the study was to investigate the impact of a hyaluronic acid (HA) derivative on the viscoelastic properties of equine (horse) synovial fluid. The derivative, known as HY4, was derived from Streptococcus equi, a bacterium common in horses.
  • The underlying motivation for this research is that improvements in the viscoelastic properties of synovial fluid could potentially lead to effective treatments for osteoarthritis, a common joint disorder.

Hyaluronic Acid Derivative

  • Hyaluronic acid (HA) is a common component of synovial fluid, which lubricates and cushions joints. The research focuses on a derived version of HA, known as HY4. HY4 has been made amphiphilic (both water and fat soluble) through the process of amidation, which essentially changes its properties and behavior in solutions.
  • The concentration of HY4 used in the research was 5mg/mL in a phosphate buffer saline solution, similar in terms of molecular weight to native HA.

Experimental Process and Findings

  • Different volume ratios of HY4 and synovial fluid were mixed and their viscoelastic properties were evaluated. As the frequency range of 0.1-10 Hz was examined, researchers noted that the elastic modulus G’ was higher than the viscous modulus G”, and both moduli were found to be frequency independent.
  • In comparison to native HA, the G’ value of the HY4 solution was found to be two orders of magnitude higher. This indicates a higher resistance to deformation.
  • At all the tested volume ratios, the addition of HY4 increased the viscoelasticity of the synovial fluid. Thus, it was observed that HY4 is capable of integrating with the synovial fluid to improve its rheology.

Implications

  • The increased viscoelasticity of the synovial fluid by HY4 could potentially be beneficial in the treatment of osteoarthritis. By improving the cushioning and lubricating properties of synovial fluid, HY4 could provide relief from the painful symptoms of the disease.
  • Another significant aspect of this research is the low degree of chemical modification from native HA to HY4. This suggests that the treatment may have fewer side effects compared to other viscosupplements.

Cite This Article

APA
Borzacchiello A, Mayol L, Schiavinato A, Ambrosio L. (2009). Effect of hyaluronic acid amide derivative on equine synovial fluid viscoelasticity. J Biomed Mater Res A, 92(3), 1162-1170. https://doi.org/10.1002/jbm.a.32455

Publication

Journal of biomedical materials research. Part A
ISSN: 1552-4965
NlmUniqueID: 101234237
Country: United States
Language: English
Volume: 92
Issue: 3
Pages: 1162-1170

Researcher Affiliations

Borzacchiello, A
  • Institute of Composite and Biomedical Materials, CNR and CRIB, University of Naples, Federico II, Naples, Italy. bassunta@unina.it
Mayol, L
    Schiavinato, A
      Ambrosio, L

        MeSH Terms

        • Amides / chemistry
        • Animals
        • Horses
        • Hyaluronic Acid / administration & dosage
        • Hyaluronic Acid / chemistry
        • Synovial Fluid / physiology
        • Viscosity

        Citations

        This article has been cited 17 times.
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          doi: 10.3390/gels9100801pubmed: 37888374google scholar: lookup
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