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Home / Equine Research Bank / Multidiscip Respir Med / Effect of inhaled hydrosoluble curcumin on inflammatory mark...
Multidisciplinary respiratory medicine2015; 10(1); 16; doi: 10.1186/s40248-015-0010-7

Effect of inhaled hydrosoluble curcumin on inflammatory markers in broncho-alveolar lavage fluid of horses with LPS-induced lung neutrophilia.

Abstract: Horses commonly suffer from chronic respiratory disease and are also used in large animal models of spontaneous or induced airway inflammation. The anti-inflammatory properties of curcumin are largely described but its low bioavailability precludes its clinical use. NDS27, a lysin salt of curcumin incorporated in beta-cyclodextrine, has high bioavailability and can be administered by inhalation. The aim of this study was to investigate the effects of inhaled NDS27 on inflammatory cytokines and proteins in the broncho-alveolar lavage fluid using a model of neutrophilic airway inflammation. Methods: Airway neutrophilia was induced in eight horses by inhalation of lipopolysaccharides (LPS). Horses were treated with either inhalation of NDS27 or with placebo in a randomized cross-over design. Broncho-alveolar lavages were performed 6 hours after stimulation with LPS. Percentage of neutrophils, concentrations of IL-1β, TNF-α, IL-6, Club cell secretory protein, myeloperoxidase (MPO) and elastase (ELT) concentrations were determined. Results: LPS stimulation induced significant increases in neutrophil counts and concentrations of IL-6 (70.2 ± 66.0 pg/ml), TNF-α (43.9 ± 31.2 pg/ml), MPO (580.9 ± 327.0 ng/ml) and ELT (27.6 ± 16.7 ng/ml). Treatment with NDS27 significantly prevented the increase in active and total MPO (216.4 ± 118.1 ng/ml) and ELT (5.9 ± 3.2 ng/ml) while there was a trend towards reduced IL-6 concentration. Conclusions: Results show that, although not reducing neutrophil recruitment, NDS27 largely abolishes LPS-induced neutrophil degranulation. Reduced levels of ELT and MPO, as well as reduced MPO activity may have beneficial effects via a reduced production of reactive oxygen species implicated in chronic inflammation and airway remodeling.
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Publication Date: 2015-04-15 PubMed ID: 25908974PubMed Central: PMC4407840DOI: 10.1186/s40248-015-0010-7Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored how inhaling NDS27, a highly bioavailable form of curcumin, effects horses suffering from respiratory disease. The study found that while NDS27 doesn’t reduce the recruitment of neutrophils, it does suppress their degranulation, leading to a potential reduction in chronic inflammation and airway remodeling.

Study Introduction and Purpose

  • The study is based on the context that horses often experience chronic respiratory diseases and are also used in large animal models for investigation of spontaneous or induced airway inflammation.
  • Curcumin, a known anti-inflammatory compound, is often not used clinically because of its low bioavailability.
  • NDS27, a variation of curcumin incorporated in beta-cyclodextrine, has been identified as having higher bioavailability and can be administered by inhalation.
  • The study aimed to examine the effects of inhaled NDS27 on inflammatory cytokines and proteins in broncho-alveolar lavage fluid in a model of neutrophilic airway inflammation.

Methodology

  • Airway neutrophilia, which is a condition characterized by an abnormal increase in neutrophils, was induced in eight horses through the inhalation of lipopolysaccharides (LPS).
  • The horses were treated either with an inhalation of NDS27 or a placebo, following a randomized cross-over design.
  • Broncho-alveolar lavages were then performed 6 hours after being stimulated with LPS.
  • The study determined the percentage of neutrophils and concentrations of various inflammation markers.

Results

  • LPS stimulation caused significant increases in neutrophil counts and concentrations of various inflammation markers, indicative of inflammation and neutrophil activity.
  • The treatment with NDS27 led to a significant prevention in the increase of active and total MPO and ELT.
  • There was a trend observed towards reduced IL-6 concentration after administering NDS27.

Conclusions

  • The study concluded that NDS27, though unable to reduce neutrophil recruitment, was able to greatly limit LPS-induced neutrophil degranulation.
  • Reduced levels of ELT and MPO, as well as reduced MPO activity could potentially have beneficial effects through lowering the production of reactive oxygen species. These oxygen species are implicated in chronic inflammation and airway remodeling.

Cite This Article

APA
Sandersen C, Bienzle D, Cerri S, Franck T, Derochette S, Neven P, Mouytis-Mickalad A, Serteyn D. (2015). Effect of inhaled hydrosoluble curcumin on inflammatory markers in broncho-alveolar lavage fluid of horses with LPS-induced lung neutrophilia. Multidiscip Respir Med, 10(1), 16. https://doi.org/10.1186/s40248-015-0010-7

Publication

Multidisciplinary respiratory medicine
ISSN: 1828-695X
NlmUniqueID: 101477642
Country: Italy
Language: English
Volume: 10
Issue: 1
Pages: 16
PII: 16

Researcher Affiliations

Sandersen, Charlotte
  • Equine Clinic, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
Bienzle, Dorothee
  • Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1 Canada.
Cerri, Simona
  • Equine Clinic, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
Franck, Thierry
  • Equine Clinic, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
Derochette, Sandrine
  • Center for Oxygen Research and Development, University of Liege, Sart Tilman, B6a, 4000 Belgium.
Neven, Philippe
  • Center for Oxygen Research and Development, University of Liege, Sart Tilman, B6a, 4000 Belgium.
Mouytis-Mickalad, Ange
  • Center for Oxygen Research and Development, University of Liege, Sart Tilman, B6a, 4000 Belgium.
Serteyn, Didier
  • Equine Clinic, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium ; Center for Oxygen Research and Development, University of Liege, Sart Tilman, B6a, 4000 Belgium.

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Citations

This article has been cited 3 times.
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