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Animals : an open access journal from MDPI2021; 11(4); doi: 10.3390/ani11041123

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares.

Abstract: This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GFequina) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phase; and the third group (L-GFequina Group), consisting of mares that were intrauterine infused with 20 mL of reconstituted lyophilized horse platelets growth factors (L-GFequina) on the second day after the estrus phase. The results showed no significant difference between control and treated groups in the diameter of the preovulatory follicles during the post treatment cycle. The endometrium thickness increased significantly in the L-GFequina and PRP groups ahead of the non-treated group. Intrauterine L-GFequina or PRP administration shortened the estrus cycle length. A higher pregnancy rate was recorded in the L-GFequina and PRP treated mares. In conclusion, the intrauterine infusion of L-GFequina or PRP increased the endometrial thickness and pregnancy rate and could be used to improve fertility in Arabian purebred mares who experienced from repeat breeding.
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Publication Date: 2021-04-14 PubMed ID: 33919901PubMed Central: PMC8070911DOI: 10.3390/ani11041123Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated if the infusion of substances like platelet-rich plasma (PRP) or lyophilized growth factor (L-GF) into the uterus of Arabian mares could enhance fertility. Following the infusion, beneficial effects were observed in the thickness of the uterine lining and pregnancy rates, suggesting these treatments could potentially improve fertility in repeat breeding mares.

Objective and Methodology

  • The researchers aimed to explore the impact of injecting PRP or L-GF into the uterus of Arabian mares on follicular growth, thickness of the uterine lining, length of estrus cycle, and pregnancy rates. This was intended to formulate potential solutions for infertility in mares that had tried breeding multiple times without success.
  • A total of 73 Arabian mares who had tried breeding for three cycles without success were split randomly into three groups. The control group remained untreated, one group was administered with PRP after the estrus phase, and the third group with L-GF following the estrus phase.

Findings and Conclusions

  • Observations post the treatment cycle did not show a significant difference in the diameter of preovulatory follicles between the control and treated groups.
  • A significant increase was noted in the thickness of the uterine lining in the L-GF and PRP groups as compared with the non-treated group. The thickness of the uterine lining is a critical factor in the successful implantation of the embryo, thus influencing fertility.
  • Both L-GF and PRP administration led to a shortening of the estrus cycle. This is a positive outcome as a shorter estrus cycle is typically associated with improved fertility in mares.
  • Increased pregnancy rates were observed in the mares treated with L-GF and PRP. The positive effect of these substances on pregnancy rates suggests improvement in fertility.
  • In conclusion, the study indicated that the intrauterine infusion of L-GF or PRP could increase the thickness of the uterine lining and pregnancy rates, thereby potentially offering an effective method of enhancing fertility in Arabian mares who experience repeat unsuccessful breeding cycles.

Cite This Article

APA
Dawod A, Miro J, Elbaz HT, Fahmy H, Abdoon AS. (2021). Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares. Animals (Basel), 11(4). https://doi.org/10.3390/ani11041123

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 4

Researcher Affiliations

Dawod, Ahmed
  • Husbandry and Animal Wealth Development Department, Faculty of Veterinary Medicine, University of Sadat City, Menofia 32897, Egypt.
Miro, Jordi
  • Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, E-08193 Barcelona, Spain.
Elbaz, Hamed T
  • Theriogenology Department, Faculty of Veterinary Medicine, University of Sadat City, Menofia 32897, Egypt.
Fahmy, Hossam
  • Faculty of Medicine, Ain Shams University, Cairo 11341, Egypt.
Abdoon, Ahmed S
  • Department of Animal Reproduction and Artificial Insemination, National Research Center, Giza 12622, Egypt.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 5 times.
  1. Mohamed A, Fathi M, Shamaa AA, El Shahat KH. Impact of autologous platelet-rich plasma used for treatment of oligozoospermia in dogs on the quality of semen and testicular blood flow. Vet Res Commun 2025 May 1;49(3):182.
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