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Home / Equine Research Bank / Animals (Basel) / Effect of Intrauterine Infusion of Equine Fresh Platelets-Ri...
Animals : an open access journal from MDPI2021; 11(4); doi: 10.3390/ani11041123

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares.

Abstract: This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GFequina) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phase; and the third group (L-GFequina Group), consisting of mares that were intrauterine infused with 20 mL of reconstituted lyophilized horse platelets growth factors (L-GFequina) on the second day after the estrus phase. The results showed no significant difference between control and treated groups in the diameter of the preovulatory follicles during the post treatment cycle. The endometrium thickness increased significantly in the L-GFequina and PRP groups ahead of the non-treated group. Intrauterine L-GFequina or PRP administration shortened the estrus cycle length. A higher pregnancy rate was recorded in the L-GFequina and PRP treated mares. In conclusion, the intrauterine infusion of L-GFequina or PRP increased the endometrial thickness and pregnancy rate and could be used to improve fertility in Arabian purebred mares who experienced from repeat breeding.
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Publication Date: 2021-04-14 PubMed ID: 33919901PubMed Central: PMC8070911DOI: 10.3390/ani11041123Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated if the infusion of substances like platelet-rich plasma (PRP) or lyophilized growth factor (L-GF) into the uterus of Arabian mares could enhance fertility. Following the infusion, beneficial effects were observed in the thickness of the uterine lining and pregnancy rates, suggesting these treatments could potentially improve fertility in repeat breeding mares.

Objective and Methodology

  • The researchers aimed to explore the impact of injecting PRP or L-GF into the uterus of Arabian mares on follicular growth, thickness of the uterine lining, length of estrus cycle, and pregnancy rates. This was intended to formulate potential solutions for infertility in mares that had tried breeding multiple times without success.
  • A total of 73 Arabian mares who had tried breeding for three cycles without success were split randomly into three groups. The control group remained untreated, one group was administered with PRP after the estrus phase, and the third group with L-GF following the estrus phase.

Findings and Conclusions

  • Observations post the treatment cycle did not show a significant difference in the diameter of preovulatory follicles between the control and treated groups.
  • A significant increase was noted in the thickness of the uterine lining in the L-GF and PRP groups as compared with the non-treated group. The thickness of the uterine lining is a critical factor in the successful implantation of the embryo, thus influencing fertility.
  • Both L-GF and PRP administration led to a shortening of the estrus cycle. This is a positive outcome as a shorter estrus cycle is typically associated with improved fertility in mares.
  • Increased pregnancy rates were observed in the mares treated with L-GF and PRP. The positive effect of these substances on pregnancy rates suggests improvement in fertility.
  • In conclusion, the study indicated that the intrauterine infusion of L-GF or PRP could increase the thickness of the uterine lining and pregnancy rates, thereby potentially offering an effective method of enhancing fertility in Arabian mares who experience repeat unsuccessful breeding cycles.

Cite This Article

APA
Dawod A, Miro J, Elbaz HT, Fahmy H, Abdoon AS. (2021). Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares. Animals (Basel), 11(4). https://doi.org/10.3390/ani11041123

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 4

Researcher Affiliations

Dawod, Ahmed
  • Husbandry and Animal Wealth Development Department, Faculty of Veterinary Medicine, University of Sadat City, Menofia 32897, Egypt.
Miro, Jordi
  • Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, E-08193 Barcelona, Spain.
Elbaz, Hamed T
  • Theriogenology Department, Faculty of Veterinary Medicine, University of Sadat City, Menofia 32897, Egypt.
Fahmy, Hossam
  • Faculty of Medicine, Ain Shams University, Cairo 11341, Egypt.
Abdoon, Ahmed S
  • Department of Animal Reproduction and Artificial Insemination, National Research Center, Giza 12622, Egypt.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 39 references
  1. Arabian Horse Association. Horse of the Desert Bedouin. Arabian Horse History & Heritage Arabian Horse Association; Aurora, CO, USA: 2019.
  2. Canisso I.F., Segabinazzi L.G., Fedorka C.E.. Review: Persistent Breeding-Induced Endometritis in Mares—A Multifaceted Challenge: From Clinical Aspects to Immunopathogenesis and Pathobiology. Int. J. Mol. Sci. 2020;21:1432.
    doi: 10.3390/ijms21041432pmc: PMC7073041pubmed: 32093296google scholar: lookup
  3. Woodward E.M., Christoffersen M., Campos J., Squires E.L., Troedsson M.H.T.. Susceptibility to persistent breeding-induced endometritis in the mare: Relationship to endometrial biopsy score and age, and variations between seasons. Theriogenology 2012;78:495–501.
    doi: 10.1016/j.theriogenology.2012.02.028pubmed: 22494681google scholar: lookup
  4. Troedsson M.H.T.. Problems after breeding. J. Equine Vet. Sci. 2008;28:635–639.
    doi: 10.1016/j.jevs.2008.10.011google scholar: lookup
  5. Canisso I.F., Stewart J., Coutinho da Silva M.A.. Endometritis: Managing persistent post-breeding endometritis. Vet. Clin. N. Am. Equine Pract. 2016;32:465–480.
    doi: 10.1016/j.cveq.2016.08.004pubmed: 27810036google scholar: lookup
  6. Scoggin C.F.. Endometritis: Nontraditional therapies. Vet. Clin. N. Am. Equine Pract. 2016;32:499–511.
    doi: 10.1016/j.cveq.2016.08.002pubmed: 27726985google scholar: lookup
  7. Bucca S., Carli A., Buckley T., Dolci G., Fogarty U.. The use of dexamethasone administered to mares at breeding time in the modulation of persistent mating induced endometritis. Theriogenology 2008;70:1093–1100.
    doi: 10.1016/j.theriogenology.2008.06.029pubmed: 18649933google scholar: lookup
  8. Rojer H., Aurich C.. Treatment of persistent mating-induced endometritis in mares with the non-steroid anti-inflammatory drug vedaprofen. Reprod. Domest. Anim. 2010;45:458–460.
    doi: 10.1111/j.1439-0531.2009.01572.xpubmed: 20074320google scholar: lookup
  9. Brinsko S.P., Varner D.D., Blanchard T.L.. The effect of uterine lavage performed four hours post L-GFequina insemination on pregnancy rate in mares. Theriogenology 1991;35:1111–1119.
    doi: 10.1016/0093-691X(91)90358-Kgoogle scholar: lookup
  10. Roh Y.H., Kim W., Park K.U., Oh J.H.. Cytokine-release kinetics of Platelets-rich plasma according to various activation protocols. Bone Joint Res. 2016;5:37–45.
    doi: 10.1302/2046-3758.52.2000540pmc: PMC4852788pubmed: 26862077google scholar: lookup
  11. Arora S., Doda V., Kotwal U., Dogra M.. Quantification of Platelets and Platelets derived growth factors from Platelets-rich-plasma (PRP) prepared at different centrifugal force (g) and time. Transfus. Apher. Sci. 2006;54:103–110.
    doi: 10.1016/j.transci.2016.01.028pubmed: 26883885google scholar: lookup
  12. Everts P.A., Brown Mahoney C., Hoffmann J.J., Schönberger J.P., Box H.A., Van Zundert A., Knape J.T.. Platelets-rich plasma preparation using three devices: Implications for Platelets activation and Platelets growth factor release. Growth Factors 2006;24:165–171.
    doi: 10.1080/08977190600821327pubmed: 17079200google scholar: lookup
  13. Anitua E., Andia I., Ardanza B., Nurden P., Nurden A.T.. Autologous Platelets as a source of proteins for healing and tissue regeneration. Thromb. Haemost. 2004;91:4–15.
    doi: 10.1160/TH03-07-0440pubmed: 14691563google scholar: lookup
  14. Gentile P., Orlandi A., Scioli M.G., Di Pasquali C., Bocchini I., Cervelli V.. Concise review: Adipose-derived stromal vascular fraction cells and Platelets-rich plasma: Basic and clinical implications for tissue engineering therapies in regenerative surgery. Stem Cells Transl. Med. 2012;1:230–236.
    doi: 10.5966/sctm.2011-0054pmc: PMC3659840pubmed: 23197782google scholar: lookup
  15. Reghini M.F.S., Neto C.R., Segabinazzi L.G., Castro Chaves M.M.B., Dell’Aqua C.F., Bussiere M.C.C., Dell’Aqua J.A., Jr., Papa F.O., Alvarenga M.A.. Inflammatory response in chronic degenerative endometritis mares treated with Platelets-rich plasma. Theriogenology 2016;86:516–522.
    doi: 10.1016/j.theriogenology.2016.01.029pubmed: 27020400google scholar: lookup
  16. Segabinazzi L.G., Friso A.M., Correal S.B., Crespilho A.M., Dell’Aqua J.A., Jr., Miró J., Papa F.O., Alvarenga M.A.. Uterine clinical findings, fertility rate, leucocyte migration, and COX-2 protein levels in the endometrial tissue of susceptible mares treated with Platelets-rich plasma before and after AI. Theriogenology 2017;104:120–126.
    doi: 10.1016/j.theriogenology.2017.08.007pubmed: 28822903google scholar: lookup
  17. Pietramaggiori G., Kaipainen A., Ho D., Orser C., Pebley W., Rudolph A., Orgill D.P.. Trehalose lyophilized Platelets for wound healing. Wound Repair Regen. 2007;15:213–220.
    doi: 10.1111/j.1524-475X.2007.00207.xpubmed: 17352753google scholar: lookup
  18. Pietramaggiori G., Kaipainen A., Czeczuga J.M., Wagner C.T., Orgill D.P.. Freeze-dried Platelets-rich plasma shows beneficial healing properties in chronic wounds. Wound Repair Regen. 2006;14:573–580.
    doi: 10.1111/j.1743-6109.2006.00164.xpubmed: 17014669google scholar: lookup
  19. Sum R., Hager S., Pietramaggiori G., Orgill D.P., Dee J., Rudolph A., Orser C., Fitzpatrick G.M., Ho D.. Wound healing properties of trehalose-stabilized freeze-dried outdated Platelets. Transfusion 2007;47:672–679.
    doi: 10.1111/j.1537-2995.2007.01170.xpubmed: 17381626google scholar: lookup
  20. Arguelles D., Carmona J.U., Pastor J., Iborra A., Viñals L., Martínez P., Bach E., Prades M.. Evaluation of single and double centrifugation tube methods for concentrating equine Platelets. Res. Vet. Sci. 2006;81:237–245.
    doi: 10.1016/j.rvsc.2005.12.008pubmed: 16969921google scholar: lookup
  21. López C., Carmona J.U., Samudio I.. Use of autologous concentrate plasma for the treatment of a scapular fracture and brachial plexus injury caused by shot in a horse. Arch. Med. Vet. 2010;42:209–214.
  22. Risvanli A., Kaygusuzoglu E.. Immunological infertility in domestic animals. Turk. Vet. Med. 2004;3–4:52–55.
  23. Garverick H.A., Zollers W.G., Smith M.F.. Mechanisms associated with corpus luteum lifespan in animals having normal or subnormal luteal function. Anim. Reprod. Sci. 1992;28:111–124.
    doi: 10.1016/0378-4320(92)90098-Xgoogle scholar: lookup
  24. Copelin J.P., Smith M.F., Garverick H.A., Youngquist R.S.. Effect of the uterus on subnormal luteal function in anestrous beef cows. J. Anim. Sci. 1987;64:1506–1511.
    doi: 10.2527/jas1987.6451506xpubmed: 3583954google scholar: lookup
  25. Lishman A.W., Inskeep E.K.. Deficiencies in luteal function during reinitiation of cyclic breeding activity in beef cows and in ewes. J. Anim. Sci. 1991;21:59–75.
  26. Rantala M.H., Mutikainen M., Schuler G., Katila T., Taponen J.. Endometrial expression of progesterone, estrogen, and oxytocin receptors and of 20a-hydroxysteroid dehydrogenase and cyclooxygenase II 2 and 5 days after ovulation in induced short and normal estrous cycles in dairy cows. Theriogenology 2014;81:1181–1188.
    doi: 10.1016/j.theriogenology.2014.01.024pubmed: 24793300google scholar: lookup
  27. Pazoki H., Eimani H., Farokhi F., Shahverdi A.H., Salman Yazdi R., Tahaei L.S.. Comparing the growth and the development of mouse pre-antral follicle in medium with PL (Platelets Layset) and with FBS. Middle East. Fertil. Soc. J. 2015;20:231–236.
    doi: 10.1016/j.mefs.2015.01.006google scholar: lookup
  28. Cremonesi F., Bonfanti S., Idda A., Lange-Consiglio A.. Platelets Rich Plasma for Regenerative Medicine Treatment of Bovine Ovarian Hypofunction. Front. Vet. Sci. 2020;7:517.
    doi: 10.3389/fvets.2020.00517pmc: PMC7438405pubmed: 32903508google scholar: lookup
  29. Hosseini L., Shirazi A., Naderi M.M., Shams-Esfandabadi N., Boroujeni S.B., Ali Sarvari A., Sadeghnia S., Behzadi B., Akhondi M.M.. Platelets-rich plasma promotes the development of isolated human primordial and primary follicles to the preantral stage. Reprod. Med. Online. 2017;35:343–350.
    doi: 10.1016/j.rbmo.2017.04.007pubmed: 28756131google scholar: lookup
  30. Dehghani F., Aboutalebi H., Esmaeilpour T., Panjehshahin M.R., Bordbar H.. Effect of Platelets-rich plasma (PRP) on ovarian structures in cyclophosphamide-induced ovarian failure in female rats: A stereological study. Toxicol. Mech. Methods. 2018;28:653–659.
    doi: 10.1080/15376516.2018.1491662pubmed: 29968488google scholar: lookup
  31. Metcalf E.S.. The effect of Platelets-Rich Plasma (PRP) on intraluminal fluid and pregnancy rates in mares susceptible to Persistent Mating-Induced Endometritis (PMIE). J. Equine Vet. Sci. 2014;34:128.
    doi: 10.1016/j.jevs.2013.10.087google scholar: lookup
  32. Wang X., Liu L., Mou S., Zhao H., Fang J., Xiang Y., Zhao T., Sha T., Ding J., Hao C.. Investigation of Platelets-rich plasma in increasing proliferation and migration of endometrial mesenchymal stem cells and improving pregnancy outcome of patients with thin endometrium. J. Cell Biochem. 2019;120:7403–7411.
    doi: 10.1002/jcb.28014pubmed: 30506864google scholar: lookup
  33. Chang Y., Li J., Wei L.N., Pang J., Chen J., Liang X.. Autologous Platelets-rich plasma infusion improves clinical pregnancy rate in frozen embryo transfer cycles for women with thin endometrium. Medicine 2019;98:14062.
    doi: 10.1097/MD.0000000000014062pmc: PMC6370111pubmed: 30653117google scholar: lookup
  34. Coksuer H., Akdemir Y., Barut M.U.. Autologous Platelets-rich plasma treatment in infertility patients with RIF improve IVF success. Gynecol. Endocrinol. 2019;35:815–818.
    doi: 10.1080/09513590.2019.1597344pubmed: 30966843google scholar: lookup
  35. Zamaniyan M., Peyvandi S., Gorji H.H., Moradi S., Jamal J., Aghmashhadi F.Y.P., Mohammadi M.H.. Effect of Platelets-rich plasma on pregnancy outcomes in infertile women with recurrent implantation failure: A randomized controlled trial. Gynecol. Endocrinol. 2020;2:1–5.
    doi: 10.1080/09513590.2020.1756247pubmed: 32363968google scholar: lookup
  36. Metcalf E.S., Scoggin K., Troedsson M.H.T.. The effect of Platelets-rich plasma on endometrial pro-inflammatory cytokines in susceptible mares following semen deposition. J. Equine Vet. Sci. 2012;32:498.
    doi: 10.1016/j.jevs.2012.06.065google scholar: lookup
  37. Marini M.G., Perrini C., Esposti P., Brorradetti B., Bizzaro D., Riccaboni P., Fantinato E., Urbani G., Gelati G., Cremonesi F.. Effects of Platelets-rich plasma in a model of bovine endometrial inflammation in vitro. Reprod. Biol. Endocrinol. 2016;14:58.
    doi: 10.1186/s12958-016-0195-4pmc: PMC5020481pubmed: 27619959google scholar: lookup
  38. Messaoudi S., El Kasmi I., Bourdiec A., Crespo K., Bissonnette L., Le Saint C., Bissonnette F., Kadoch I.J.. 15 years of transcriptomic analysis on endometrial receptivity: What have we learnt?. Fertil. Res. Prac. 2019;5:9.
    doi: 10.1186/s40738-019-0059-7pmc: PMC6681490pubmed: 31396393google scholar: lookup
  39. Kim H., Shin J.E., Koo H.S., Kwon H., Choi D.H., Kim J.H.. Effect of autologous Platelets-rich plasma treatment on refractory thin endometrium during the frozen embryo transfer cycle: A pilot study. Front. Endocrinol. 2019;10:61.
    doi: 10.3389/fendo.2019.00061pmc: PMC6382681pubmed: 30837945google scholar: lookup

Citations

This article has been cited 5 times.
  1. Mohamed A, Fathi M, Shamaa AA, El Shahat KH. Impact of autologous platelet-rich plasma used for treatment of oligozoospermia in dogs on the quality of semen and testicular blood flow. Vet Res Commun 2025 May 1;49(3):182.
    doi: 10.1007/s11259-025-10734-8pubmed: 40310580google scholar: lookup
  2. Abdoon ASS, Al-Atrash AME, Soliman SS, El-Sanea AM, Gamal El Din AA, Fahmy HM. Lyophilized equine platelet-rich plasma (L-GF(equina)) antagonize the Reproductive toxicity and oxidative stress Induced by Cyclophosphamide in female rats. J Ovarian Res 2023 Apr 28;16(1):84.
    doi: 10.1186/s13048-023-01161-xpubmed: 37118826google scholar: lookup
  3. Borş SI, Dascălu DL, Borş A, Fahmy HM, Kandil OM, Abdoon ASS. Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF(equina)) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows. Animals (Basel) 2022 Sep 29;12(19).
    doi: 10.3390/ani12192618pubmed: 36230359google scholar: lookup
  4. Morrell JM, Rocha A. A Novel Approach to Minimising Acute Equine Endometritis That May Help to Prevent the Development of the Chronic State. Front Vet Sci 2021;8:799619.
    doi: 10.3389/fvets.2021.799619pubmed: 35071389google scholar: lookup
  5. Fantini P, Jiménez R, Vilés K, Iborra A, Palhares MS, Catalán J, Prades M, Miró J. Simple Tube Centrifugation Method for Platelet-Rich Plasma (PRP) Preparation in Catalonian Donkeys as a Treatment of Endometritis-Endometrosis. Animals (Basel) 2021 Oct 9;11(10).
    doi: 10.3390/ani11102918pubmed: 34679937google scholar: lookup
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