Effect of initial cumulus morphology on meiotic dynamic and status of mitochondria in horse oocytes during IVM.
Abstract: The aim of this investigation was to examine the chromatin configuration of the nucleus, pattern of mitochondrial aggregation and mitochondrial activity in parallel studies in the same horse oocytes. Horse oocytes recovered by ultrasound-guided follicle aspiration in vivo were classified according to two main initial cumulus morphologies as having compact or expanded cumulus. The percentage of oocytes with a diplotene meiotic configuration at the time of recovery from the follicles was highest in compact oocytes. Oocytes with expanded cumulus layers at the time of recovery matured more rapidly in vitro and reached a proportion >50% at the metaphase II stage (M 2) sooner during in vitro maturation (IVM), than did compact oocytes. The mitochondrial aggregation pattern changed from finely distributed (Type 1) through crystalline (Type 2) to an aggregated, granulated appearance (Type 3) during IVM. The pattern of mitochondrial aggregation at the time of recovery was associated with the initial cumulus morphology of the oocyte, in that compact oocytes had a higher proportion of Type 1 aggregation, whereas expanded oocytes had a higher proportion of Type 3. The fluorescence intensity of metabolic active mitochondria, measured by fluorescence intensity (Em 570) per oocyte after MitoTracker CMTM Ros orange labelling, increased in the oocytes during IVM and depended on initial cumulus investment. Oocytes with the granulated type of aggregated mitochondria Type 3 had the highest level of metabolic activity and were in more progressed stages of meiosis (A 1-M 2). Oocytes initially having expanded layers of cumulus reached significantly higher levels of mitochondrial activity after IVM than did oocytes initially having compact cumuli. During resumption of meiosis the mitochondrial activity of oocytes with initially expanded cumulus increased continuously up to M 2, whereas in oocytes from compact cumulus-oocyte complex (COC), the activity declined after A 1/T 1 stages of meiosis.
Publication Date: 2007-03-14 PubMed ID: 17348975DOI: 10.1111/j.1439-0531.2006.00749.xGoogle Scholar: Lookup
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Summary
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This research investigates the impact of the initial form of cumulus cells, either compact or expanded, on the meiotic dynamics and mitochondrial activity of horse oocytes during in-vitro maturation. The results revealed a correlation between cumulus morphology and mitochondria activity, a key marker of successful oocyte maturation.
Objective and Method
- The study’s main objective was to determine the effect of different cumulus morphologies – compact and expanded – on the meiotic progression and mitochondrial activity in horse oocytes during in-vitro maturation.
- Horse oocytes were collected through in-vivo ultrasound-guided follicle aspiration and classified based on their cumulus morphology.
Key Findings – Compact Oocytes
- Compact horse oocytes had a higher proportion in a diplotene meiotic configuration at the time of recovery from the follicles, indicating these cells were in an earlier stage of meiosis.
- Compact oocyte’s rate of maturation was slower in vitro compared to oocytes with an expanded cumulus.
- With regards to mitochondrial distribution, compact oocytes predominantly showed a more finely distributed pattern (Type 1).
- After in-vitro maturation, compact oocytes showed a decline in mitochondrial activity after reaching certain stages of meiosis.
Key Findings – Expanded Oocytes
- Oocytes with expanded cumulus layers matured more rapidly in vitro and reached the intended stage (> 50% at metaphase II stage) faster during in-vitro maturation compared to compact oocytes.
- Expanded oocytes displayed a higher proportion of a more aggregated, granulated mitochondrial pattern (Type 3).
- Compared to compact oocytes, the expanded oocytes achieved higher levels of mitochondrial activity during the in-vitro maturation process. Mitochondrial activity continuously increased up to the metaphase II stage.
- In terms of meiotic progression, oocytes initially having expanded cumulus reached more advanced stages (A 1 – M 2).
Implications and Conclusion
- The cumulus morphology of horse oocytes had a significant impact on key factors of oocyte maturation – meiotic progression and mitochondrial activity.
- Oocytes with expanded cumulus morphology showed a better rate and extent of maturation in vitro compared to compact oocytes, as evident from the mitochondrial configurations and activities observed.
- The findings highlight the value of considering the initial cumulus morphology in in-vitro oocyte maturation approaches – an expanded morphology indicating a potentially more successful maturation process.
Cite This Article
APA
Torner H, Alm H, Kanitz W, Goellnitz K, Becker F, Poehland R, Bruessow KP, Tuchscherer A.
(2007).
Effect of initial cumulus morphology on meiotic dynamic and status of mitochondria in horse oocytes during IVM.
Reprod Domest Anim, 42(2), 176-183.
https://doi.org/10.1111/j.1439-0531.2006.00749.x Publication
Researcher Affiliations
- Department of Reproductive Biology, Research Institute for the Biology of Farm Animals, Dunnerstorf, Germany. torner@fbn-dummerstorf.de
MeSH Terms
- Animals
- Cells, Cultured
- Chromatin / ultrastructure
- Female
- Horses / physiology
- Meiosis / physiology
- Mitochondria / metabolism
- Mitochondria / ultrastructure
- Oocytes / physiology
- Ovarian Follicle / cytology
- Staining and Labeling / veterinary
Citations
This article has been cited 14 times.- Ashraf R, Rashid S, Rasheed I, Asif S. Early embryonic death in equines and camelids.. Open Vet J 2022 Nov-Dec;12(6):903-909.
- Felix MR, Turner RM, Dobbie T, Hinrichs K. Successful in vitro fertilization in the horse: production of blastocysts and birth of foals after prolonged sperm incubation for capacitation†.. Biol Reprod 2022 Dec 10;107(6):1551-1564.
- Pollard CL, Younan A, Swegen A, Gibb Z, Grupen CG. Insights into the NAD(+) biosynthesis pathways involved during meiotic maturation and spindle formation in porcine oocytes.. J Reprod Dev 2022 Jun 1;68(3):216-224.
- Kirillova A, Smitz JEJ, Sukhikh GT, Mazunin I. The Role of Mitochondria in Oocyte Maturation.. Cells 2021 Sep 19;10(9).
- Lodde V, Luciano AM, Musmeci G, Miclea I, Tessaro I, Aru M, Albertini DF, Franciosi F. A Nuclear and Cytoplasmic Characterization of Bovine Oocytes Reveals That Cysteamine Partially Rescues the Embryo Development in a Model of Low Ovarian Reserve.. Animals (Basel) 2021 Jun 29;11(7).
- de Andrade Melo-Sterza F, Poehland R. Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions.. Int J Mol Sci 2021 Mar 26;22(7).
- Piras AR, Menéndez-Blanco I, Soto-Heras S, Catalá MG, Izquierdo D, Bogliolo L, Paramio MT. Resveratrol supplementation during in vitro maturation improves embryo development of prepubertal goat oocytes selected by brilliant cresyl blue staining.. J Reprod Dev 2019 Apr 12;65(2):113-120.
- Lee W, Song K, Lee I, Shin H, Lee BC, Yeon S, Jang G. Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system.. J Vet Sci 2015;16(4):509-16.
- Leoni GG, Palmerini MG, Satta V, Succu S, Pasciu V, Zinellu A, Carru C, Macchiarelli G, Nottola SA, Naitana S, Berlinguer F. Differences in the Kinetic of the First Meiotic Division and in Active Mitochondrial Distribution between Prepubertal and Adult Oocytes Mirror Differences in their Developmental Competence in a Sheep Model.. PLoS One 2015;10(4):e0124911.
- Martino NA, Dell'Aquila ME, Filioli Uranio M, Rutigliano L, Nicassio M, Lacalandra GM, Hinrichs K. Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential.. Reprod Biol Endocrinol 2014 Oct 11;12:99.
- Abdoon AS, Abdel-Rahman HA, Shawki SM, Kandil OM, Fathalla SI. Influence of follicle size, methods of retrieval on oocytes yield and morphology in Egyptian Jennies ovaries with special reference to maturation rate in vitro.. Vet Res Commun 2014 Dec;38(4):287-95.
- Dell'Aquila ME, Bogliolo L, Russo R, Martino NA, Filioli Uranio M, Ariu F, Amati F, Sardanelli AM, Linsalata V, Ferruzzi MG, Cardinali A, Minervini F. Prooxidant effects of verbascoside, a bioactive compound from olive oil mill wastewater, on in vitro developmental potential of ovine prepubertal oocytes and bioenergetic/oxidative stress parameters of fresh and vitrified oocytes.. Biomed Res Int 2014;2014:878062.
- Russo R, Monaco D, Rubessa M, El-Bahrawy KA, El-Sayed A, Martino NA, Beneult B, Ciannarella F, Dell'Aquila ME, Lacalandra GM, Filioli Uranio M. Confocal fluorescence assessment of bioenergy/redox status of dromedary camel (Camelus dromedarius) oocytes before and after in vitro maturation.. Reprod Biol Endocrinol 2014 Feb 18;12:16.
- Lei T, Guo N, Tan MH, Li YF. Effect of mouse oocyte vitrification on mitochondrial membrane potential and distribution.. J Huazhong Univ Sci Technolog Med Sci 2014 Feb;34(1):99-102.
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